Bioprinting Laboratories, Inc.

🔬 Revolutionizing Developmental Neurotoxicity (DNT) Testing with Dynamic Organoid Culture 🧠 Traditional in vitro cell models and animal studies have long been used to evaluate the neurotoxicity of chemicals. However, these methods often focus solely on acute toxicity or present physiological differences that limit their relevance to human brain development. Moreover, animal models are time-consuming, expensive, and ethically challenging. Enter human brain organoids—an emerging and promising alternative for assessing the effects of chemicals on the developing brain. Yet, conventional organoid culture systems come with challenges: low throughput, lack of reproducibility, limited maturity, and necrotic core formation due to poor nutrient and oxygen diffusion. Our pillar/perfusion plate technology is designed to overcome these limitations! By creating a dynamic culture environment with rapid, bidirectional flow, this innovative system enables the differentiation of cerebral organoids with enhanced proliferation, maturity, and viability. It’s an ideal solution for predictive DNT models and high-throughput compound screening, allowing for more accurate and ethical assessments of chemical toxicity. If you're working in DNT or neurotoxicity research, this is a game-changer. Let’s push the boundaries of what’s possible in brain organoid development! For more details, check out our publication: https://lnkd.in/gajvdSd3 #Organoids #Neuroscience #Toxicology #InVitroModels #DrugScreening #PerfusionCulture #Neurotoxicity #DevelopmentalNeurotoxicity #Innovation

🚀 High-throughput co-culture of spheroids for metabolism-mediated neurotoxicity testing 🧬 In the quest to better understand the complex interplay between chemicals and their metabolites, traditional in vitro neurotoxicity testing has often fallen short, particularly in replicating hepatic biotransformation. To address this critical gap, we’ve developed a cutting-edge, high-throughput testing system that integrates metabolically competent HepaRG cell spheroids with 3D-cultured ReNcell VM using our innovative 384PillarPlate. By combining HepaRG cell spheroids in an ultralow attachment (ULA) 384-well plate with ReNcell VM spheroids on our 384PillarPlate and generating compound metabolites in situ, we could assess the neurotoxic potential of parent compounds and their metabolites. This approach can be extended to any spheroid co-culture for investigating organ communications and performing high-throughput, high-content imaging assays by using spheroids. 📄 Read the full article: https://lnkd.in/gVrUggXt Looking forward to seeing how this technology will transform the landscape of drug safety testing! 🌟 👉 For more information, visit www.3dbpl.com #NeurotoxicityTesting #3DCellCulture #InVitroModels #Biotransformation #PharmaceuticalInnovation #ToxicologyResearch #HighThroughputScreening

One year ago, our article was published online: Title: A Pillar and Perfusion Plate Platform for Robust Human Organoid Culture and Analysis Journal: Advanced Healthcare Materials DOI: https://lnkd.in/gpYfiJN2 Since being published, our article has received 3288 downloads. 😊

🚀 Excited to share our latest innovation in human liver organoid culture! Traditional Matrigel dome cultures of organoids in 6-/24-well plates are often costly, with limited reproducibility and throughput. To address these challenges, we have developed an innovative microarray 3D bioprinting protocol utilizing a pillar plate and a deep well plate. By suspending foregut cells in Matrigel and bioprinting them onto our pillar plate, we have significantly scaled up liver organoid production. This was achieved by integrating the pillar plate with a 384-deep well plate, resulting in remarkable reproducibility (CV 15-18%) and enhanced maturity at a 10-fold reduced culture volume. The synergy between the pillar plate and the deep well plate has facilitated a robust and functional liver organoid culture, exhibiting superior viability, morphology, and essential liver functions, including albumin secretion and CYP3A4 activity. Furthermore, liver organoids cultivated on the pillar plate have been utilized in high-throughput hepatotoxicity assays with drugs such as sorafenib and tamoxifen. This scalable and reproducible method paves the way for advanced predictive compound assessments in clinical and pharmacological research. 🧫🔬 Read the article below: https://lnkd.in/g2yB3h9q For more information, visit: www.3dbpl.com #PillarPlate #LiverOrganoids #OrganoidCulture #HighThroughputScreening #BiomedicalInnovation #3DBioprinting

📣 Enhance Your 3D Cell Culture with Our Innovative Pillar/Perfusion Plate! Discover how our Pillar/Perfusion Plate revolutionizes dynamic 3D cell culture by eliminating necrotic core and enhancing cell growth, reproducibility, throughput, and user friendliness. This cutting-edge platform overcomes the limitations of traditional static 3D cell cultures, offering rapid diffusion and compatibility with common lab equipment. 🗝 Key Benefits: ↘ Reduced Necrotic Core and Improved Cell Growth: Rapid nutrient and oxygen diffusion. ⚡ High Throughput: Ideal for large-scale screenings. 🤝 User-Friendly: Easy integration with existing lab equipment. Read the full study in ACS Biomaterials Science & Engineering and elevate your research today! https://lnkd.in/epQFKMtz For more information, visit www.3dbpl.com

🚀 Revolutionizing Brain Organoid Culture for High-Throughput Screening 🧠 We’re thrilled to share our latest manuscript published in Biofabrication, where a simple and robust method of embryoid body (EB) transfer and encapsulation in Matrigel has been demonstrated on our pillar plate for cerebral organoid culture. This innovative method tackles some of the biggest challenges in brain organoid culture, such as low throughput, high variability, and labor-intensive processes. Key Highlights: 📌 Streamlined process: Eliminated the need for labor-intensive, multiple transfer, and encapsulation steps, making brain organoid culture more reliable and reproducible. 📌 High success rate: Achieved a 95% - 100% success rate of spheroid transfer to the pillar plate by a sandwiching and inverting method. 📌 Enhanced reproducibility in organoid culture: Achieved the coefficient of variation (CV) in brain organoid generation in the range of 9% - 19%, ensuring reliable results for your experiments. 📌 Miniaturized organoid culture: Reduced culture medium volume 10 times by using the pillar plate and significantly enhanced assay throughput by enabling in situ organoid testing, imaging, and analysis. This approach not only alleviates variability in organoid culture but also paves the way for high-throughput compound screening in clinical and industrial settings. If you are unsatisfied with large scale Matrigel dome culture and want to enhance the throughput of your organoid-based assays, this is a must-read! https://lnkd.in/g3CB29PW For more information, visit www.3dbpl.com #PillarPlate #PerfusionPlate #BrainOrganoid #OrganoidCulture #HighThroughputScreening #BiomedicalInnovation

🆓 Request your free samples today! 🆓 Dear researchers, are you: Struggling with non-reproducible organoids? Frustrated with increased cell death in the core and decreased organoid maturity? Tired of manually embedding your organoids with ECM Matrix one by one? Fed up with complicated systems involving pumps and tubes for your dynamic organoid culture needs? It’s time for a change. Increase reproducibility, throughput, and organoid maturity—all with one simple solution. Our pillar/perfusion plates and accessories are designed to simplify your organoid and 3D cell culture processes. Say goodbye to complex setups and hello to enhanced reproducibility, boosted throughput, and improved organoid maturity—without the need for pumps or tubes. DM Bioprinting Laboratories, Inc. or Pranav Joshi or Vanga Manav or email us at sales@3dbpl.com to request for free samples. Visit www.3dbpl.com for more information.

Human organoids hold immense potential for advancing drug screening and disease modeling. These miniature, multicellular structures mimic the architecture and functions of living tissues. However, challenges persist in achieving high-throughput screening (HTS) due to complex differentiation processes and scalability issues. A team of researchers from UNT College of Engineering, Cincinnati Children's and University of Illinois Chicago have tackled these obstacles by using specialized pillar and perfusion plates from Bioprinting Laboratories, Inc. These plates facilitate rapid stem cell printing and organoid differentiation, enhancing throughput and predictive accuracy. This innovation could revolutionize preclinical evaluations and environmental toxicant prioritization. Read more here: https://lnkd.in/g5mm2TGA For more information, visit www.3dbpl.com #Science #Biotechnology #Organoids #Research #Durgscreening #Diseasemodeling #Highthroughputscreening #3Dcellculture