Dr. Ayo Olufade, Ph.D.’s Post

Prof. Cech An excellent summary and perspective. From the article, "we are still only beginning to unlock its (RNA) potential." FYI - while scientists continue to unlock the mystery of RNA, we continue to file patent applications. FYI - regarding the 'dark matter' you mention, it's worth noting that this refers to endogenous ancestral nucleotide sequences, a key area of our commercial development. Thank you, Professor Cech, for your enormous contributions to RNA biology. I welcome you to the Golden Age of Virology. https://lnkd.in/e2Gs6j2W Howard

🚀 Exciting News! 🚀 We’re thrilled to announce our latest publication in Algorithms for Molecular Biology! 🎉 Our paper, "A new consensus tree metric based on pairwise distance distributions," explores novel approaches to consensus methods with potential applications in bioinformatics and phylogenetics. 🌿🔬 Discover how these methods can enhance lineage tree analysis, especially in immune cell research, such as B cells. Check it out on ResearchGate: 🔗 Read the full paper: https://lnkd.in/eA2UvaKB #Bioinformatics #ConsensusTrees #LineageTrees #Bcells #ComputationalBiology

Molecular Cell Biology (9th International Edition) - PDF Download for only $22.00

"𝗠𝗼𝗹𝗲𝗰𝘂𝗹𝗮𝗿 𝗯𝗶𝗼𝗹𝗼𝗴𝘆 𝗶𝘀 𝗷𝘂𝘀𝘁 𝗮𝗯𝗼𝘂𝘁 𝗴𝗲𝗻𝗲𝘁𝗶𝗰𝘀 𝗮𝗻𝗱 𝗗𝗡𝗔". This long-standing myth is far from the truth. Let's debunk: 👉 𝗠𝗼𝗿𝗲 𝘁𝗵𝗮𝗻 𝗷𝘂𝘀𝘁 𝗗𝗡𝗔: While DNA is an important part, molecular biology actually extensively studies RNA, proteins, and biochemistry to understand the fundamental processes of life. 👉 𝗧𝗲𝗰𝗵𝗻𝗶𝗾𝘂𝗲𝘀 & 𝘁𝗼𝗼𝗹𝘀: It's not just about "reading" genetic codes. It is also the study and application of crucial techniques like Polymerase Chain Reaction (PCR), gel electrophoresis, etc. 👉 𝗜𝗻𝘁𝗲𝗿𝗱𝗶𝘀𝗰𝗶𝗽𝗹𝗶𝗻𝗮𝗿𝘆 𝗻𝗮𝘁𝘂𝗿𝗲: Molecular biology doesn't exist in a vacuum. It intertwines with other scientific areas like bioinformatics, biophysics, and even medical diagnostics. Molecular biology, contrary to the myth, is not merely "DNA science". It is a broad, multifaceted discipline that pulls together different areas of life sciences. The importance of understanding and acknowledging this diverse field of study is more relevant now than ever. Your thoughts #MolecularBiology #Genetics #DNA #RNA #Proteins #Biochemistry #PCR #GelElectrophoresis #Bioinformatics #Biophysics #MedicalDiagnostics

🔬 **Revisiting Telomere Biology: Unveiling the Dual End-Replication Problem** 🔬 Half a century ago, the discovery of telomeres by scientists Jim Watson and Alexey Olovnikov shed light on a fundamental puzzle: how our DNA gets copied accurately during replication. The subsequent identification of telomerase by Liz Blackburn and Carol Greider seemed to solve the mystery. However, new research published in Nature challenges this conventional wisdom, revealing a deeper complexity. Recent findings suggest not one, but two end-replication problems, intricately tied to the replication of both strands of DNA. While telomerase was known to address the leading-strand problem, it turns out another molecular complex, CST-Polα-primase, tackles the lagging-strand issue. The leading-strand problem arises because the DNA replication machinery fails to fully duplicate the telomere, leaving it with a blunt leading end. Telomerase adds repeats to solve this issue. However, Hiro Takai's discovery revealed a surprising lagging-strand problem: the replisome cannot fully synthesize the lagging strand, leading to further telomere shortening. Joseph T. P. Yeeles' in vitro experiments confirmed this, showing that the replisome stops lagging-strand synthesis before reaching the 5' end. This revelation challenges previous models of telomere replication and necessitates a reevaluation of our understanding of telomere biology. Takai's subsequent in vivo assays further confirmed these findings, shedding light on how CST-Polα-primase replenishes repeats to maintain telomere integrity. This newfound understanding not only revises textbooks but also holds clinical significance. Mutations in CST-Polα-primase are associated with telomere disorders like Coats plus syndrome. By unraveling the intricacies of telomere maintenance, this research opens doors for potential therapeutic interventions in addressing these devastating disorders. In essence, this study marks a significant step forward in our comprehension of telomere biology, highlighting the complexity of DNA replication and its implications for human health. #TelomereBiology #DNAReplication #MedicalResearch 🧬✨

Corina Blanas serves as the Manager of Biomedical Science for UGenome. In this role, she bridges product development and bioinformatics services informed by her experience with NGS, molecular biology, and genomics She is finishing her M.S. in Biomedical Science at Arizona State University. One of her goals is to combine homeopathic medicine with bioinformatics. In her free time, she likes to bake and tinker. #ugenome, #ugenomeai, #pharmacogenomics, #pharmacogenetics, #bioinformatics, #genetics, #dna, #research, #genomics, #medicine, #drugdiscovery, #datascience, #biostatistics, #pharma, #genomicmedicine, #sequencing, #dataanalysis, #precisionmedicine

Molecular biology is a fundamental discipline within the life sciences that investigates the molecular underpinnings of biological activity. It bridges the gap between genetics and biochemistry, providing a deep understanding of the interactions between various biomolecules within cells. This field is crucial for elucidating how genetic information is encoded, replicated, and translated into functional proteins, and how these processes are regulated. The Core Concepts of Molecular Biology At the heart of molecular biology are nucleic acids—DNA and RNA. DNA (deoxyribonucleic acid) carries the genetic blueprint of an organism, while RNA (ribonucleic acid) acts as a messenger and intermediary in translating this genetic information into proteins. Molecular biology seeks to understand the mechanisms of DNA replication, transcription (the process of copying DNA into RNA), and translation (the synthesis of proteins based on RNA sequences).

#RNA helps to unlock #life’s deepest #secret Step aside #DNA. RNA has arrived Opinion NYT Thomas #Cech, #Nobelprize winner Some 75% of the human #genome consists of dark matter that is copied into RNA’s of unknown function. We are still only beginning to unlock it’s potential. #ribonucleicacid #mRNA #biology #biochemistry #research https://lnkd.in/denVKu4Y

Eva Nogales and Julia Mahamid have just released a new Review Article in Nature Portfolio called, "Bridging Structural and Cell Biology with Cryo-Electron Microscopy". Cryo-EM and Cryo-ET are techniques we use to perform 3D visualization #biomacromolecules in #drugdevelopment and produce characterization. CATUG has partners with programs entering Clinical Studies in markets all over the globe, and the #regulatory requirements are Regulatory Authority (RA) dependent. For instance, the NMPA-National Medical Products Administration calls for more extensive biophysical characterization than other regions; one such distinction is when it comes to performing cryo-EM for #LNP development. No matter the region, the characterization benefits and implementation of #cryoem and #cryoet for your RNA-LNP drug products should be considered. https://lnkd.in/eVEkU9fR https://lnkd.in/emc8qfgr #cellbiology #molecularbiology #biology #research #researchtools #celltherapies #genetherapies #geneediting #cgt #science University of California, Berkeley EMBL

Day 4 of reviewing cool papers I come across. Title: Cell-free synthesis of infective phages from in vitro assembled phage genomes for efficient phage engineering and production of large phage libraries Synthetic biology. Aug 24, 2024. Kristensen et al. This study doesn't unveil new phages or viral features, but presents a powerful new toolkit for phage engineering. By combining optimized Golden Gate assembly with cell-free TXTL reactions, they achieve unprecedented efficiency (just in 1 day vs a week!) in building and rebooting phage genomes. This new method enables the generation of large, diverse phage libraries, paving the way for high-throughput screening and personalized phage therapies. This is how it was done in the study: (1) Optimizing Golden Gate Assembly for phage genomes: they divided the T7 phage genome into 9 fragments for assembly. (2) Doing cell-free TXTL reactions using the commercially available myTXTL Linear DNA Expression Kits (Arbor Biosciences), without the need for a host cell (3) Creating a barcoded phage DNA library by incorporating random DNA barcodes during assembly (4) Sequencing to analyze library diversity to confirm the creation of a large library with millions of unique phages Article link: https://lnkd.in/d8B9qzfi !