JMSACL (Journal of Mass Spectrometry & Advances in the Clinical Lab)’s Post

Discover how the latest advances in high-resolution mass spectrometry (HRMS) are transforming Drug Metabolism and Pharmacokinetics (DMPK). Our new poster highlights the shift from traditional triple quadrupole systems to cutting-edge HRMS instruments, like the Thermo Scientific™ Orbitrap Exploris™ 120, for more informative data acquisition. Find out how we: ➡ Achieve reliable sensitivity and excellent dynamic range in full scan analysis with high resolution and accurate mass capability. ➡ Save significant time by eliminating the need for MS/MS method development. ➡ Handle challenging compounds, including PROTACs and peptides, with ease. ➡ Identify metabolites and potential issues in a single analysis. Learn how the Orbitrap Exploris™ 120, paired with the Thermo Scientific™ Vanquish UHPLC system, delivers robust, sensitive, and reproducible data for both in vitro and in vivo DMPK assays. 📄 Read the Full Poster Here: https://lnkd.in/eGniZv9E #metid #drugdiscovery #adme #dmpk #metabolites

Source: Analytical chemistry This article presents a sensitive and comprehensive method for analyzing signaling lipids using targeted liquid chromatography-mass spectrometry (LC-MS/MS). The method overcomes challenges such as a wide dynamic range, isomeric and isobaric species, and unwanted interaction along the separation path. It allows for the monitoring of 388 lipids in a single run of only 20 minutes and has been successfully applied to analyze the human plasma signaling lipidome and investigate lipid signaling pathways during platelet activation. This method provides unprecedentedly comprehensive analysis and direct insight into signaling pathways.

We are excited to announce the recent publication of our customers' study in Journal of Chromatography A, titled "In-depth Characterization of a Cysteine-Linked ADC Disitamab Vedotin by Multiple LC-MS Analysis Methods and Cutting-Edge Imaged Capillary Isoelectric Focusing Coupled with Native Mass Spectrometry." This research presents a state-of-the-art iCIEF-MS technique under native conditions for detailed analysis of the cysteine-linked ADC, Disitamab Vedotin, in conjunction with traditional online LC-MS approaches. The iCIEF-native MS method preserves the native state of the ADC during both iCIEF separation and MS detection, while also enabling straightforward method transfer from development to quality control (QC). If you're interested in iCIEF-native MS for protein heterogeneity, please refer to the following link: https://lnkd.in/gBUvRWRp

In a recent publication, Merck researchers discovered the importance of incorporating flow into preclinical in vitro models. When comparing intestinal permeability of oral peptides, conventional 2D static cultures displayed a 4000-fold increase in permeability, whereas the microfluidic Gut-on-a-Chip model displayed a 20-fold increase, which is in line with ex vivo and in vivo preclinical models: https://hubs.ly/Q02H5VH90

Scientific publications require creation of hypotheses, experimental and assay development, careful analysis of results to ensure reproducibility, and trusted reagents. Our catalog of buffers are tested and trusted. See the number of publications that cite our products in the chart below and check out some recent peer reviewed publications cited with our products: https://lnkd.in/eZg5fXtR #custombuffers #customreagents #lifesciencebuffers #lifesciencereagents #diagnostics #biosimilar #bioprocessing #assaydevelopment 

Quantification of virus like particles (VLPs) is challenging due to the large size and structural heterogeneity of the molecule. Structural heterogeneity arises due to random assemblage of L1 protein with other host cell proteins. In this study, we propose use of Reversed phase chromatography for separation and identification of VLP L1 proteins in inhouse crude and purified samples and quantification of L1 protein at different process points. The proposed method takes about 20 min and has been demonstrated to be highly sensitive, robust, and selective as confirmed through mass spectrometry. #hplc #biotherapeutics Free to download: https://lnkd.in/dJumkgcd

We are delighted to announce that on April 5, 2024, the results of an observational study comparing a brand-new point-of-care fluorescence immunoassay for determining circulating levels of vitamin D with ultra-high performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) were published in the prestigious peer-reviewed journal "Molecules." Vitamin D deficiency and insufficiency, which can lead to many pathological conditions, have become a widespread health problem, resulting in an increase in requests for determining circulating levels of 25-hydroxyvitamin D (25-OH-D), that is currently the main indicator of vitamin D status and it is performed by different assays. However, the analytical performance of these immunoassays is highly variable and does not necessarily provide comparable results. To address this issue, considerable efforts have been made to improve the metrological performance of this test to achieve comparable results across different methods and manufacturers. In particular, the AFIAS Vit D assay, a point-of-care fluorescence immunoassay based on a single cartridge with a lateral flow architecture and pre-filled with all the necessary reagents for the test, showed a good correlation with LC-MS/MS when running on both the Afias-1 and Afias-10 analyzers (Boditech and Menarini). Therefore, we can confidently assert that AFIAS-1 and AFIAS-10 are reliable instruments for accurately and rapidly measuring 25-OH-D levels. #innovation #healthcare #technology Full Pdf link: https://lnkd.in/dSHVAyXf

Some nice work by Coombes et al, looking at ssDNA assay for product purity 🛎Overview: Coombes et al have developed a novel method using ion pair reversed phase HPLC assay for ssDNA re-generation post PCR for selective enhancement of ligands by exponential enrichment (SELEX). But SELEX experiments often result in low yields of ssDNA aptamers and the ability to generate high-quality ssDNA in high yields from the dsDNA PCR products. 🎯 Summary: Coombes et al IP RP HPLC assay demonstrating significant advantages over existing affinity chromatography and nuclease digestion methods, for the purification of ssDNA . #massspectrometry #massspec #nucleotides #dna #lcms #hplc #chemistry #science #sciencecommunication #scienceandtechnology

Source: Biomedical chromatography : BMC A liquid chromatography-tandem mass spectrometry method with vonoprazan fumarate-d4 as a stable isotope-labeled internal standard was developed and validated for the quantification of vonoprazan fumarate in human plasma. The method involved protein precipitation and gradient elution using formic acid and acetonitrile. The linear range of quantification was 0.150-60.000 ng/ml. The method was fully validated and successfully applied in a bioequivalence study of vonoprazan fumarate tablet among healthy Chinese volunteers.

Source: Bioanalysis A new LC-MS/MS method was developed to determine Lifitegrast in human plasma and tear for pharmacokinetic studies. The method was validated and successfully applied to clinical samples. This method is useful for evaluating Lifitegrast in plasma and tear.