Waters | Wyatt Technology’s Post

Perfect application of AF4! How can you know that your molecule forms large but delicate aggregates if an SEC column shears them apart?

New synthetic cannabinoids, based upon chemical modifications of Delta 9-THC, are becoming more prevalent in the illicit drug supply. Some changes, such as modifications to the alkyl chain, have significant impact on receptor binding and extending psychoactive effects. As many of these compounds are isobaric and isomeric forms, resolution and identification of these compounds with traditional techniques is difficult if not, impossible. Si Huang et al have recently published their work as Open Access in Analytical Chemistry (https://lnkd.in/g4zvZezr) where they use ion mobility and a Waters Cyclic IMS to resolve and identify some of these compounds in cannabis extracts and edibles. #LCMS4Forensics

HOW TO GET AN R2 VALUE OF 0.996… This experiment shows how flowbot ONE prepares a dsDNA standard curve for quantitation with Thermo Fisher Scientific’s Quant-iT™ PicoGreen™ dsDNA Reagent. In the protocol, the flowbot ONE dispensed volumes of TE buffer and dsDNA into a 96-well plate before adding a working solution of PicoGreen. A Bioshake 3000-T was integrated into the protocol to ensure thorough mixing. Check out method and results: https://lnkd.in/dzhD76e5 #dna #quantitation #labautomation #biotech #pharma

Understanding how small molecules can corral multimeric complexes is a key part of making efficient protein degraders and induced-proximity drugs. There's been some great insight from the Schulman & Ciulli groups in this area recently - read my thoughts on what their studies tell us at my latest blog here: https://lnkd.in/e7uSbKt4

𝗦𝗮𝗳𝗲 𝗮𝗻𝗱 𝗲𝗮𝘀𝘆-𝘁𝗼-𝗵𝗮𝗻𝗱𝗹𝗲 𝘁𝗿𝗶𝗳𝗹𝘂𝗼𝗿𝗼𝗺𝗲𝘁𝗵𝗼𝘅𝘆𝗹𝗮𝘁𝗶𝗻𝗴 𝗮𝗴𝗲𝗻𝘁: 𝗧𝗙𝗡𝗳 Trifluoromethyl nonafluorobutanesulfonate (TFNf), is safe and an easy-to-handle trifluoromethoxylating reagent. TFNF enables trifluoromethoxylation of electrophilic substrates; alkyl halides and triflates, unsaturated and aromatic compounds. It is useful for synthesis of biologically active compounds and drug discovery studies.   To learn more: https://lnkd.in/gNxFZnKV #tcichemicals #reagents #trifluoromethoxylatingagent #chemicals

Optimizing #Bioprocesspurification: Effective #nucleicacid removal in high salt environments As cell and #genetherapies advance, ensuring the purity and safety of biologics is crucial. Regulatory standards emphasize the need for effective removal of host cell nucleic acids, especially chromatin- associated #DNA. However, a major challenge is disrupting the robust #chromatin structure to achieve high purity levels. This month, join QIAGEN’s Joanna Niska-Blakie (Global Product Manager) and Eleonora Turco (Director, R&D Enzymes) as they introduce novel salt-active endonucleases that are highly efficient at removing #nucleicacidimpurities, including chromatin, and can advance #bioprocesspurification workflows. Register below:

Optimizing #Bioprocesspurification: Effective #nucleicacid removal in high salt environments As cell and #genetherapies advance, ensuring the purity and safety of biologics is crucial. Regulatory standards emphasize the need for effective removal of host cell nucleic acids, especially chromatin- associated #DNA. However, a major challenge is disrupting the robust #chromatin structure to achieve high purity levels. Next week, join QIAGEN’s Joanna Niska-Blakie (Global Product Manager) and Eleonora Turco (Director, R&D Enzymes) as they introduce novel salt-active endonucleases that are highly efficient at removing #nucleicacidimpurities, including chromatin, and can advance #bioprocesspurification workflows. Register below:

Oligos, Oligos, Oligos - Everywhere at HPLC 2024 This morning HPLC 2024 kicked off! Right away you will see #oligos and #nucleicacids everywhere. One of today's short course tracks is all about them. Claus Rentel from Ionis Pharmaceuticals, Inc. then presents a plenary where the audience will get the chance to soak in thought provoking insights. Later, on Mon and Wed, Kelly Zhang and Alexandre Goyon from Genentech give two keynotes on the subject. And that's just the tip of the iceberg on all the oligo science show and tell! Super excited to hear about the new ways #HPLC can be applied to assess physicochemical properties, assay purity, confirm identity, guide formulation, check sequences and modifications, and tease out important new structure-function relationships. 📘Final Program: https://lnkd.in/eE5S9vHw Like is listed in Dr. Rentel's plenary abstract: #antisense, #siRNA, #aptamer, #CRISPR, and #mRNA

🌍 💊 Last Tuesday, I had the privilege of attending the 3rd ICCPC International Conference on Contemporary Pharmacy Challenges, where I gained valuable insights into the latest trends in the pharmaceutical field. I also had the opportunity to present my oral presentation titled "SerotoninAI software as Disruptive Technology in Antidepressant Drug Discovery." During my talk, I introduced a new module of my SerotoninAI software, highlighting its focus on serotonin and norepinephrine transporters and its ability to predict pKi and pIC50 values for various molecules. I’m excited about the potential of SerotoninAI (https://lnkd.in/dkqTQqB6) to contribute to breakthroughs in antidepressant drug discovery. #drugdiscovery #QSAR #SERT #NET #antidepressant #SSRI #SNRI

This novel patent unveils conjugate molecules with a unique formula (I-a). In this formula, an active agent, specifically a COX-2 inhibitor moiety, is intricately linked to a cannabinoid moiety through a specialized linker. This linker is not merely a structural component but a pivotal feature that enables the conjugate molecule's targeted delivery and enhanced efficacy. https://lnkd.in/d6krrrKb