Nanion Technologies

𝗖𝘂𝗿𝗶𝗼𝘂𝘀 𝗮𝗯𝗼𝘂𝘁 𝘁𝗵𝗲 𝗹𝗮𝘁𝗲𝘀𝘁 𝗶𝗻𝘀𝗶𝗴𝗵𝘁𝘀 𝗶𝗻 𝗰𝗮𝗿𝗱𝗶𝗮𝗰 𝘀𝗮𝗳𝗲𝘁𝘆? Join us at the Cardiac Safety Symposium 2025 for an exciting opportunity to connect with experts and explore cutting-edge research in safety pharmacology, cardiac physiology, and electrophysiology. 🗓 When: May 21, 2025 🌍 Where: Virtual symposium Why attend? ✔️ Stay ahead of the curve with insights on preventing drug-induced cardiotoxicity using the latest safety testing methods. ✔️ Learn from the HESI Stem Cell Working Group about drug effects on stem cell-derived and native cardiomyocytes. ✔️ Dive deep into cardiac ion channel studies and discover their critical role in heart function and drug safety. This event is ideal scientists and professionals in drug development, safety pharmacology, and cardiac research, offering a platform for knowledge-sharing and interactive discussions. Don't miss out! Register today and secure your spot: https://ow.ly/VvWl50TPkbR #CardiacSafety #SafetyPharmacology #Pharmacology #DrugDevelopment #CardiacResearch #Symposium2025

𝗪𝗲𝗯𝗶𝗻𝗮𝗿: 𝗥𝗲𝗹𝗶𝗮𝗯𝗹𝗲 𝗔𝗰𝘁𝗶𝘃𝗮𝘁𝗶𝗼𝗻 𝗼𝗳 𝗻𝗔𝗖𝗵𝗥 α𝟳 – 𝗦𝗽𝗲𝗲𝗱, 𝗣𝗿𝗲𝗰𝗶𝘀𝗶𝗼𝗻, 𝗮𝗻𝗱 𝗛𝗶𝗴𝗵 𝗧𝗵𝗿𝗼𝘂𝗴𝗵𝗽𝘂𝘁! The α7 subtype of nicotinic acetylcholine receptors (nAChR α7) is vital for neurological and immune signaling and shows promise for treating Alzheimer’s, schizophrenia, and other disorders. However, nAChR α7 studies face challenges like rapid desensitization and low conductance. 📅 When: November 21, 2024 at 16:00 h CEST Our webinar will showcase: ✔️ 𝗔𝗱𝘃𝗮𝗻𝗰𝗲𝘀 𝗶𝗻 𝘀𝘁𝗮𝗯𝗹𝗲 𝗰𝗲𝗹𝗹 𝗹𝗶𝗻𝗲𝘀: Learn about the Eurofins DiscoverX nAChR α7/ric3 HEK cell line. This line enhances receptor expression, achieving reliable acetylcholine response with positive allosteric modulators (PAMs). ✔️ 𝗩𝗮𝗹𝗶𝗱𝗮𝘁𝗶𝗼𝗻 𝗼𝗳 𝗥𝗲𝗮𝗱𝘆-𝘁𝗼-𝗔𝘀𝘀𝗮𝘆 (𝗥𝗧𝗔) 𝗳𝗿𝗼𝘇𝗲𝗻 𝗰𝗲𝗹𝗹𝘀: These cells eliminate the need for live cultures and deliver consistent results post-thaw. Validation data will highlight successful acetylcholine potentiation with PAMs like PNU-120596 and NS-1738 on SyncroPatch 384. ✔️ 𝗡𝗲𝘄 𝗡𝗣𝗖-𝟯𝟴𝟰𝗧 𝗙/𝗠 𝗦𝗧𝗜𝗠 𝗖𝗵𝗶𝗽: Nanion’s F/M STIM chips deliver superior performance with higher seal resistance, greater current amplitudes, and faster response kinetics, while maintaining consistency in EC50 values for ligands such as acetylcholine, nicotine, and the PAM PNU-120596. Join us at the webinar to get insights from James Costantin (Eurofins DiscoverX) on cell line advancements, and from Ilka Rinke (Nanion Technologies) on enhanced capabilities of the NPC-384T F/M STIM chip on the SyncroPatch 384. 📅 Save your spot and register here: https://lnkd.in/dzpxH4cT #Webinar #Neuroscience #IonChannels #Electrophysiology #DrugDiscovery #APC #SyncroPatch384 #PatchClamp

𝗔𝗰𝗰𝗲𝗹𝗲𝗿𝗮𝘁𝗶𝗻𝗴 𝗶𝗼𝗻 𝗰𝗵𝗮𝗻𝗻𝗲𝗹 𝗿𝗲𝘀𝗲𝗮𝗿𝗰𝗵: 𝗥𝗮𝗽𝗶𝗱 𝗻𝗔𝗖𝗵𝗥 α𝟳 𝗮𝗰𝘁𝗶𝘃𝗮𝘁𝗶𝗼𝗻 𝗼𝗻 𝘁𝗵𝗲 𝗦𝘆𝗻𝗰𝗿𝗼𝗣𝗮𝘁𝗰𝗵 𝟯𝟴𝟰 Nicotinic acetylcholine receptors (nAChRs), particularly those containing the α7 subunit, play a crucial role in neural pathways related to cognition, making them promising targets for the treatment of Alzheimer's and schizophrenia. These receptors respond to acetylcholine and nicotine but desensitize very rapidly, providing challenges for experiments involving these targets. The SyncroPatch 384 offers a breakthrough in studying nAChR α7 thanks to its flexible pipetting parameters (e.g. speed of application and exposure time) coupled with the new 𝗡𝗣𝗖-𝟯𝟴𝟰 𝗙/𝗠 𝗦𝗧𝗜𝗠 𝗰𝗵𝗶𝗽𝘀, ensuring: ✔️ Accurate recordings of fast-desensitizing ligand-gated ion channels. ✔️ Rapid ligand application, short exposure times and complete washout, minimizing receptor desensitization. ✔️ Precise patch-clamp aperture alignment with the pipette for optimal accuracy. ✔️ Flexible exposure settings with customizable ligand volume, application speed, and wait time. The SyncroPatch 384 enables the acquisition of consistent, high-quality data for α7 receptors and other fast-desensitizing ion channels. 📄 Want to see how SyncroPatch 384 achieves this? Download the flyer here: https://ow.ly/NTep50TUcZu #IonChannelResearch #Electrophysiology #PatchClamp #AlzheimersResearch #SynapticFunction #nAChRalpha7 #Neuroscience

The 70 kDa Heat Shock Protein (Hsp70) chaperones are critical components of the cell’s protein quality control system, playing key roles in protein folding, translocation, and disaggregation. Proteins synthesized in the cytoplasm rely on Hsp70 chaperones to move through specialized membrane channels/pores into organelles. Despite their well-established functions, the exact physical mechanism by which Hsp70 exerts force on its substrates has remained unresolved. In a recent study published in Nature Communications, researchers employed nanopore technology and electrophysiology recordings to directly observe the mechanism of Hsp70-mediated pulling at the single-molecule level. Their findings provide the first definitive evidence for the “Entropic Pulling” model, which posits that Hsp70 generates force by reducing entropy when it binds to a substrate emerging from a translocation channel. This entropic reduction prompts the chaperone to move away from the channel, pulling the bound protein with it. Using the Orbit mini platform for electrophysiology recordings, the team tracked ionic currents through nanopores embedded in artificial lipid membranes. This setup allowed them to monitor the escape of polypeptide substrates under the influence of Hsp70, providing real-time measurements of the chaperone’s pulling force. Remarkably, the researchers observed that Hsp70 could generate forces as strong as 46 pN over a distance of 1 nm, sufficient to extract polypeptides from the nanopore. The force was dependent on the size of Hsp70, in line with the Entropic Pulling model’s predictions, and distinct from other models such as the Power Stroke or Brownian Ratchet. Furthermore, molecular dynamics simulations supported these findings, revealing that the pulling force scales with the size of the protein domain. The study also showed that this entropic mechanism allows Hsp70 to perform tasks like protein translocation and aggregate disassembly, suggesting a broader application of Entropic Pulling in various cellular processes. In conclusion, this study establishes Hsp70 as a molecular motor that harnesses entropy to generate force, with implications for cellular protein homeostasis and potential therapeutic interventions in diseases related to protein misfolding and aggregation. The integration of nanopore technology and electrophysiology recordings in this research offers a powerful tool for future investigations into molecular chaperone mechanics. -- Find the full article here: https://lnkd.in/gPr_8vrh Discover how parallel lipid bilayer recordings can be utilized to study nanopores: https://lnkd.in/gKwdhdA9 #hsp70 #electrophysiology #ionchannels #lipidbilayer #nanopore 

LYCHOS (LYsosomal CHOlesterol Signalling) is an important human protein located in the lysosomal membrane and acting as a cholesterol sensor. LYCHOS transduces cholesterol levels into activation of mTORC1 signalling, thereby regulating cellular metabolism and growth. Despite its significance, the structural basis of how LYCHOS assembles and operates has remained elusive. In a recent study published in Nature, researchers employed high-resolution cryo-EM to elucidate the structure of LYCHOS, revealing its unique assembly composed of a transporter-like domain fused to a GPCR domain. Both the GPCR and transporter domains work together to coordinate cholesterol sensing through a conserved cholesterol-binding motif located at the interface of these domains. Surprisingly, the researchers found that the LYCHOS transporter-like domain is actually an orthologue of the plant PIN auxin transporter family, with a conserved crossover motif that enables a characteristic elevator-type conformational change. Analysis suggests a model in which the transporter domain's elevator motion, similar to that observed in plant PIN transporters, facilitates cholesterol entry by repositioning blocking residues. Moreover, surface plasmon resonance analysis determined that LYCHOS retained the ability to bind indole-3-acetic acid (IAA), the main auxin produced by plants, although with a lower affinity compared to plant PINs. Consistent with this, solid-supported membrane (SSM)-based electrophysiology measurements of LYCHOS revealed that an order-of-magnitude higher concentration of IAA was required to elicit electrogenic currents equivalent to those initiated by PIN8 in plants. The authors concluded that these findings are consistent with anion binding but likely not with transport across the bilayer. Importantly, mutations disrupting the cholesterol-binding mechanism significantly altered mTORC1 activity, underscoring the importance of LYCHOS in integrating cellular cholesterol levels with growth signalling pathways. In conclusion, this study presents LYCHOS as an unusual example of a GPCR that functions as a component within a larger transmembrane assembly, also featuring a PIN-like transporter domain. This discovery classifies LYCHOS as a unique GPCR-transporter hybrid, potentially opening new avenues for modulating mTORC1 signalling in the treatment of diseases. -- Find the full article here: https://lnkd.in/eCASgw27 Are you also studying organellar transporters? Have you considered using SSM-based electrophysiology? You can learn more about it here: https://lnkd.in/etrZ_kRZ #transporters #electrophysiology #ssm #lysosome #gpcr

And here we are – our 𝗡𝗮𝗻𝗶𝗼𝗻 𝗨𝘀𝗲𝗿 𝗠𝗲𝗲𝘁𝗶𝗻𝗴 𝟮𝟬𝟮𝟰 has come to a close! It’s been two great days of science, discussions, and collaboration. Today started with Stefan Kubick’s talk on cell-free systems for membrane protein production, followed by 𝗚𝗿𝗲𝗴𝗼𝗿 𝗔𝗻𝗱𝗲𝗿𝗹𝘂𝗵, who spoke about pore-forming toxins and nanosensing. Tobias Ensslen then shared interesting insights into real-time peptide differentiation using nanopore technology. We also had an enlightening cardiac research session. Jamie Bhagwan discussed the chamber-specific responses of hiPSC-derived cardiomyocytes, while Jieun An demonstrated how Cardiosight®-S with CardioExcyte96 can be used for cardiotoxicity screening. Jamie Vandenberg rounded out this session with his work on high-throughput phenotyping of cardiac ion channel variants. In the afternoon, Janina Sörmann explained how machine learning tools can be used to design hASIC1a modulators, and 𝗥𝗮𝗷𝗻𝗶𝘀𝗵 𝗥𝗮𝗻𝗷𝗮𝗻 introduced the CHANNELOME platform, which could bring new ideas to drug screening. 𝗞𝗮𝗿𝗲𝗻 𝗘𝗹𝘃𝗲𝗿𝘀 and 𝗜𝘄𝗮𝗻 𝗪𝗶𝗹𝗹𝗶𝗮𝗺𝘀 talked about using the SyncroPatch 384 in drug discovery, and Alexandr Ilyaskin provided a structure-based analysis of ENaC activation. Ali Obergrussberger wrapped up the meeting with closing remarks, summing up two days of inspiring science and collaboration. A big thank you to everyone who worked behind the scenes to make this meeting possible – it was truly a team effort! Special thanks to Yeimar Portillo and Ulrich Thomas for their dedication and hard work in making everything run smoothly. And of course, 𝘁𝗵𝗮𝗻𝗸 𝘆𝗼𝘂 to everyone who participated, both online and in-person. We were delighted to host you and look forward to seeing you again next year! #NanionUserMeeting #Electrophysiology #APC #MembraneBiophysics #CellAnalytics

𝗥𝗲𝗮𝗱𝘆 𝘁𝗼 𝗱𝗶𝘀𝗰𝗼𝘃𝗲𝗿 𝗻𝗲𝘄 𝗶𝗻𝘀𝗶𝗴𝗵𝘁𝘀 𝗶𝗻𝘁𝗼 𝘁𝗿𝗮𝗻𝘀𝗺𝗲𝗺𝗯𝗿𝗮𝗻𝗲 𝘁𝗿𝗮𝗻𝘀𝗽𝗼𝗿𝘁𝗲𝗿𝘀? 𝗝𝗼𝗶𝗻 𝘂𝘀 𝗮𝘁 𝗜𝗧𝗧𝗦 𝟮𝟬𝟮𝟰! Meet the Nanion team at the International Transmembrane Transporter Society (ITTS) Meeting in Bethesda, Maryland! This is your chance to connect with Rocco Zerlotti (Application Scientist), Cecilia George (Senior Sales Manager SURFE2R / Senior Scientist), and Federico Amodeo (Field Application Scientist) to learn more about our Solid-Supported Membrane-based Electrophysiology systems to investigate membrane transporters. Event details: 🗓️ When: October 29 – November 1, 2024 📍 Where: NIH Campus, Bethesda, Maryland 𝗗𝗼𝗻'𝘁 𝗺𝗶𝘀𝘀 𝗼𝘂𝗿 𝗳𝗲𝗮𝘁𝘂𝗿𝗲𝗱 𝘁𝗮𝗹𝗸! Join Rocco Zerlotti for Session 1: Exploring Electrogenicity: Electrophysiological Approaches to Investigate Membrane Transporters Presentation: "Solid supported membrane-based electrophysiology reveals multiple electrogenic events associated with GABA, Na+, and Cl- binding to human GAT1" Date: October 30, 2024 Time: 9:10 AM EDT Why attend? If you're involved in membrane transporter research, this session will provide groundbreaking insights and practical applications that could revolutionize your studies. We look forward to meeting you and discussing how Nanion can support your research with high-throughput, flexible solutions! For more details about the meeting, visit: https://ow.ly/L7ae50TPjBV #Conference #MembraneTransporters #SSMElectrophysiology #MembraneBiophysics #ElectrogenicTransport

Good morning, everyone! We're kicking off the second day of the 𝗡𝗮𝗻𝗶𝗼𝗻 𝗨𝘀𝗲𝗿 𝗠𝗲𝗲𝘁𝗶𝗻𝗴, and we’re looking forward to another day full of science. We’re getting ready for the first talk of the day from Stefan Kubick (B4 PharmaTech), who will share insights on cell-free systems for the production and functional characterization of membrane proteins. It’s sure to be a great start to the day! Let’s gear up for more exciting presentations, discussions, and learning. Looking forward to spending another day with all of you! #NanionUserMeeting #Electrophysiology #APC #MembraneBiophysics #CellAnalytics

The first day of our Nanion User Meeting is coming to a close. It’s been a full day of interesting talks and discussions, starting with Niels Fertig's welcome, followed by insightful presentations from Martin Gunthorpe, Ulrich Hammes, Prof. Dr. Dr. Christian Grimm, Alexandre SANTINHO, Ph.D. , Adriana Bizior, Surabhi Rajendra Kokane, and Joachim Wegener. We’ve heard today about the potential of KV3 channel modulators for treating epilepsy, impedance-based GPCR assays, and how the intracellular TPC2 channel influences melanoma progression. We’ve also explored the future of organellar target screening, innovative techniques for transporter drug discovery, and new insights into Na+/H+ exchange and auxin transport mechanisms. In between all that, we’ve had poster sessions, flash talks, and instrument demos. Now, we’re heading to dinner at Fräulein Wagner to wrap up the day together. Looking forward to more conversations there! Tomorrow, we’ll continue with another great day of presentations and discussions. Thanks to all the speakers and participants for today – see you in the morning! #NanionUserMeeting #Electrophysiology #CellAnalytics #Networking

We're happy to welcome everyone to our user meeting today! Niels Fertig just gave the opening talk, and we’ve got a lot of interesting things lined up. Today is packed with great presentations, poster sessions, instrument demos, and plenty of time to chat with colleagues. Looking forward to a productive and enjoyable day with everyone! #NanionUserMeeting #Electrophysiology #APC #MembraneBiophysics #CellAnalytics