BIOREMIA ETN’s Post

🎉 The amazing NIS team has resolved four structures of HiTehA with cryo-EM! 🔬 They demonstrate that #cryoEM can effectively analyze fully embedded membrane proteins without the use of fiducial markers, an important step forward in studying membrane proteins. 👨🔬 Structural analysis of #membraneproteins is often challenging due to low protein yields and their unstable nature. #HiTehA is particularly challenging due to its encapsulation in detergent micelles, which obstructs proper alignment of projections needed for #3Dreconstruction. 📑 Check out the paper to see the details:

🎉 The amazing NIS team has resolved four structures of HiTehA with cryo-EM! 🔬 They demonstrate that #cryoEM can effectively analyze fully embedded membrane proteins without the use of fiducial markers, an important step forward in studying membrane proteins. 👨🔬 Structural analysis of #membraneproteins is often challenging due to low protein yields and their unstable nature. #HiTehA is particularly challenging due to its encapsulation in detergent micelles, which obstructs proper alignment of projections needed for #3Dreconstruction. 📑 Check out the paper to see the details:

🎉 The amazing NIS team has resolved four structures of HiTehA with cryo-EM! 🔬 They demonstrate that #cryoEM can effectively analyze fully embedded membrane proteins without the use of fiducial markers, an important step forward in studying membrane proteins. 👨🔬 Structural analysis of #membraneproteins is often challenging due to low protein yields and their unstable nature. #HiTehA is particularly challenging due to its encapsulation in detergent micelles, which obstructs proper alignment of projections needed for #3Dreconstruction. 📑 Check out the paper to see the details: https://lnkd.in/gxw4zBWV

Developed in collaboration with the MacMillan lab, Dione and Hyas kits allow users to rapidly conjugate iridium catalysts onto any small molecule. The kits allow for facile incorporation of small molecules as an amide linkage, either with an amine or acid group; have a short labeling radius; use a benign method of activation (visible light); and ensure catalytic signal amplification. Discover how μMap Photoproximity Labeling can advance your #DrugDiscovery research! https://bit.ly/4cGZrnP . #TeamMilliporeSigma

Developed in collaboration with the MacMillan lab, Dione and Hyas kits allow users to rapidly conjugate iridium catalysts onto any small molecule. The kits allow for facile incorporation of small molecules as an amide linkage, either with an amine or acid group; have a short labeling radius; use a benign method of activation (visible light); and ensure catalytic signal amplification. Discover how μMap Photoproximity Labeling can advance your #DrugDiscovery research! https://bit.ly/4cGZrnP . #TeamMilliporeSigma

New publication alert! Many congrats to Yuchen Zhu and Jiaxin Hou on getting this fundamental study on the cation dependent aggregation behaviour of PNIPAM nanogels. It is also our first collaboration work with Tom McDonald. While the thermal stimulation of the colloidal PNIPAM leading to reversible aggregation and redispersion is very intriguing, this work highlights the contribution of electrolytes in the media to this behaviour and defines the limits where the aggregation process becomes irreversible. https://lnkd.in/edKvGGmy

When was the last time you screen compound via SAXS? Kudos for adding some new color. The gap 📣 Traditional SAR has been generating large dataset from a 2 dimensional matrix, cellular phenotypical readout and dynamic interaction based readout. Not a tons of attention had been shifted towards macromolecular conformation. With a lot of efforts done towards finding allosteric binding site and/or understand MoA to de-risk single-target-multiple-chemotype HTS campaign, below described TR-HT-SAXS approach bears potential in unlocking key information in the early stage of SAR efforts. The approach 🚿 This team combines AIF as target and rapid SAXS to measure rates of monomer-dimer transition ((kVR) in an effort to screen 2500 fragment compound library. The data revealed a subset of chemotype that promotes strong dimerization of AIF macromolecule. This team also provides an easy to adopt x-ray workflow. Technique 🔭 SAXS is a rapid techniques that monitors macromolecule solution architectures. Recent advance in sample-handling and synchrotron beamline process speed increase throughput and accessibility of SAXS in rapid early stage chemotype triage. Implication 💪 This approach provides kinetics insights for compound induced molecular conformational state. It expands the scope of early SAR efforts in differentiating compound scaffolds and understanding of Mode of Interaction. Furthermore, with recent interest in analyzing multiprotein complexes. In conjugation with other biophysical methods. #drugdiscovery #xraycrystallography #cryoem #microed #SAXS #allostericinhibition https://lnkd.in/g93wRMgj

Check out our recent study on efficiently coupling various dyes onto peptides. It had some surprising outcomes highlighting the importance of almost forgotten chemistry knowledge. Although the study was limited to ATCUN peptides, we strongly believe it has some general applicability!

Developed in collaboration with the MacMillan lab, Dione and Hyas kits allow users to rapidly conjugate iridium catalysts onto any small molecule. The kits allow for facile incorporation of small molecules as an amide linkage, either with an amine or acid group; have a short labeling radius; use a benign method of activation (visible light); and ensure catalytic signal amplification. Discover how μMap Photoproximity Labeling can advance your #DrugDiscovery research! https://bit.ly/4cGZrnP . #TeamMilliporeSigma

Molecular dynamics…..