Nanion Technologies

𝗥𝗲𝗮𝗱𝘆 𝘁𝗼 𝗲𝗹𝗲𝘃𝗮𝘁𝗲 𝘆𝗼𝘂𝗿 𝘁𝗿𝗮𝗻𝘀𝗽𝗼𝗿𝘁𝗲𝗿 𝗿𝗲𝘀𝗲𝗮𝗿𝗰𝗵? 🚀 Imagine having access to a cutting-edge instrument that delivers unparalleled sensitivity, speed, and ease of use, transforming the way you approach electrogenic transporter and membrane pumps research. Our SURFE2R N1 does exactly that! This year, the SURFE2R N1 instrument grant is back: We are offering you the chance to use the 𝗦𝗨𝗥𝗙𝗘𝟮𝗥 𝗡𝟭 𝗶𝗻 𝘆𝗼𝘂𝗿 𝗹𝗮𝗯 𝗳𝗼𝗿 𝘀𝗶𝘅 𝗺𝗼𝗻𝘁𝗵𝘀, 𝗰𝗼𝗺𝗽𝗹𝗲𝘁𝗲𝗹𝘆 𝗳𝗿𝗲𝗲 𝗼𝗳 𝗰𝗵𝗮𝗿𝗴𝗲, including consumables and full assay development support. No more dealing with harsh radiolabeled compounds or time-consuming protocols. This is your opportunity to advance your membrane transporter research. Apply now and be at the cutting edge of scientific discovery! 📅 Application deadline: September 30th, 2024 🔗 Learn more and apply: https://ow.ly/64oA50SCfkm #ResearchGrant #SURFE2RN1 #TransporterResearch #Electrophysiology #SSME #MembraneBiophysics

Do astrocytes influence the maturation of hiPSC-derived neurons? Can these supportive cells truly accelerate neuronal development, and what does that mean for advancing neuroscience research? These questions were explored in our latest study in collaboration with the Fraunhofer Institute for Biomedical Engineering (IBMT). Using the SyncroPatch 384, we investigated the impact of astrocytes on the maturation of hiPSC-derived neurons. Here’s what we discovered: ➡️ Co-culturing hiPSC-derived neurons with astrocytes led to a significant increase in cell capacitance and the amplitude of NaV and KV currents, indicating a more mature neuronal state. ➡️ Neurons grown with astrocytes exhibited NaV and KV currents in 100% of cells, compared to just 60% in neuron-only cultures. ➡️ The activation and inactivation properties of NaV channels remained consistent, suggesting that maturation occurred without changes to the channel subtypes. ➡️ We observed both GABA and glycine responses in both conditions (along with small responses to acetylcholine, Bz-ATP, and glutamate in a subset of neurons cultured with and without astrocytes). These results indicate that co-culturing with astrocytes significantly enhances the electrophysiological properties of hiPSC-derived neurons, making them a more robust model for research. 👉 Check out the full details in our application note: https://lnkd.in/g4Tbd2nh #Neuroscience #DrugDiscovery #hiPSC #IonChannels #iPSC #neurons #astrocytes #PatchClamp

The voltage-gated sodium channel Nav1.7 plays a critical role in transmitting pain signals in nociceptive neurons. Genetic mutations in Nav1.7 can lead to various pain disorders, including extreme pain conditions and congenital insensitivity to pain. Despite its importance, targeting Nav1.7 for pain treatment has proven difficult due to its structural similarity with other sodium channel subtypes, which limits the effectiveness of small molecules and monoclonal antibodies. In a recent study, researchers developed a novel antigen design strategy to generate single-domain antibodies (VHHs) that specifically target the Nav1.7 channel. They selected a unique extracellular loop from Nav1.7 as the target antigen (a region of 70 amino-acid residues from the domain DI extracellular loop 3) and grafted it into the complementarity-determining region 3 (CDR3) loop of an inert llama VHH to stabilize the native 3D conformation of this DIE3IR loop in the absence of the rest of the hNav1.7 channel. The resulting recombinant protein was then used to isolate VHHs specific to the DIE3 loop. This approach enabled the production of VHHs capable of binding to Nav1.7 with high specificity and functional efficacy. The team employed several techniques, including molecular modeling, phage display, and electrophysiological assays using the high-throughput automated patch-clamp system SyncroPatch 384, to isolate and characterize VHHs with the desired properties. Among the identified candidates, VHH DI-D was shown to slow the deactivation kinetics of Nav1.7, reduce the firing of action potentials in pain-sensing neurons, and reverse hyperalgesia in rat and mouse models of pain. These findings highlight the potential of this new antigen design strategy to develop highly specific biologics for targeting ion channels, transporters, and GPCRs. -- Find the full article here: https://lnkd.in/dpF9Kg9e Learn more about the SyncroPatch 384, our newest generation automated patch clamp system: https://lnkd.in/eNUKXRuf   #Nav17 #electrophysiology #ionchannels #pain

Exciting moments at the #SPS meeting in San Diego! It was an honor to stand alongside Yasu Kanda, a leading expert in the field, in front of a joint Nanion/Stanford poster on identifying iPSC-CM phenotypes via impedance cell monitoring. This research holds great potential for advancing the parametrization of patient-derived iPSC-CMs, and I was thrilled to have an impactful conversation with Kanda-san. Collaborating and learning from such brilliant minds continues to inspire me as we push the boundaries of innovation in cardiac safety. #cardiacsafety #Innovation #Collaboration #Science #Progress #Networking #stemcells #impedance #AtlaZ #cellbasedassays

How much do we really know about red blood cells (RBCs) and their ion channels? What ion channels are expressed in RBCs? What are their physiological functions? And what are the challenges in studying them? Our latest webinar revealed surprising insights that could change how you see these tiny but powerful cells. Here are a few takeaways from this webinar: 𝗥𝗲𝗱 𝗕𝗹𝗼𝗼𝗱 𝗖𝗲𝗹𝗹𝘀: Though often called "membranes without much of a cell," RBCs make up about 70% of all cells in the human body, and they are more than just simple carriers. 𝗡𝗼𝗯𝗲𝗹-𝗪𝗶𝗻𝗻𝗶𝗻𝗴 𝗗𝗶𝘀𝗰𝗼𝘃𝗲𝗿𝗶𝗲𝘀: Did you know that the groundbreaking discovery of the 𝗮𝗾𝘂𝗮𝗽𝗼𝗿𝗶𝗻 channel happened in RBCs, earning Peter Agre a Nobel Prize? 𝗟𝗼𝘄 𝗖𝗵𝗮𝗻𝗻𝗲𝗹 𝗖𝗼𝘂𝗻𝘁𝘀: With only 2-3 functional Gardos channels per cell, studying these channels has always been a challenge. 𝗥𝗕𝗖𝘀 𝗮𝗻𝗱 𝗢𝘄𝗲𝗻 𝗛𝗮𝗺𝗶𝗹𝗹: RBCs were one of the first cell types tested with patch clamp. Owen Hamill recorded the first RBCs in 1981. 𝗣𝗮𝘁𝗰𝗵 𝗖𝗹𝗮𝗺𝗽𝗶𝗻𝗴 𝗖𝗵𝗮𝗹𝗹𝗲𝗻𝗴𝗲𝘀: Small, fragile, and with low channel counts, RBCs are notoriously tough to study manually. But automated patch clamp is making it easier! 𝗣𝘀𝗲𝘂𝗱𝗼 𝗔𝗰𝘁𝗶𝗼𝗻 𝗣𝗼𝘁𝗲𝗻𝘁𝗶𝗮𝗹𝘀: Lars Kaestner introduced the concept of "pseudo action potentials" in RBCs, suggesting that even non-excitable cells like RBCs can exhibit membrane potential fluctuations similar to action potentials. 𝗣𝗶𝗲𝘇𝗼𝟭: The Piezo1 channel plays a significant role in maintaining RBC volume, shape, and overall functionality. Mutations in Piezo1 have been linked to hereditary xerocytosis, a genetic condition that leads to dehydration of RBCs and results in hemolytic anemia. 𝗧𝗥𝗣𝗩𝟮 & 𝗦𝗶𝗰𝗸𝗹𝗲 𝗖𝗲𝗹𝗹: Sickle cell patients show increased TRPV2 channel activity in RBCs, leading to more calcium influx and triggering the Gardos effect – key to understanding cell dehydration. If you’re curious about all these insights, don’t miss the full webinar recording! 👉 𝗪𝗮𝘁𝗰𝗵 𝘁𝗵𝗲 𝗿𝗲𝗰𝗼𝗿𝗱𝗶𝗻𝗴 𝗻𝗼𝘄: https://lnkd.in/ez9fKDDH #RBCs #ionchannels #APC #electrophysiology #patchclamp Nicoletta Murciano Yeimar Portillo Artem Kondratskyi

𝗨𝗻𝗹𝗼𝗰𝗸𝗶𝗻𝗴 𝘁𝗵𝗲 𝗽𝗼𝘄𝗲𝗿 𝗼𝗳 𝗶𝗼𝗦𝗲𝗻𝘀𝗼𝗿𝘆 𝗻𝗲𝘂𝗿𝗼𝗻𝘀 𝘄𝗶𝘁𝗵 𝘁𝗵𝗲 𝗣𝗮𝘁𝗰𝗵𝗹𝗶𝗻𝗲𝗿 Human induced pluripotent stem cells (hiPSCs) are transforming biomedical research, providing a human-origin alternative for disease modeling and drug discovery. However, culturing hiPSC-derived neurons to maturity (4 weeks or more) can lower cell capture rates and overall success rates in automated patch clamp (APC) experiments, as these techniques require cells in suspension. In collaboration with bit.bio we’ve addressed this challenge. Using their cutting-edge opti-ox (optimised inducible overexpression) technology for cell programming, ioSensory Neurons achieve over 99% purity as sensory neurons by day 7 post-revival. When combined with our Patchliner system, these neurons demonstrate high capture and sealing rates, unlocking new possibilities in ion channel research. 𝗞𝗲𝘆 𝗳𝗶𝗻𝗱𝗶𝗻𝗴𝘀: ✔️ 100% capture rate of ioSensory Neurons in all wells. ✔️ Over 80% of cells achieved an RSeal of >500 MΩ, allowing for reliable voltage and current clamp measurements. ✔️ Robust recording of TTX-sensitive (TTX-s) and TTX-resistant (TTX-r) NaV currents. ✔️ Detection of multiple ligand-gated ion channels, enhancing the scope of electrophysiological studies. With these results, researchers can achieve high-quality, consistent data in electrophysiology, reducing the complexities of working with mature neurons. Want to learn more about studying ioSensory Neurons on the Patchliner? Read the full application note here: https://lnkd.in/dahhBSD4 #StemCell #hiPSC #Electrophysiology #PatchClamp #DrugDiscovery #Innovation #Patchliner #APC #Neurons

𝗨𝗻𝗱𝗲𝗿𝘀𝘁𝗮𝗻𝗱𝗶𝗻𝗴 𝗻𝗲𝘂𝗿𝗼𝗻𝗮𝗹 𝗮𝗰𝘁𝗶𝘃𝗶𝘁𝘆 𝘄𝗶𝘁𝗵 𝗲𝗹𝗲𝗰𝘁𝗿𝗼𝗽𝗵𝘆𝘀𝗶𝗼𝗹𝗼𝗴𝘆 🧠 Neuronal excitability is crucial for brain function, and understanding ion channels and transporters is key to developing therapies for neurological disorders. Models like primary neurons and those derived from induced pluripotent stem cells (iPSCs) have revolutionized how we study neuronal behavior in both health and disease. iPSC-derived neurons, in particular, enable personalized medicine by allowing researchers to study cells from different genetic backgrounds, model diseases like neurodegeneration, and screen potential therapies tailored to individual patients. A major area of focus is neurotransmitter transporters, which regulate synaptic transmission by clearing neurotransmitters from the synaptic cleft. Dysregulation of these transporters is linked to conditions like depression, anxiety, addiction, and neurodegenerative diseases. Advanced techniques, such as automated patch clamp and solid-supported membrane (SSM) electrophysiology, have revolutionized our understanding of ion channel and transporter function, providing detailed insights and enabling the development of targeted therapies. These techniques have significantly expanded our ability to study ion channels and transporters implicated in neurophysiology and various neurological disorders. Want to learn more about ion channels, and transporters in neurophysiology? Visit https://ow.ly/Ts4I50TFmfm #Neuroscience #Electrophysiology #iPSC #NeuroResearch #SynapticTransmission #Neurodegeneration #IonChannels

𝗡𝗲𝘅𝘁 𝗰𝗼𝗻𝗳𝗲𝗿𝗲𝗻𝗰𝗲 𝘀𝘁𝗼𝗽: 𝗙𝗲𝘀𝘁𝗶𝘃𝗮𝗹 𝗼𝗳 𝗕𝗶𝗼𝗹𝗼𝗴𝗶𝗰𝘀! We’re excited to be heading to Basel for the Festival of Biologics, an event that brings together leading experts across the biologics value chain to drive innovation and improve patient health. The conference covers every stage of biologics, from discovery and development to manufacturing and market access. Be sure to visit us at booth #239, where Giovanna De Filippi and Elena Dragicevic will showcase the capabilities of our AtlaZ platform. Whether your research focuses on immunotherapies, GPCR signaling, cell adhesion and proliferation, cytotoxicity, morphology, barrier function, or other real-time applications, we’d love to explore how AtlaZ can enhance your work. Don’t miss our poster session either! We’ll present “AtlaZ as a High-Throughput System for Advanced Functional Analysis in Immunotherapy Development,” and we’re eager to discuss its potential with you. Curious about what AtlaZ can do? Visit us at booth #239 or get in touch! Discover more of our activities here: https://lnkd.in/dR6F2-D7 #FestivalOfBiologics #FoB2024 #CellAnalytics #AtlaZ #CellMonitoring #GPCRs #Immunology #Oncology #Virus

𝗛𝗼𝘄 𝗯𝗶𝗴 𝗶𝘀 𝘆𝗼𝘂𝗿 𝗡𝗮𝗻𝗶𝗼𝗻 𝗯𝗲𝗲𝗿 𝗺𝘂𝗴 𝗰𝗼𝗹𝗹𝗲𝗰𝘁𝗶𝗼𝗻? Since the start of our user meetings, we’ve kept a tradition alive: giving our participants custom Nanion beer mugs to take home. It is not just a mug, but a unique collectible that represents the spirit of each meeting. Some of our attendees have been collecting them for years—how many do you have? Even if you can’t make it to Munich this year, you don’t have to miss out! You can still join our user meeting online, experience all the science and be part of the discussions from the comfort of your office or home. Ready to join us this year? Join us virtually or in person and be part of this year’s meeting! 🍺 https://ow.ly/mXR050TyNUa We look forward to connecting with you! #CollectibleMugs #NUM2024 #Networking #ScientificReports #HybridMeeting #UserMeeting