Carl Baillargeon’s Post

Aspirin could help reduce colorectal cancer in high-risk individuals. Taking aspirin regularly could help lower the risk of colorectal cancer among individuals with unhealthy lifestyles, according to a study by researchers at Harvard T.H. Chan School of Public Health and collaborating institutions. Read: https://lnkd.in/gUpTjtP4 #sciencesdelavie #biotechnology #biotechnology #innovation #innovations #research #sciences #medicaments #research #drugresearch #laboratories #labs #chemistry #chemistry #biology #biologie #biopharma #rna #rnatherapeutics #arn #revolution #happy

!New Breakthrough Publication on ER-Phagy from the carmine Settembre Lab! We are thrilled to announce a groundbreaking study from the Carmine Settembre lab, now featured on the cover of this issue of Developmental Cell. The paper delves into how ER-phagy, a critical cellular cleanup process, is activated by the accumulation of misfolded proteins in the endoplasmic reticulum (ER). Lysosomal storage diseases are a group of disorders characterized by enzyme deficiencies that impair the lysosomal degradation pathway, leading to the accumulation of undegraded substrates in the cell. Targeting pathways like ER-phagy can be crucial for developing therapies for these conditions. In this study, Chiara De Leonibus, Marianna Maddaluno, and their colleagues demonstrate that the ER-phagy response depends on the transcriptional induction of SESTRIN2 and FAM134B, which offers a new pharmacological target to enhance the degradation of misfolded proteins in ER storage disorders. This study not only identifies two potential therapeutic targets for lysosomal storage diseases (LSDs), such as Alpha-1 antitrypsin deficiency and osteogenesis imperfecta, suggesting a unified approach to treating diverse rare conditions. It also importantly underscores the significance of exploring the molecular mechanisms underlying rare genetic diseases to pinpoint common pharmacological targets that could benefit a range of different disorders. The accompanying image vividly illustrates this mechanism, showing "Auto-Pack" (Autophagosome-Packman, in yellow) actively engulfing misfolded proteins (in red) from the complex environment of the ER. #ERPhagy #ProteinMisfolding #CellBiology #LysosomalStorageDiseases #Biochemistry #ScientificResearch #DevelopmentalCell #AcademicPublishing

RNA Repeat Targeting as Potential New Therapeutic Strategy for ALS and Frontotemporal Dementia. Researchers in the US report a potentially new therapeutic strategy for amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Using an optimised Cas13d-based RNA targeting system, they demonstrate significant in vitro and in vivo reduction of the RNA repeat mutations most commonly identified in patients with ALS or FTD. Read: https://lnkd.in/e6pSy2Hx #sciencesdelavie #biotechnology #biotechnology #innovation #innovations #research #sciences #medicaments #research #drugresearch #laboratories #labs #chemistry #chemistry #biology #biologie #biopharma #rna #rnatherapeutics #arn #revolution #happy

Find below the very first article from the Storelli Lab! Exciting research from Max about the nuclear receptor Hnf4 and lipid metabolism regulation. Don't miss it!

Thank you Dr. Rahul Joshi for guiding me on the various fascets of biotechnology, showing me the state of art laboratories at HiMedia Laboratories Pvt. Ltd. and explaining to me how the various functions work. It was a valuable experience and I learned a lot more during our interaction than what is mentioned above. Looking forward to more such enriching interactions. MIT-WPU Department of Biosciences & Technology #biotechnology #science #biology #microbiology #stemcells #technology #biosciences #molecularbiology #immunology #virology

Investigate intracellular bacterial pathogen mediated manipulation of host cellular functions- through live-cell imaging! https://lnkd.in/gDgawDxN ✨ This method by Madalina Mocanita, Kailey Martz, Vanessa D'Costa at University of Ottawa provides a quantitative assessment of how Salmonella manipulates host epithelial cell trafficking to lysosomes using a fluorescent marker, DQ-Red BSA. ✨This live-cell imaging assay can be adapted to study various pathogens and host cell types, offering insights into pathogenesis mechanisms and potential therapeutic targets. Click on the link 🔗 to learn more ! This work has been reviewed by Jinglin Lucy Xie and Xin Xu. #Pathogenesis #bacterial #LysosomalPathway #microbiology Do you have a protocol that can contribute to scientific clarity and reproducibility ? Visit us at https://lnkd.in/dt_igxRi and explore publishing with Bio-protocol at https://lnkd.in/gR3QGYz6! #openaccess #reproducibility #lifesciences #research

It's so exciting to see this paper in print! Here's the correct link to the paper:https://lnkd.in/gwCiWkpj

Congratulations to Prof. Scarlet Shell on the publication of her research in the Antimicrobial Agents and Chemotherapy Journal of the American Society for Microbiology! Titled "Loss of glycerol catabolism confers carbon-source-dependent artemisinin resistance in Mycobacterium tuberculosis" the paper features contributions from current Biology & Biotechnology postdoc Hilario Cafiero, current Bioinformatics & Computational Biology PhD student Junpei Xiao, and two former Shell lab postdocs, Carla Martini and Natalia Alonso. Abstract: In view of the urgent need for new antibiotics to treat human infections caused by multidrug-resistant pathogens, drug repurposing is gaining strength due to the relatively low research costs and shorter clinical trials. Such is the case of artemisinin, an antimalarial drug that has recently been shown to display activity against Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis. To gain insight into how Mtb is affected by artemisinin, we used RNAseq to assess the impact of artemisinin on gene expression profiles, revealing the induction of several efflux pumps and the KstR2 regulon. To anticipate the artemisinin resistance-conferring mutations that could arise in clinical Mtb strains, we performed an in vitro evolution experiment in the presence of lethal concentrations of artemisinin. We obtained artemisinin-resistant isolates displaying different growth kinetics and drug phenotypes, suggesting that resistance evolved through different pathways. Whole-genome sequencing of nine isolates revealed alterations in the glpK and glpQ1 genes, both involved in glycerol metabolism, in seven and one strains, respectively. We then constructed a glpK mutant and found that loss of glpK increases artemisinin resistance only when glycerol is present as a major carbon source. Our results suggest that mutations in glycerol catabolism genes could be selected during the evolution of resistance to artemisinin when glycerol is available as a carbon source. These results add to recent findings of mutations and phase variants that reduce drug efficacy in carbon-source-dependent ways. https://lnkd.in/gwCiWkpj #wpi #wpibiologybiotechnology #biologyandbiotechnology #microbiology #ASM #americansocietyofmicrobiology #biologyresearch #AntimicrobialAgentsandChemotherapy #journalpublication #postdoc #shelllab

HELLO EVERYONE! In our latest video on Bioinsights Academy, where we dive into the 2024 Nobel Prize in Physiology and Medicine, awarded to Victor Ambros and Gary Ruvkun for their groundbreaking discovery of microRNAs! 🏆🔬 MicroRNAs have revolutionized our understanding of gene regulation, and their discovery has had a profound impact on fields like cancer research, developmental biology, and more. In this video, I walk you through the fascinating journey of how Ambros and Ruvkun uncovered the role of these small yet powerful molecules. If you're curious about how microRNAs work, their biogenesis, and their role in human health and disease, check it out here: 👇 🎥 Thank you for watching, and don’t forget to like, comment, and share! Let’s continue learning and exploring the wonders of science together. 🌱🔍 #NobelPrize2024 #microRNA #GeneRegulation #Biotechnology #BioinsightsAcademy #ScienceEducation #VictorAmbros #GaryRuvkun #MolecularBiology #LifelongLearning #LinkedInLearning https://lnkd.in/d56cWSU9

Hi all, I hope you ‘re enjoying the new year. For those of you who are new to Fennik Life Sciences, we offer 3D cell culture products and services for biomedical research and pre-clinical drug testing. Here, on this page, we talk mainly about peer reviewed articles on various topics related to cell and molecular biology research, including mechanisms of chronic diseases.  Of course, we are always interested in 3D cell culture models. We do our best to make sure the articles are accessible to everyone through PubMed. Ok, let’s get started.   Metabolic reprogramming is a hallmark of chronic diseases such as cancer. In cell culture, changes in metabolism are reflected by changes in the rate of extracellular acidification (ECAR) due in part to lactate and increased oxygen consumption (OCR). These phenotypes may be measured over time using the Seahorse XF Analyzer. While it is common to perform Seahorse assays in monolayer cultures, the following article reveals a way to do this in a 3D culture format, specifically in organoid cultures. This is a useful article that includes helpful figures, detailed lists of reagents with catalogue numbers and step by step approaches on culturing organoids (intestinal) and how to measure ECAR and OCR using the Seahorse XF Analyzer. It may be possible to adapt these approaches to different 3D cell culture models. For more information, you can access this article on PubMed: https://lnkd.in/gYtYvXMP   #FennikLifeSciences #FennikLifeSciencesDiscussions #organoidcultures #metabolism #SeahorseXF #extracllularfluxanalysis