Yenifer Olivo Martínez’s Post

In today's #WomensHealthWednesday we take a look at a study from North Carolina State University that sheds light on the complexity of Gardnerella bacteria in the vaginal microbiome. Multiple species of Gardnerella, associated with conditions like bacterial vaginosis and pre-term birth, can coexist, revealing new avenues for understanding women's health. #WomensHealth #Microbiome #Microbiology

📃Scientific paper: Localization of EccA_3 at the growing pole in Mycobacterium smegmatis Abstract: Background Bacteria require specialized secretion systems for the export of molecules into the extracellular space to modify their environment and scavenge for nutrients. The ESX-3 secretion system is required by mycobacteria for iron homeostasis. The ESX-3 operon encodes for one cytoplasmic component (EccA_3) and five membrane components (EccB3 – EccE3 and MycP_3). In this study we sought to identify the sub-cellular location of EccA_3 of the ESX-3 secretion system in mycobacteria. Results Fluorescently tagged EccA_3 localized to a single pole in the majority of Mycobacterium smegmatis cells and time-lapse fluorescent microscopy identified this pole as the growing pole. Deletion of ESX-3 did not prevent polar localization of fluorescently tagged EccA_3, suggesting that EccA_3 unipolar localization is independent of other ESX-3 components. Affinity purification - mass spectrometry was used to identify EccA_3 associated proteins which may contribute to the localization of EccA_3 at the growing pole. EccA_3 co-purified with fatty acid metabolism proteins (FAS, FadA3, KasA and KasB), mycolic acid synthesis proteins (UmaA, CmaA1), cell division proteins (FtsE and FtsZ), and cell shape and cell cycle proteins (MurS, CwsA and Wag31). Secretion system related proteins Ffh, SecA1, EccA1, and EspI were also identified. Conclusions Time-lapse microscopy demonstrated that EccA3 is located at the growing pole in M. smegmatis . The co-purification of EccA_3 with proteins known... Continued on ES/IODE ➡️ https://etcse.fr/Lvx ------- If you find this interesting, feel free to follow, comment and share. We need your help to enhance our visibility, so that our platform continues to serve you.

Is there no end to the things the gut has an impact on? 😱 Take a look at the latest article on The Microbiologist, looking into some research on the relationship between the gut microbiome and aortic aneurism. Can't view the article? You might have reached your limit for the month for free articles! Create an account to get more, or sign up to be a member from just £6 to get free, unlimited access to all articles across our digital magazine. Membership options here: https://ow.ly/2rg050Rhkao Article: https://ow.ly/lgZy50Rhkan #GutHealth #Microbiome #HealthResearch #ScienceNews #Microbiology #Research

☑️ *READ ABSTRACT BELOW:* Keywords: Antioxidants; Dairy cows; Endocannabinoid system; Inflammation; Omega-3 fatty acids; Placenta. Part 1: Background: The placenta plays a crucial role in supporting and influencing fetal development. We compared the effects of prepartum supplementation with omega-3 (n-3) fatty acid (FA) sources, flaxseed oil (FLX) and fish oil (FO), on the expression of genes and proteins related to lipid metabolism, inflammation, oxidative stress, and the endocannabinoid system (ECS) in the expelled placenta, as well as on FA profile and inflammatory response of neonates. Late-pregnant Holstein dairy cows were supplemented with saturated fat (CTL), FLX, or FO. Placental cotyledons (n = 5) were collected immediately after expulsion, and extracted RNA and proteins were analyzed by RT-PCR and proteomic analysis. Neonatal blood was assessed for FA composition and concentrations of inflammatory markers. Results: FO increased the gene expression of fatty acid binding protein 4 (FABP4), interleukin 10 (IL-10), catalase (CAT), cannabinoid receptor 1 (CNR1), and cannabinoid receptor 2 (CNR2) compared with CTL placenta. Gene expression of ECS-enzyme FA-amide hydrolase (FAAH) was lower in FLX and FO than in CTL. Proteomic analysis identified 3,974 proteins; of these, 51-59 were differentially abundant between treatments (P ≤ 0.05, |fold change| ≥ 1.5). Top canonical pathways enriched in FLX vs. CTL and in FO vs. CTL were triglyceride metabolism and inflammatory processes Dos Santos Silva P, J Anim Sci Biotechnol. 2024 May 21;15(1):74. doi: 10.1186/s40104-024-01033-4. PMID: 38769527 #Gesundheit #Bildung #Fuehrung #Coaching #Mindset #Motivation #Gehirn #Neuroscience #Psychologie #Persoenlichkeitsentwicklung #Kindheit #KeyNoteSpeaker #Humangenetik #Biochemie #Neuroleadership #Ernaehrung #Transformation #Stress #Demografie #Gender #Age #interkulturelleKompetenz #Epigenetik #Veraenderung #EmotionaleIntelligenz #Change #Gesellschaft #Organisationsentwicklung #Philosophie #Beratung # Quantum

🔬 New Review on Fatty Acid Homeostasis! 🔬 I'm proud to share our newly published paper, "Disorders of Fatty Acid Homeostasis," in the Journal of Inherited Metabolic Disease. The paper provides a comprehensive review of fatty acid homeostasis, detailing the pathways and genetic disorders associated with fatty acid modifications and breakdown in human metabolism. A big thank you to the contributing authors: Sacha Ferdinandusse, Gajja Salomons and Ronald Wanders 📖 Read the full article here: https://lnkd.in/gRRE567U 👥 Feel free to share this research within your networks and discuss further! #MetabolicDiseases #GeneticDisorders #FattyAcidMetabolism #ScientificResearch #MedicalScience

Janthinobacterium lividum is an aerobic, Gram-negative, soil-dwelling bacterium that has a distinctive dark-violet (almost black) color, due to a compound called violacein, which is produced when glycerol is metabolized as a carbon source. Violacein has antibacterial, antiviral, and antifungal properties. Its antifungal properties are of particular interest, since J. lividum is found on the skin of certain amphibians, including the red-backed salamander (Plethodon cinereus), where it prevents infection by the devastating chytrid fungus (Batrachochytrium dendrobatidis). #microbiology #bacteriology #Violacein #violet #bacteria

I am very excited to share part of my thesis work that was published today in Proceedings of the National Academy of Sciences PNAS!! One of the hallmarks of aging is loss of proteostasis and chaperone-mediated autophagy (CMA), a selective form of autophagy plays an important role in maintaining the proteostasis and proteome remodeling, however, CMA activity declines with age. Studies have shown the beneficial effects of calorie restriction (CR) as an intervention to slow down aging and expand healthy lifespan. In this work we demonstrate that: 1. CMA is constitutively activated with calorie restricted (CR), an intervention that has been shown to increase healthspan, in the liver of old rodents and in in-vitro models of CR with cultured fibroblasts. 2. This upregulation of CMA by CR is due to improved stability of LAMP2A, the receptor of CMA, at the surface of the lysosome membrane. 3. Acute treatment of old mice with CR mimetics (CRMs), pharmacologically active substances that reproduce the biochemical and functional effect of CR, activated CMA in several tissues. 4. CMA is necessary to achieve the protective effect of CRMs against nutritional stress induced by high carbohydrate diet (HCD). 5. Activation of CMA contributes to part of the beneficial effects mediated by CR and CRMs, which emphasizes the gerotherapeutic potential of this type of autophagy. https://lnkd.in/eYi89VpV

Types of #Bacterial #motility #Microbiology 🦠🦠🦠🦠 ·        Stately motility – Bacillus spp. ·        Darting motility – Vibrio cholera, Campylobacter jejuni ·        Active motility – Pseudomonas spp. ·        Tumbling motility – Listeria monocytogens ·        Corkscrew, extension-flexion motility – Spirochetes ·        Swarming motility – Proteus spp., Clostridium tetani ·  Gliding motility – Mycoplasma ·        Examples of non motile bacteria- Most cocci, Shigella and Klebsiella

Successfully isolated bacteria from an unidentified sample in our experiment. *️⃣Morphology study on Nutrients agar plates. 1. size : Pinpoint and large 2. colour of the colony: ✓circular = Unbroken , peripheral edge ✓irregular= intended , peripheral edge ✓rhizoid = root like , spreading growth 3. Margin : appearance of the outer edge of the colony. ✓ Lobate = marked indentation ✓ Undulate = wavy indentation ✓ Filamentous = thread like, spreading edge. 4. Elevation : colony growth is raised on the surface is described. ✓ Raised = slightly elevated ✓ convex = doma shaped elevation #bacteria #growth #microorganisms #identity #elevation #nutrientsagar #morphology #microbiology #bacteriology #biotechnology #disease #healthcare #clinicalresearch #clinical #pathologylab

Part 2: Quantitative reverse transcription PCR was employed to detect the Kelch-like-ECH-associated protein 1-Nuclear factor erythroid 2-related factor 2 (Keap1-Nrf2), B cell and T cell targeting markers, and anti-inflammatory/inflammatory cytokines from the spleen tissues of geese. The SCFAs were determined from the caecal chyme of geese by using gas chromatography. In this study, ryegrass-enriched gut microbiota such as Eggerthellaceae, Oscillospiraceae, Rikenellaceae, and Lachnospiraceae attenuated commercial diet-induced gut microbial alterations and spleen dysfunctions in geese. Ryegrass significantly improved the SCFAs (acetic, butyric, propionic, isovaleric, and valeric acids), AMPK pathway-activated Nrf2 redox signaling cascades, B cells (B220, CD19, and IgD), and T cells (CD3, CD4, CD8, and IL-2, with an exception of IL-17 and TGF-β) to activate anti-inflammatory cytokines (IL-4 and IL-10) and immunoglobulins (IgA, IgG, and IgM) in geese. In conclusion, ryegrass-improved reprogramming of the gut microbiota restored the spleen functions by attenuating LPS-induced oxidative stress and systemic inflammation through the gut-microbiota-spleen axis in geese. Ali Q, Ma S, Liu B, Niu J, Liu M, Mustafa A, Li D, Wang Z, Sun H, Cui Y, Shi Y. Supplementing Ryegrass Ameliorates Commercial Diet-Induced Gut Microbial Dysbiosis-Associated Spleen Dysfunctions by Gut-Microbiota-Spleen Axis. Nutrients. 2024 Mar 5;16(5):747. doi: 10.3390/nu16050747. PMID: 38474875; PMCID: PMC10935305. #Gesundheit #Bildung #Fuehrung #Coaching #Mindset #Motivation #Gehirn #Neuroscience #Psychologie #Persoenlichkeitsentwicklung #Kindheit #KeyNoteSpeaker #Humangenetik #Biochemie #Neuroleadership #Ernaehrung #Transformation #Stress #Demografie #Gender #Age #interkulturelleKompetenz #Epigenetik #Veraenderung #EmotionaleIntelligenz #Change #Gesellschaft #Organisationsentwicklung #Philosophie #Beratung # Quantum