Elad Bar Natan’s Post

-Primer Design “Manually”- Primer design is one of the skills that is needed to conduct molecular biology or genetics. There are many websites or software that have been used. The utilisation of each platform makes the tasks easier. Users input the nucleotide sequence and the platforms will work automatically to find the candidate of primers. However, in this time I’ll share my experience when designing primers without using these automatic platforms. Here are the steps of primer design “manually”. 1.   Data retrieval. Because my reference sample has been sequenced wholly, I can get the data of the gene at the level of gDNA and mRNA. In my case, my sample is Phalaenopsis amabilis and my reference sample is Phalaenopsis equestris. 2.   Gene mapping. The data is aligned to confirm the region of exon and intron. The software I use is Unipro UGENE. 3.   Sequence selection. I choose 18-25 nucleotides from each part of CDS gene region that can be a candidate for oligonucleotides. 4.   Ensuring Candidate Oligonucleotides. The selected nucleotides are analyzed on OligoAnalyzer Tool website, a web from IDT, to check their melting temperature, hairpin structure, self-dimer, etc. Nucleotides that match the primer criteria can be used for PCR reaction. Based on my experience, the designed primers may have high specificity to the region, because users design it from their reference directly. In contrast, the specificity will be lower if the primers amplify other species. #molecularbiology #primerdesign #researchexperience #sharing

Are you curious about the latest advancements in biotech instrumentation? Check out Pala, a cell sorting instrument developed by Bio-Techne, designed to provide high-speed and gentle sorting of cells from diverse samples with superior purity. With Pala, researchers can easily sort cells for downstream applications such as single-cell analysis and gene expression profiling, leading to more accurate results. If you're looking for a reliable and efficient cell sorting solution, Pala is definitely worth exploring! Explore the Pala™ Platform: https://bit.ly/3Xp6Gw8 If you are interested in learning more about the applications, contact me for a meeting and we will have the presence of our specialist Romain Useo to better understand the applications and benefits of using Pala instrument #CellSorting #PalaByBiotechne 🦾💻🔬

Cell-free systems provide a powerful tool to engineer novel systems without the constraints of living cells. These systems consist of the cellular machinery necessary for gene expression and protein synthesis, but without the presence of living cells. Due to their inherent safety, simplicity and portability, cell-free systems have become an increasingly important tool in iGEM and synthetic biology more broadly... READ MORE: https://lnkd.in/ePxk5ndM Yorgo El Moubayed - يورغو المبيّض #cellfree #synbio #syntheticbiology

Hello Connections ! I had the opportunity to Complete a Certificate Course " Advanced Bioinformatics Certification course" Under the Guidance of Dr. Nikita Bora Ma'am Whose teaching style is amazing. Through this course I learnt About. 1) Gene , Genome & Protein Retrieval Through NCBI 2) Sequences Alignment, Blast , clustal omega 3) Gene prediction in : 1. Prokaryotic, 2. Vertebrate 3. Eukaryotic's 4) Identification of pathogenic islands 5) Prediction of Antibiotics resistance gene (AMR) , CARD 6) Protein Structure Analysis i.e Swiss Model , Alpha Fold2 ,Raptorx 7) STRIDE , IUPred , PDBsum 8) Generation of Mutant structures 9) Phylogeny 10) Computer- Aided Drug Discovery - CADD -Molecular Docking - CAST-P -Swiss Design -Lipsinki's Rule -SwissDock #BioGrademy #Bioinformatics #AdvancedBioinformatics #Certification #Research #Biotechnology #Course #Biotechnology #Bioinformatics BioGrademy

|The third day was the perfect finale|💫 😎 On third Day of Work Shop on Basic Molecular Biology Techniques|💫 ---------------------------------------!!!!!! We dove into agarose gel electrophoresis, experiencing this critical technique for DNA analysis. Additionally, we explored In-silico gene sequence analysis!!!🧪🧬 -----------------------------------------!!!!!!! ||"Testing DNA samples to analyze fragment sizes & & & Visualizing DNA bands through Gel Electrophoresis Then Capturing the magic of DNA migration in the Gel :|| 😍😀🧬 #MolecularBiology #Biotechnology #PCR #DNAIsolation #GelElectrophoresis #LearningJourney #BiotechStudent #HandsOnExperience

Unlock lab efficiency with the Thermo Series—Precise heating, cooling, and mixing. • Provides a streamlined solution for complex laboratory processes.  • Saves time and reduces error, enhancing overall lab productivity.  • Ideal for researchers looking for reliable and versatile lab equipment. Your solution for precise heating, cooling, and mixing.  Ideal for Gene Synthesis, Gene Purification, Protein Denaturation, Enzymatic Reactions, and Bacterial Growth. Its precision and efficiency make it an indispensable tool for research in Molecular Biology, Biochemistry, and Genetics. Experience unparalleled control and versatility in your lab work. Whatsapp : https://lnkd.in/d8k4u2WZ Eamil : sales@caresourceglobal.com Website : https://lnkd.in/dZa6sHrF #Thermoseries #PreciseHeating #Cooling #Mixing #LaboratoryEquipment #TemperatureControl #ScientificInstruments #ChemistryLab #PharmaceuticalLab #ResearchTools #HeatSensitiveMaterials #LaboratoryAutomation#ChemicalEngineering #ExperimentalSetup #DigitalControl #ThermalManagement #TemperatureAccuracy #ThermalCycling #MixingTechniques #LaboratoryInnovation

🧬Avidity sequencing🧬 🧬🔍A new method for DNA sequencing that optimizes how we read DNA. By using specialized chemistry, we can identify nucleotides more efficiently. This approach uses unique nucleotide compounds to create strong bonds with DNA copies, lowering the amount of chemicals needed and improving accuracy. Avidity sequencing achieves impressive accuracy rates, with minimal errors even during challenging sequences. This breakthrough method promises more reliable and precise genetic analysis. 🔍🧬 #DNASequencing #Genomics #Biotechnology #Innovation #Sequencing #LifeSciences Ref: https://lnkd.in/g-XWV2q8

A comprehensive study comparing different chromatin loop callers. Our tools FitHiChIP (for HiChIP) and FitHiC2 stand out from the rest considering various performance metrics.

On this day in Science Jennifer Doudna was born. Doudna and Charpentier received the 2020 noble prize in chemistry for their work on CRISPR/Cas9 CRISPR/Cas9 edits genes by precisely cutting DNA and then letting natural DNA repair processes to take over. The system consists of two parts: the Cas9 enzyme and a guide RNA. Rapidly translating a revolutionary technology into transformative therapies. #science #chemistry #stem #genetics #crisprcas9 #womeninscience #geneediting #womeninscienceday #Science #sciencecommunication #biochemistry #genetherapy #technology #womenandgirlsinscience #cas9 #biopharmaceuticals #sciencewillwin #infographic via Compound Interest | Chemistry infographics

Molecular biology and recombinant DNA technology are foundational to biotechnology. Molecular biology helps us understand the structure and function of genes, proteins, and other cellular components, enabling precise manipulation of biological systems. Recombinant DNA technology allows scientists to combine DNA from different sources, creating genetically modified organisms (GMOs). These technologies are crucial for advancing research, solving global challenges, and fueling biotechnology's growth in various sectors. To know more, go through the blog about "Introduction to Biotechnology". Link is below in the comment section. #biotechnology #biology #DNA #RNA #protein #growth #genes #technology #research #science #scientists #structure #cell