Alamar Biosciences, Inc.’s Post

Establishing a convenient model of serum-induced reactivity in human astrocytes to investigate astrocyte-derived extracellular vesicles: Katherine White, Sebastien Serres at University of Nottingham and collaborators developed a human serum-free astrocyte culture system that maintains primary astrocytes in a quiescent state, enabling the study of morphology, function, and protein cargoes of astrocyte-derived EVs https://lnkd.in/gB5UNUGZ Proteomic analysis identified distinct protein profiles for both types of astrocyte-derived EVs, with enrichment of complement and coagulation cascades in EVs from chronically serum-cultured astrocytes, providing insights into their roles in the central nervous system. An article co-authored by Hannah Bailey, Barry S. Shaw, Philippine Geiszler, Raquel Mesquita-Ribeiro, Daniel Scott and Robert Layfield #extracellularvesicles #exosomes #proteomics #astrocytes #serum #Vesiculab

Very happy and excited to share our paper published in Science Magazine yesterday. Here, we used state of the art techniques to understand the formation, structure and repair of replication associated DNA breaks. Highlights: - Leading and Lagging strand breaks can have different structures - Homologous recombination (HR) is the primary pathway to repair collapsed forks - One ended double strand breaks (DSBs) are converted to two-ended DSBs by converging replication forks in HR-deficient cells, creating a substrate for mutagenic end joining reactions - BRCA1 deficiency does not impact resection at collapsed forks. The main role of BRCA1 in this context is to recruit RAD51 to promote HR - 53BP1 counteracts RAD51 loading at resected replication breaks rather than impairing resection A huge thanks to andre nussenzweig, Veenu Tripathi, all the members of the Nussenzweig lab and all the terrific collaborators that made this work possible Kyle Vrtis, Johannes Walter, Giordano Reginato, Petr Cejka, Wei Wu

🌟𝐄𝐱𝐜𝐢𝐭𝐢𝐧𝐠 𝐃𝐢𝐬𝐜𝐨𝐯𝐞𝐫𝐲 𝐀𝐥𝐞𝐫𝐭!🌟 🔬 A recent study uncovered a fascinating link between Apelin signaling and sprouting angiogenesis. By identifying Apelin-expressing neural progenitor cells in the dorsal neural tube, researchers revealed how Apelin guides tip cell migration and influences key behaviors. This research sheds light on the intricate interplay between neural and vascular systems during blood vessel formation, offering valuable insights for potential therapeutic strategies. #ResearchDiscovery #ApelinSignaling #Angiogenesis 🚀💡 Link to this study in the comment below. If you are attending #ISSCR2024, drop by Sino Biological, Inc. at booth 602! Let's talk. 😊

Implementing confocal spectroscopy to assess payload heterogeneity in lipid nanoparticles: Yizong Hu, Tine Curk, Hai-Quan Mao, Jeff Tza-Huei Wang at The Johns Hopkins University and collaborators introduced cylindrical illumination confocal spectroscopy combined with in-line single-nanoparticle free solution hydrodynamic separation to simultaneously characterise the size of RNA-LNPs and the loading levels of RNA, helper lipids, and PEGylated lipids at the single-nanoparticle level https://lnkd.in/eXendpEt They observed that correlating LNP size with payload provided insights into LNP structure and RNA distribution, revealing significant heterogeneity in payload distribution even among LNPs of the same size. An article also authored by Sixuan Li, Jinghan Lin, Zachary Schneiderman, Fangchi Shao, Lai Wei, Andrew Li, Kuangwen Hsieh and Efrosini Kokkoli #LNP #nanoparticles #spectroscopy #drugdelivery #payload #Vesiculab

Join us Feb. 28 to explore recent #spatial #multiomics trends and how to deepen biological insights.

As a neuroscientist myself, I'm very excited for this one. By mapping gene expression in brain tissues, spatial transcriptomics offers unparalleled insights into the cellular organization and molecular complexity of the brain, pinpointing exact locations of disease-related gene expression changes, and providing a holistic view of cellular interactions and functions. We're excited to join Curio Biosciences on March 21 to discuss how their spatial mapping kits, coupled with our analysis pipelines and research environment, enhances brain-related research and how they've helped to reveal the role of a schizophrenia-associated microRNA in the cerebellum. #SpatialTranscriptomics #neurologicaldisorders #schizophrenia #Diagnostics #Innovation #Biotechnology If you'd like to join as well you can register here: https://hubs.ly/Q02nCp240

🥚✨𝙊𝙪𝙧 𝙡𝙖𝙩𝙚𝙨𝙩 𝙖𝙧𝙩𝙞𝙘𝙡𝙚 𝙝𝙖𝙨 𝙟𝙪𝙨𝙩 𝙗𝙚𝙚𝙣 𝙧𝙚𝙡𝙚𝙖𝙨𝙚𝙙! 🥚✨ Our latest research uncovers some hidden connections between follicular environment parameters and the quality of mature oocytes. Discover the results of our hard work, utilizing tools like NGS, time-lapse imaging, and cell staining to study oocyte quality. Our findings, published in JARG, provide new insights into oocyte quality. 🧬🔬 #ReproductiveScience #OocyteQuality #NGS #TimeLapseImaging #CellStaining #JARG #NewResearch 🚀 Link: https://lnkd.in/d5zT3QAs Judy Tanios Marwa Bazzi ZALIHE YARKINER Munevver Serdarogullari Georgios Liperis, PhD youmna Mourad Dr. Chadi Fakih

Thrilled to share this preprint where we propose a new approach to derive molecular connectivity from dynamic PET scans (using another tracer than FDG, in this case 11C-DASB), and investigate whether the extracted readouts offer additional value in the context of a pharmacological challenge (in this case MDMA) when compared to (a) effects on "classic" fMRI-derived functional connectivity and (b) changes in PET binding potential. Check it out to see what we found out!

In this recent paper from Nature, Researchers at the Arc Institute and The University of Tokyo have unveiled a novel mechanism for precise genomic rearrangements using bridge RNAs (bRNAs). Learn how Monolith was used in this groundbreaking article to measure binding affinities of IS621 RNP for donor and target DNA, as well as for donor and target DNA containing scrambled LD–RD and LT–RT regions. Read the full publication: https://lnkd.in/gZ_GM3i5

Imagine seeing every molecule in a cell simultaneously! Yale's FLASH-PAINT, an evolution of DNA-PAINT, does just that.  It's faster, more detailed, and cost-effective, paving the way for new discoveries in #SpatialBiology. Explore how this cutting-edge technique helps map protein interactions and reveals intricate cellular processes, potentially transforming disease research and diagnostics. #Microscopy #SuperResolution #DNAPAINT