Bradley Droubay, MBA’s Post

𝗩𝗲𝘀𝗶𝗰𝗹𝗲 𝗣𝗿𝗲𝗽 𝗣𝗿𝗼 - 𝗟𝗶𝗽𝗼𝘀𝗼𝗺𝗲𝘀 𝗺𝗮𝗱𝗲 𝗲𝗮𝘀𝘆 🫧 The Vesicle Prep Pro offers a standardized and robust way to reliably and reproducibly generate GUVs of homogeneous size distribution with high yields. It is the first product on the market for the automated preparation of solvent-free GUVs, and it has been designed to keep the preparation process simple and ensure stable and reproducible results. With integrated features like flexible protocol design and temperature control, you can generate GUVs from a wide variety of lipids, including lipids with high charge or high phase transition temperatures. GUVs prepared with the 𝗩𝗲𝘀𝗶𝗰𝗹𝗲 𝗣𝗿𝗲𝗽 𝗣𝗿𝗼 have proven ideal for investigations of biophysical properties of e.g., electrogenic transmembrane proteins, lipid raft formation, and protein-DNA interactions. Do you want to learn more about Vesicle Prep Pro capabilities? Read more here: https://ow.ly/iyt350RJVG1 #Biophysics #MembraneBiophysics #Lipids #Vesicles #GUVs #GiantUnilamellarVesicles #VPP #VesiclePrepPro

Formation of #biofilms containing #microbial species, for example on medical devices, can be problematic. With help from the LUNA-FL Dual #Fluorescence #CellCounter, scientists developed an anti-quorum sensing coating that prevents microbial cell-to-cell communication, reducing biofilm formation. https://ow.ly/MiU850QqqNk #cellcount #cellcounting #biofilm #microbe #quorumsensing #electrospinning

Newly launched! EasyFLO full spectrum flow cytometer!   EXPEC grandly launched the EasyFLO full-spectrum flow cytometer, which is specially designed to meet the needs of routine testing in laboratories and greatly improve process detection capabilities. EasyFLO integrates several innovative technologies to bring a more accurate, efficient, and convenient flow cytometry analysis experience.   Highlights: 1. Innovative technology: Integrating advanced technologies such as innovative achromatic shaping optical path, all-optical harmonic synchronous acquisition, and array sensor high-speed acquisition, greatly improves detection capabilities and efficiency. 2. High precision: Supports high-precision injection pump sampling and high-stability sample focusing capabilities based on the pulsation-free sheath flow system to ensure the accuracy of experimental data. 3. Multi-parameter analysis: Combining the CytoPysual flow analysis workstation and powerful software algorithms, it avoids the compensation problem caused by fluorescence spectrum overlap, realizes single laser 15-channel 10-color multi-parameter analysis, and meets 80% of analysis needs. 4. Easy to use: Simple to operate and easy to use, reducing training costs and time.   EasyFLO full spectrum flow cytometer is mainly used in the life science field. Ask EXPEC today to learn more about the EasyFLO full-spectrum flow cytometer! #analyze #analysis #analyzer #labinstruments #testequipment #biotechnologies #environmentalmonitoring #spectrometer #spectroscopy #environment #testing #laboratory #instruments #flowcytometry #cellanalysis #apoptosis #lifesciences #blooddisease #tumor #Unicellular #immunology #drugresearch #medicine #cellbiology #stemcells  #FLO

A significant challenge in cellular research is the creation of a clonal cell population and the precise counting and expanding of these cells within the same workflow. Reliable tools that support your workflow are essential to achieving excellence in clonal analysis and expansion.   The OLS 3D workflow allows going from single cells to monoclonal organoids. Cell Isolation, Counting, Culture and Assay and Readout are done in an integrated approach offering high standardization, reproducibility and reduced consumables and substrates.   Check out how you can transform your cellular research by using the CASY Cell Counter and Analyzer, CERO 3D Incubator and Bioreactor and the CellRaft AIR technology.   https://lnkd.in/eg_Rkffb   #3Dcellculture #iPSC #Organoids #DrugDiscovery #PersonalizedMedicine #Diseasemodeling

PROTACs: Navigating the Labyrinth of Protein-Ligand Interactions Understanding the intricate details of protein-ligand interactions is crucial for PROTAC development. Traditional methods like crystallography and Cryo-EM can be cumbersome, expensive, and low throughput. At NeoProteomics, our groundbreaking HRF technology has helped unleash discovery across a broad range of therapeutic targets, particularly in the autophagy pathway. Our approach allows us to label virtually the entire protein surface under physiological conditions, and provide deep mechanistic insights into conformational epitopes. Reach out to us today for a free consultation on how HRF can accelerate your PROTAC development! #PROTAC #structuralbiology #proteinfootprinting #epitopemapping #drugdiscovery #drugIP

New England Biolabs has introduced the NEBNext Enzymatic 5hmC-seq Kit (E5hmC-seq), an innovative enzyme-driven technique tailored for precise identification of 5hmC sites. This method promises exceptional data quality and yield, accommodating input amounts ranging from 100 picograms to 200 nanograms. Comprehensive in scope, the kit encompasses all necessary reagents for E5hmC-seq conversion and library preparation, seamlessly compatible with Illumina sequencing platforms. Index primers for multiplexing purposes can be procured separately. Additionally, an optional conversion module extends its utility for diverse applications beyond conventional library preparation workflows. #dnaanalysis #enzymaticmethod #epigenetics #neb #illumina #5hmC-seq

🔬 Happy #MicroscopyMonday!     Beautiful data images are just one of the many rewards for studying #tumoroids using the Pu·MA System. This 4IC tumoroid was formed in #VitroGel from TheWell Bioscience, treated with Vimentin, Hoechst, and Phalloidin, and imaged with Yokogawa CQ1, all in our #flowchip.    Learn more about this simple workflow: https://lnkd.in/g-fxzn9U 

Excited to share our latest paper in Advanced functional materials, from the Sathish Ramakrishnan lab, on a rapid way to isolate highly pure #extracellularvesicles using photosensitive nanoprobes. #exosomes Why focus on size specific sorting of #EVs? Different sizes often mean different roles in cellular origin and even disease, yet isolating them while keeping them in their native form, free from non-vesicular particles, has been a challenge... until now! Major Finding #1: Our photosensitive nanoprobes can isolate EVs directly from complex biological media in under an hour, achieving high purity and efficiency. Our method provides a rapid, scalable solution for EV isolation Major Finding #2: We've devised a simple way for size-specific isolation with our approach. This allows us to enrich specific EV and synthetic vesicle subpopulations and retain them in their near native form. #sizesorting Major Finding #3: Our method outperforms traditional antibody-based techniques by capturing the entire EV population across various cell lines. Unlike methods that target specific markers and miss relevant EVs, we ensure no vesicle is left behind. #ComprehensiveCapture Major Finding #4: In our #Alzheimers model using N2a cells, we underscore the necessity of isolating the full spectrum of EVs for accurate biomarker analysis. Protein biomarkers predominantly found in a unique EV subpopulation and only contained minimal exosomal tetraspanin markers. A massive thanks to our dedicated team and collaborators Roshan Gunasekara, Ph.D. Abhijith Radhakrishnan Mackenzie Topper for their hard work. This is a milestone in #EVisolation strategies and it's just the beginning. https://lnkd.in/eK5ZYukj

📢 Recently published in Cytotherapy: Researchers at Texas A&M University demonstrate a scalable method for culturing #iPSC-derived ihMSCs using customizable gelatin methacryloyl (GelMA) microcarriers in 3L Vertical-Wheel Bioreactors. This cost-effective approach yields ihMSCs with superior bone regenerative and immunosuppressive properties compared to traditional monolayer cultures. "Vertical-Wheel bioreactors offer the same flexibility and scalability as stirred-tank bioreactors, but subject cell-laden microcarriers to less shear stress, representing a suitable compromise between scalability and agitation parameters for large-scale cell manufacture on microcarriers." Read more about this innovative and effective approach here: https://lnkd.in/ecwntAUu #celltherapy #MSCs #scaleup #scaledown

Why is TEM the method of choice for Adenoviral Vector characterization?  Beyond qualitative observations, TEM enables quantitative analysis of Adenoviral Vectors. At QuTEM AB, we can measure key parameters such as particle size, intergrity and aggregation analysis, and surface morphology, providing valuable data for optimizing vector design and formulation.    In the field of Adenoviral Vector production, ensuring consistency and quality is paramount. TEM serves as a valuable tool for validation and quality control, allowing researchers to assess batch-to-batch variability, detect potential defects or contaminants, and optimize production processes for maximum efficacy and safety.   #Adenoviruses #GeneTherapy #Biotechnology #QuTEM #TEM