Cell Probe 360: Optimizing Cell Therapy Solutions

The field of regenerative medicine has come into its own space over the last decade. While there are still naysayers, and flawed practices and products, the general consensus is headed in a positive direction for both efficacy, patient outcomes and general satisfaction by both the provider and the patient. Bone marrow aspirate is a powerful regenerative tool and serves as the premier option for autologous cell therapy (Imam, 2017). However, traditional bone marrow aspiration techniques are not only time consuming, but do not yield the highest amount of viable mesenchymal stem cells possible. Celling Biosciences (Austin, TX) is seeking to overcome these issues with our patented Cell Probe 360: a new take on an aspiration needle for enhancing the method of bone marrow collection. Derived in part to seamlessly replicate Dr. Philippe Hernigou's bone marrow aspiration technique, the Cell Probe 360 optimizes the quality of the bone marrow draw.

The Hernigou aspiration method obtains 300% more cells than traditional aspiration techniques, making it the gold standard of bone marrow aspiration (Hernigou, 2013). In this method, bone marrow is aspirated in quick, short pulls. A small syringe (ideally 10 mL) is attached to a traditional jamshidi needle and pulled back quickly until bone marrow fills the syringe to the 4-5 mL mark. Then, the small syringe is removed and replaced with a new one, which is again used to collect 4-5 mLs of bone marrow. After the second syringe is removed, the jamshidi is rotated 90 degrees. After 360 degrees of rotation, the needle is pulled back 2 cm above the original site to access additional cells. This process is repeated until 60 mL of bone marrow is collected.

The Cell Probe 360 simplifies the Hernigou aspiration method, decreasing the time of the procedure as well as limiting sterile breaks. Featuring a proprietary valve system built into the needle itself, only two syringes are needed throughout the entirety of the procedure: one to aspirate and the other to act as a reservoir. The smaller syringe aspirates the bone marrow during the procedure, and the integrated valve system allows the bone marrow to flow into the larger “reservoir” syringe as the fluid is dispensed. As a result, the smaller syringe does not need to be replaced and can continue to aspirate small quantities, preventing sterile breaks during the procedure. The Cell Probe 360 is also capable of rotation during use, accounting for the rotation used in the Hernigou method.

The Cell Probe 360 is a new way to optimize cell therapy solutions by maximizing stem cell recovery. It is a minimally invasive, easy to use method of obtaining autologous bone marrow. Designing products with the cells in mind has been the center of our research and development for decades. For more information about the Cell Probe 360 please email ppruitt@celling.com, pending release in 2024.

Imam et al. A systematic review of the concept and clinical applications of Bone Marrow Aspirate Concentrate in Orthopaedics. SICOT J. 2017;3:17.

 Hernigou et al. Benefits of small volume and small syringe for bone marrow aspirations of mesenchymal stem cells. Int Orthop. 2013 Nov; 37(11):2279-87.