Twist Bioscience’s Post

RT-qPCR is a powerful tool in molecular diagnostics, however, it is not without its challenges. Understanding and addressing these challenges is key to enhancing the efficiency and accuracy of RNA-based assays. . . . . . . #qpcr #assaydevelopment #moleculardiagnostics #pcr #pcrkits #assays #biology #science #research #trending #scienceandtechnology #rna #dna #pcrassays #dnasoftware https://lnkd.in/epqz62XH

Harnessing synthetic biology for advancing #RNA therapeutics and #vaccine design:- •Understanding RNA-protein interactions is crucial for deciphering the cellular functions and molecular mechanisms of regulatory RNAs. •Consequently, there is a constant need to develop innovative and cost-effective methods to uncover such interactions. •We developed a simple and cost-effective technique called Multiple Oligo assisted RNA Pulldown via Hybridization (MORPH) to identify proteins interacting with a specific RNA. •#MORPH employs a tiling array of antisense oligos (#ASOs) to efficiently capture the RNA of interest along with proteins associated with it. •Unlike existing techniques that rely on multiple individually biotinylated oligos spanning the entire RNA length, MORPH stands out by utilizing a single biotinylated oligo to capture all the ASOs. •To evaluate MORPH’s efficacy, we applied this technique combined with mass spectrometry to identify proteins interacting with #lncRNA NEAT1, which has previously been studied using various methods. •Our results demonstrate that despite being a simple and inexpensive procedure, #MORPH performs on par with existing methods. #rnatherapeutics #oligonucleotides #vaccinedevelopment #vaccineresearch

Today at 5 PM CET!

🧬 Exciting breakthrough in genome editing! Researchers have demonstrated a novel approach to achieve human cell-specific genome editing both ex vivo and in vivo using engineered extracellular vesicles (EDVs) to deliver molecular cargo. This advancement could revolutionize therapeutic interventions involving genome editing, providing a safe and effective delivery method to target cell nuclei. Learn more about this cutting-edge research here: https://lnkd.in/e_UhXgsZ #genomeediting #biotechnology #researchbreakthrough 🌟

Check out this new exciting review article from Amgen about RNATAC!

Comparative Analysis of Commercial Single-Cell RNA Sequencing Technologies This study evaluates ten commercially available single-cell RNA sequencing (scRNA-seq) approaches across four technology groups: Emulsion-based kits from 10x Genomics and Fluent Biosciences; Microwell-based kits from Becton Dickinson, Honeycomb Technologies and Singerlon Technologies; Combinatorial-indexing kits from Parse Biosciences and Scale Biosciences; and a Matrigel-based kit from Scipio Biosciences. Peripheral blood mononuclear cells (PBMCs) from a single donor were used to assess analytical performance. Key features such as sample compatibility, cost, and experimental duration were also compared. Notably, superior analytical performance was demonstrated by the Chromium Fixed RNA Profiling kit from 10x Genomics, which uniquely features probe hybridization for transcript detection. Additionally, the Rhapsody WTA kit from Becton Dickinson provided a cost-effective balance of performance and expense per cell. With a rich dataset of 218,154 cells, this work provides a basis for differentiating commercial scRNA-seq technologies, which is intended to facilitate the effective application and further methodological development of single cell transcriptomics.   Edmund Wong Thanks for the sharing ! #scRNA #scWTA #scMultiomics #SingleCell https://lnkd.in/gCGNJgrs

This is game changing. Alphafold 3 has the ability to include the interactions between the DNA, transcription factors, RNA, and other molecules. It will expedite drug discovery and development. https://lnkd.in/gMPXrU6M

Anyone who has done flow knows that BD has been a leader in the single cell protein analysis space for a long time. Really exciting to see the years of expertise brought to our Rhapsody single-cell system with our new IC CITE-seq assay--simultaneous RNA, surface protein and intracellular protein detection at the single-cell level with unbiased cell capture so you can get the most info from your precious cells (even the finicky, fragile ones!). What additional insights could this provide you with your experiments? Let's talk it through together and see. #singlecellanalysis #scRNAseq #CITEseq #nocellsleftbehind

Register for the upcoming webinars this week! https://lnkd.in/dhmwB2h Qlucore Omics Explorer: Single cell RNA-seq data analysis using the 10x Genomics import pipeline (Mar 14) The program speed and rich visualizations make single cell data analysis easier. In this webinar we will look at import and normalization of H5 files from 10X Genomics CellRanger, using the Qlucore inbuilt pipeline. We will also look at the visualization of the data in UMAP plots, and show how you can use annotation tools including shapes and colors to differentiate between groups. Qlucore Insights: Unbiased analysis of fusion genes: The importance for clinical decisions (Mar 15) Today, about 20 treatments approved by the FDA and EMA are directed toward specific cancer-related gene fusion, and the number of drugs grows annually. The importance of gene fusions in oncology grows from year to year which follows a demand for scalable solutions from diagnostic laboratories. RNA sequencing is the most informative approach to analyzing gene fusions. Moreover, while DNA is largely identical in all cells, RNA fluctuates considerably both in presence and relative concentrations. The variation reflects cellular characteristics or phenotype, and thus RNA analysis gives a more accurate view of the state cells are in compared to DNA analysis. #qlucore #webinar #qlucoreomicsexplorer #qlucoreinsights #bioinformatics #singlecell #rnaseq

🚀 Introducing Our Recombinant Murine RNase Inhibitor! 🧬 We are excited to unveil our latest innovation in RNA research—our Recombinant Murine RNase Inhibitor! Designed to support your most challenging experiments, this powerful inhibitor offers exceptional benefits: 🔬 Exceptional Endurance: Exhibits outstanding tolerance to pH and temperature shifts, ensuring consistent performance under various conditions. 💡 Superior Stability: Boasts unparalleled robustness, making it the reliable choice for RNA protection in dynamic environments. 🧪 Cutting-Edge Reliability: Engineered for maximum efficiency, our RNase inhibitor ensures the integrity of your RNA samples, leading to more accurate and reproducible results. 🌟 Versatile Application: Ideal for a wide range of applications, including RT-PCR, in vitro transcription, and more. Elevate your RNA research with a product that combines cutting-edge science with practical reliability. Discover the difference our Recombinant Murine RNase Inhibitor can make in your lab! #RNAResearch #BiotechInnovation #LaboratoryExcellence #ScientificBreakthrough #RNaseInhibitor #Lupexbiotechnologies #cDNAsynthesis #genetics #molecularbiology #qPCR #Biotechnology #GeneticResearch #ResearchAndDevelopment #enzymes #bestenzymes #genomicresearch