Resilient Consulting

🔬 The intimate relationship between the epithelium and the immune system is essential for maintaining tissue #homeostasis. When this delicate balance is disrupted, it can lead to #autoimmunediseases and #cancer. While stem cell-derived #organoids have become powerful models for studying epithelial function, they fall short of capturing the complete organ-level processes due to the absence of tissue-resident immune cells. 🧫 Introducing human intestinal immuno-organoids (IIOs)! These groundbreaking models are formed through the self-organisation of epithelial organoids and autologous tissue-resident memory T (TRM) cells. A portion of these TRM cells integrate within the epithelium, continuously surveying the barrier. This interaction is driven by TRM cell-enriched transcriptomic programs that govern cell motility and adhesion, offering a deeper understanding of immune surveillance at the epithelial level. 🧬 By combining IIOs with single-cell transcriptomics, researchers (Timothy Recaldin et al.) investigated the complex dynamics of intestinal #inflammation triggered by cancer-targeting biologics in patients. The study uncovered that inflammation led to the emergence of an activated CD8+ T cell population, acquiring intraepithelial and cytotoxic features over time. This process was preceded by a T helper-1-like CD4+ population that initially produced cytokines and later became cytotoxic. 💡 The versatility of IIOs, coupled with their ability to mimic real immune interactions, allowed researchers to identify the Rho pathway as a potential target for mitigating immunotherapy-associated intestinal inflammation. These findings highlight the potential of IIOs in studying tissue-resident immune responses in #tumorigenesis, #infections, and autoimmune diseases. 🤔 How do you think this innovative approach with IIOs could impact future research in autoimmune diseases or cancer treatment? --- 👋 Hi, I’m Nia! I’m passionate about exploring the latest advancements in #immunology, #therapeutics, #biotech, and #startups. If you’re as fascinated by the future of #healthcare as I am, hit that follow button for more insights, discussions, and cutting-edge news. Let’s stay connected and keep the conversation going! #ScientificResearch #Immunology #Organoids #CancerResearch #AutoimmuneDiseases #Biotech #Innovation #HealthcareScience

🚀 #Spain’s Ascent: A Global Leader in #ClinicalTrials 🚀 🔬🌍 This achievement is driven by Spain's well-structured national healthcare system, which has enabled the country to participate in one out of every three clinical trials launched in #Europe. In 2021 alone, Spain accounted for 4.1% of global clinical trials, involving over 145,000 patients. The nation’s success in this field is also highlighted by its position as a top contributor to scientific publications in advanced #CellTherapies, surpassing countries like the #UK and #SouthKorea. 🏥💉 A significant factor behind Spain's leadership in clinical trials is its commitment to #collaboration across the #public and #private sectors. The Rovi-Insud Pharma alliance, part of the Vanguard Health Strategic Project for Economic Recovery and Transformation (PERTE), exemplifies this collaborative spirit. The BioRegion in #Catalonia, housing over 1,300 companies and 91 research institutions, further showcases how regional initiatives are driving innovation. Investors are increasingly attracted to #Spain, particularly in #Madrid and #Barcelona, where groundbreaking research, including Moderna’s mRNA vaccine trials, is taking place. 📈💡 Spain’s dedication to #PrecisionMedicine is another key aspect of its success. With #cancer research leading the way, precision medicine projects now make up nearly 30% of all studies in the country. This focus on personalised treatments has significantly grown, with #AdvancedTherapies gaining particular interest. Additionally, Spain is pushing for #decentralised clinical trials to ensure that #patients across the country have access to cutting-edge therapies, not just those in major cities. Networked trials are bringing investigational treatments to more people, demonstrating that collaboration is indeed the cornerstone of Spain’s research and #innovation. 💬 How do you see the role of collaboration in driving innovation in healthcare? ---    👋 Hi, I’m Nia! I’m passionate about exploring the latest advancements in #immunology, #therapeutics, #biotech, and #startups. If you’re as fascinated by the future of #healthcare as I am, hit that follow button for more insights, discussions, and cutting-edge news. Let’s stay connected and keep the conversation going! #ClinicalTrials #LifeSciences #Innovation #Healthcare #Spain #PrecisionMedicine #Biotech #Research https://lnkd.in/egUPmaUF

🧬 Unlocking Safe #Therapeutics: Gene Silencing with #Epigenetic Precision and Zinc Finger Technology 🧬 🔒 Gene silencing without the need for gene editing is paving the way for safer therapeutic applications. Researchers have developed an innovative strategy to repress multiple genes simultaneously using #ZincFinger proteins fused to Krüppel-Associated Box (KRAB) repression domains (ZF-Rs). This method offers a powerful tool for targeted gene modulation without altering the DNA sequence. 🦠 By optimising a lentiviral system for delivering ZF-Rs into hematopoietic cells, the authors (Davide Monteferrario, PhD et al.) from Sangamo Therapeutics, Inc., demonstrated that it's possible to multiplex up to three ZF-Rs, or even combine two ZF-Rs with a chimeric antigen receptor (#CAR). Impressively, the expression of these ZF-Rs had no negative impact on the integrity or functionality of the transduced cells, making this approach highly viable for therapeutic use. 📊 In both in vitro and in vivo experiments, ZF-R-expressing #Tcells achieved highly efficient gene repression over an extended period (up to 10 weeks). This repression was also linked to significant epigenetic remodeling, suggesting that ZF-Rs can effectively and sustainably modulate #gene expression without permanent genetic changes. 💡 To ensure #clinical safety, the researchers also developed methods to improve ZF-R #specificity, demonstrating a path toward the creation of ZF-Rs with a strong safety profile. This epigenetic-based approach to #CellEngineering could revolutionise gene therapy by offering a safer alternative to traditional #DNA editing techniques. 🤔 How do you think epigenetic gene silencing could transform the future of gene therapy and personalised medicine? ---    👋 Hi, I’m Nia! I’m passionate about exploring the latest advancements in #immunology, #therapeutics, #biotech, and #startups. If you’re as fascinated by the future of #healthcare as I am, hit that follow button for more insights, discussions, and cutting-edge news. Let’s stay connected and keep the conversation going! #GeneTherapy #Epigenetics #Biotech #ZincFingerProteins #HealthcareInnovation #GeneticResearch #PrecisionMedicine https://lnkd.in/eeatHuw3

🧽 We can’t wait to dive into testing the Lenti-X Transduction Sponge from Takara Bio Europe — an innovative tool that’s set to revolutionise our approach to #lentiviral transduction! 🧽 ✨ This cutting-edge sponge is set to transform the workflow by delivering incredible #TransductionEfficiency without the usual hassle—no need for #spinoculation or harsh chemicals. With the ability to transduce 10⁵–10⁷ cells effortlessly, we’re talking about a serious boost in #productivity and cell #viability. Whether it's standard cell lines or those notoriously difficult primary cells, the Lenti-X Transduction Sponge has got it covered! 💪 👩🔬 But that's not all—special thanks to Daniela Sordillo, PhD for bringing some humour to the lab with a #ScrubMommy, cleverly packaged like the Lenti-X Transduction Sponge! 😄 We might not use it in the same way, but it's sure to bring some smiles as we dive into our experiments. 🔬 Looking forward to putting this #novel technology to the test and seeing the results it brings! #Biotech #Innovation #LentiviralTransduction #LabLife #Grateful

🗝️ Unlocking the Secrets of the #Thymus: Technological Advances in T Cell Development and Immune #Tolerance 🗝️ 💡 The human #thymus, long a subject of scientific curiosity, plays a crucial role in T cell maturation and immune tolerance. Once misunderstood as a vestigial organ, our understanding of its complex functions has dramatically evolved since the groundbreaking work of Jacques Miller in 1961. The thymus is now recognized as essential for the development of #Tcells, with early T cell progenitors (ETPs) undergoing a series of intricate developmental steps within its structure. 🧪 Within the thymus, #thymocytes migrate from the outer cortex, where they undergo rapid proliferation and #PositiveSelection, to the inner medulla for #NegativeSelection. This process ensures that only T cells with appropriately regulated responses to self-antigens mature and enter circulation, while self-reactive T cells are eliminated or differentiated into regulatory T cells (#Tregs) to maintain immune tolerance. 🧩 Although these fundamental concepts have been established for decades, recent advancements in #technology have revolutionized our understanding of the thymus’s structure and function. Traditional methods like #histology, #immunohistochemistry, and #flowcytometry laid the groundwork, but they had limitations in #resolution and the #identification of diverse cell populations, especially within the stromal compartments. 🔬 Cutting-edge imaging and transcriptomics techniques, including high-resolution 3D imaging and single-cell #RNA sequencing, have provided unprecedented insights into the thymic #microenvironment. These advancements have revealed the heterogeneity of thymic cell populations, further elucidating the molecular mechanisms behind T cell development. Additionally, #organoid culture systems now allow for the ex vivo study of T cell and #stromal cell development, offering new opportunities to monitor thymic function and explore therapeutic strategies. 🌟 This review highlights the transformative impact of these technologies on our understanding of the thymus and underscores the potential for future research in this field. By harnessing these innovations, we can continue to unravel the complexities of thymic biology and develop novel treatments for immune-related disorders. 🤔 How do you think these technological advancements will shape the future of research and therapy in immune-related diseases? --- 👋 Hi, I’m Nia, a scientific leader with 10+ years of #immunology and #biotech experience! I’m passionate about exploring the latest advancements in #immunology, #therapeutics, #biotech, and #startups. 👍If you’re as fascinated by the future of #healthcare as I am, hit like and follow for more insights, discussions, and cutting-edge news. https://lnkd.in/ez6DwaN9 #TCellDevelopment #ImmuneTolerance #SingleCellOmics