Vesiculab’s Post

View organization page for Vesiculab, graphic

8,310 followers

Bacteria-derived extracellular vesicles stimulate tumor growth in renal cell carcinoma: in this work, Kentaro Jingushi, Atsunari Kawashima and collaborators at Osaka University conducted 16S rRNA metagenomic analysis on bacterial DNA present in serum extracellular vesicles from healthy donors and renal cell carcinoma patients, identifying Cutibacterium acnes DNA as a distinctive bacterial component https://lnkd.in/eHMyUNve Their findings indicate that extracellular vesicles released by C. acnes present in renal cell carcinoma tissues exert tumor-promoting effects. An article also authored by Sayaka Tanikawa, Takuro Saito, Akinaru Yamamoto, Toshihiro Uemura, Nesrine Sassi, Yu Ishizuya, Yoshiyuki Yamamoto, Taigo Kato, Koji Hatano, Hiroaki Hase, Norio Nonomura and Kazutake Tsujikawa #extracellularvesicles #OMV #bacteria #carcinoma #Vesiculab

  • Top image: Cutibacterium acnes releases extracellular vesicles (EVs) with its genomic DNA. (A) A representative image of C. acnes culture. (B) EM image of C. acnes EVs. (C) NTA of C. acnes EVs. (D) PCR targeting 16S rRNA gene of C. acnes in C. acnes EVs
Bottom image: Cutibacterium acnes extracellular vesicles (EVs) promote renal cell carcinoma tumor growth in vivo. PKH26-labeled PBS (A), C. acnes EVs (B), or E. coli EVs (C) were intraperitoneally administered to mice with RenCa tumors and subjected to immunofluorescence analysis. (D) The effect of C. acnes EVs on RenCa cell tumor growth. (E) Immunohistochemistry (IHC) of RenCa cell tumor using anti-Ki67 and anti-CD31 antibodies. % of Ki67-positive (F) and CD31-positive (H) cells and % of Ki67 (G) and CD31 (I) highly positive cells. (J). (K) IHC of RenCa cell tumor. % of Ki67-positive (L) and CD31-positive (N) cells and % of Ki67 (M) and CD31 (O) highly positive cells.
Genevieve Bart

Senior Research Fellow, life science, Cell Biology

5mo

When we thought that taking the whole patient into account would help understand and cure cancer, it meant the whole ecosystem.  Probably some work is needed to go beyond diagnostic with this. 

To view or add a comment, sign in

Explore topics