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Enhancing bone-repair using LNP-mRNA: Chelsea Bahney, Nicole Ehrhart at Colorado State University and collaborators enhanced a delivery platform for a mRNA-based therapeutic encoding β-catenin by leveraging recent advances in liposomal technologies, using two FDA-approved ionizable phospholipids (MC3 and SM-102) to create unique ionizable lipid nanoparticle formulations. These LNPs were subsequently tested for transfection efficacy both in vitro and in a murine tibia fracture model https://lnkd.in/eYSMSvhY SM-102 LNPs demonstrated superior transfection efficacy in vitro and provided prolonged transfection in vivo, with minimal interference in fracture healing and no localized inflammatory response, outperforming MC3 LNPs. An article also authored by Anna Laura Nelson, Chiara Mancino, PhD, Xueqin Gao, Joshua C., Laura Chubb, Katherine Williams, Molly Czachor, Ralph Marcucio, Francesca Taraballi, John Cooke and Johnny Huard #LNP #mRNA #drugdelivery #targeteddelivery #genetherapy #Vesiculab

  • Top image: Characterization of MC3 and SM-102 ionizable LNPs. A). Schematic representation of LNPs, structure of ionizable lipids SM-102 and MC3, and characteristics, B). transfection efficacy of firefly luciferase loaded LNPs, and C). metabolic activity of LNPs in vitro. Schematic created with Biorender.com
Bottom image: Histomorphometry was performed to quantify bone and cartilage composition for all treatment groups 2 weeks after fracture. Representative images of each treatment group of A). the entire callus region (40X), B). an overview of the callus region (40X), C). newly formed trabecular bone (200X) and D). cartilage regions within the callus (200X). The regions were quantified using standard histomorphometric principles and the percent area of each region can be viewed in E). for bone and F). for cartilage. Scale bars at 40X magnification represent 500 μm and at 200X they represent 100 μm.

Thanks for the very interesting information or article.

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