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Axion BioSystems

10,094 followers

7mo Edited

In this example, Marc Davies, PhD at Leucid Bio shows how the Omni Pro 12 captures the kinetic response of CAR T cells during re-stimulation assays—highlighting how real-time, continuous data offers deeper insights compared to conventional endpoint assays. To learn more about how regular monitoring of your assay with the Omni Pro 12 can elevate your research, be sure to watch our on-demand webinar via 🔗 https://bit.ly/3OMNwuW #CellTherapy #ImmunoOncology #OmniPro12 #LiveCellImaging

Transcript

We then turned to the Omni Pro from axion to look at the kinetics behind this. A lot of the initial stuff was done with endpoint assays, which are very important because they show whether your T cells are functional, but you can combine that with kinetic kinetic data you get from more regular monitoring, which. Machines such as the Omni Pro allow to look at the kinetics behind these RE stimulations and here you can see this is just looking at 2 rounds of stimulation. You can see at 72 hours we added some fresh tumor in. So the T cells have managed to lose most of the tumor. We added fresh tumor in at 72 hours and you can see that the reduction in tumor viability is much quicker the second time round as a result of the fact that these T cells had nicely proliferated. As you can see from the blue line, the entrance educed T cells had no efficacy. Effect upon the tumor cell lysis.

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