Vishnu Modur, Ph.D.’s Post

Are you looking for a highly reproducible, reliable IN VITRO model of the small human intestine for your chemical, drugs, and metabolical studies? Do not miss the opportunity to attend MatTek Life Sciences workshop during 𝗧𝗵𝗲 𝟮𝟱𝘁𝗵 𝗜𝗻𝘁𝗲𝗿𝗻𝗮𝘁𝗶𝗼𝗻𝗮𝗹 𝗦𝘆𝗺𝗽𝗼𝘀𝗶𝘂𝗺 𝗼𝗻 𝗠𝗶𝗰𝗿𝗼𝘀𝗼𝗺𝗲𝘀 𝗮𝗻𝗱 𝗗𝗿𝘂𝗴 𝗢𝘅𝗶𝗱𝗮𝘁𝗶𝗼𝗻𝘀 𝗶𝗻 𝗣𝗿𝗮𝗴𝘂𝗲, 𝗳𝗿𝗼𝗺 𝗝𝘂𝗹𝘆 𝟳-𝟭𝟬, 𝟮𝟬𝟮𝟰. Dr. Jan Markus will present you the 𝗘𝗽𝗶𝗜𝗻𝘁𝗲𝘀𝘁𝗶𝗻𝗮𝗹, the first of its kind reconstructed human small intestine model, in the practical hands-on demonstration. https://lnkd.in/eDXDgHB3

They write: Recently, we discovered that WD repeat-containing protein 5 (WDR5) binds to MYC and is a critical cofactor required for the recruitment of MYC to its target genes and reported the first small molecule inhibitors of the WDR5–MYC interaction using structure-based design. These compounds display high binding affinity, but have poor physicochemical properties and are hence not suitable for in vivo studies. Herein, we conducted an NMR-based fragment screening to identify additional chemical matter and, using a structure-based approach, we merged a fragment hit with the previously reported sulfonamide series. Compounds in this series can disrupt the WDR5–MYC interaction in cells, and as a consequence, we observed a reduction of MYC localization to chromatin.

The importance of accurate glycan analysis for multispecifics, article here:https://lnkd.in/ewquQVeH “While using top down, sub-unit, or peptide mapping methods and state-of-the-art MS systems enables comprehensive glycan analysis of multispecifics since it provides the most information in a single step, including molecular weight, domain/site localization, species identity, and species quantity, most organizations employ multiple analytical approaches to support their characterization efforts and obtain secondary, confirmatory evidence,” says Orens. “The best way to ensure accurate and comprehensive characterization,” agrees Potter, “is to use more than one orthogonal method. Released glycan analysis, intact mass LC/MS, and LC/MS after protease digestion needn’t be mutually exclusive—they can be complementary,” he concludes. Thanks to Paula Orens Oscar Potter SCIEX Agilent Technologies and Cynthia Challener BioPharm International #antibody #mAbs #massspec

Modified and unmodified oligonucleotides are efficiently separated and purified through ion pair reversed phase (IP-RP) separations. Downstream purification of oligos presents challenges due to a variety of reasons, for example impurities can be very similar to the target molecules. In this application note, we highlight the use of a batch-tested preparative column specifically designed for oligos, combined with HFIP-free mobile phase. This strategy provides a new level of assurance for the development of high throughput, high recovery purifications of CRISPR sgRNA, siRNA and oligo-conjugates. Read the application note here: https://bit.ly/3ZBZfCL

💊 A pharmacophore is like a molecular map, highlighting the essential steric and electronic features of a ligand needed for optimal interactions with a specific biological target. 3D-pharmacophores can then be used to perform a screening of available databases. 🔍 The selected databases could undergo an initial screening for drug-like molecules, applying rational filters such as Lipinski’s Rule or drug-like ADME properties. This ensures we're navigating towards promising compounds with therapeutic potential. #Pharmacophores #DrugDiscovery #MolecularInteraction #InSilicoScience #3DPharmacophores #PharmaExploration #ResearchJourney #ScientificInnovation#ComputationalScience #MolDrugAI #HomologyModeling

Dive into the world of membrane protein targets. How to utilize Grating Coupled Interferometry GCI for complex membrane proteins

Just Published 🧬 Cyclic Ion Mobility to Characterize Modified Oligos ⁉Have you seen this? What happens when teams led by Professor David Clemmer of Indiana University Bloomington and Todd Maloney from Eli Lilly and Company collaborate? They apply ion mobility to tease apart new molecular details of pharmaceutically relevant samples, of course. 📑 "Diastereomer Characterization of PS-Modified Synthetic Oligonucleotides Using Cyclic IMS-MS" Check it out for yourself: https://lnkd.in/ggffFVvx Highlights: - Direct analysis of intact phosphorothioate (PS)-modified oligonucleotides - Resolution of diastereomer information from the combination of tandem MS and tandem IMS - New analytical options to better understand structure-function/pharmacological properties Authors: Edie Sharon, Sarah O’Keefe, Kathleen Grassmyer, Shannon Raab, Ph.D., Todd Maloney, and David Clemmer #Oligonucleotides #MassSpectrometry #IonMobility #AnalyticalChemistry #ASOs #siRNA

The NanoBRET™ Target Engagement (TE) method is a live-cell compound binding assay, developed in collaboration with chemists and biologists to address the limitations of traditional assay technologies in drug discovery and development. NanoBRET™ TE uses bioluminescence resonance energy transfer (BRET) to measure compound binding to target proteins in live cells, delivering high-quality data that reflects a range of physiological conditions. https://bit.ly/3wY7AF2

If you're looking for improved ways to characterize your lipid nanoparticles (LNPs), join me for a webinar on analytical ultracentrifugation (AUC) and how it can help analyze several of your CQAs in a single experiment. The webinar will also examine AUC's ability to characterize RNA. It can determine purity and oligomerization states and whether multiple folding states are present, which could affect its stability and function.