What's new with CellEDIT

💡Did you miss our webinar?

Don't worry, you can watch the replay now! In this webinar, Dr. Tobias Beyer provided a comprehensive overview of the CellEDIT service and its unique in-house developed genome engineering workflow.

>> Watch The Replay Now!

Highlights

• The Unique CellEDIT Approach: Learn how we leverage intranuclear injection of CRISPR RNPs into single cells, enhancing control over the editing process while minimizing off-target effects.
• Efficient Editing of Hard-to-Transfect Cells: Discover how our specialized workflow efficiently onboards and edits challenging cell types, opening new avenues for disease-specific cell lines in drug discovery research.
• Practical Applications: Witness the power of the CellEDIT workflow as we demonstrate its effectiveness in generating HPRT1 knockouts in both immortalized C2C12 cells and hard-to-transfect SK-MES1 cells, all starting from a mere handful of cells. 

🔍 Overcoming the Hard-to-transfect Cell Line Hurdle - The Application Note

Key Findings

• The CellEDIT workflow was used to successfully generate 3 monoclonal HPRT1 knockouts in SK-MES-1 cell, a notoriously hard-to-transfect cell line.
• Genotype analysis of 22 clones revealed that 55% (12) of these were edited at the target site and 23% (5) of all clones displaying editing on all four alleles.
• Gentle delivery of CRISPR-RNPs through intranuclear injection ensured a survival rate of 65% in SK-MES-1 cells, thereby showcasing the highly controllable and gentle nature of the CellEDIT workflow and its potential for editing notoriously hard-to-transfect cells.

>> Download the application note

📆 Upcoming Conferences

🚩 AACR Annual Meeting - Apr. 5 - 10 | Booth #119 - San Diego, California, USA

>> More information available

🚩E-ACR Annual Meeting - Jun. 10 - 13 | Booth #69 - Rotterdam, The Netherlands

>> More information available

Do you have any questions about the CellEDIT service or your project?

Contact us directly at info@cytosurge.com!

Thank you for being a part of our growing community. Stay tuned for more updates!

Leslie @ Cytosurge