Justin Vento, Ph.D.’s Post

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Visiting Assistant Teaching Professor at Villanova University

New technology alert! Introducing IMPRINT, a cell-free method for rapidly methylating DNA to increase its transformation into bacteria. https://lnkd.in/dNFpDxnj The vast world of bacteria holds great potential to develop novel products in diverse fields, from sustainable chemical factories to living therapeutics that combat disease and promote well-being in humans as well as in agricultural crops. However, studying and engineering bacteria is largely hindered by an inability to deliver DNA into their cells, often due to the presence of bacterial defense systems that eliminate foreign DNA. As a result, current efforts mostly rely on the few "model" bacteria such as E. coli that can be engineered -- but what if we can tap into the multitude of other bacteria that possess unique and often superior traits specific to their environment? Using IMPRINT, we show increased DNA transformation in relevant pathogenic (Salmonella) and probiotic (Bifidobacteria) strains of bacteria, develop a method to quickly decipher the "optimal" methylation pattern on transformed DNA for a particular strain, and illustrate how the boost in transformation facilitates the use of high-throughput screens in "non-model" bacteria. It's been super fun and rewarding to lead this project and see it develop over the years into something useful, and it took a full team effort! Huge shoutout to everyone involved Chase Beisel Nathan Crook Deniz Durmusoglu Tianyu Li John van Schaik Sean Sullivan Fani Ttofali Lars Barquist Costas Patinios Yanying Yu and Yanyan Wang!

Explore scientific, technical, and medical research on ScienceDirect

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Jodi Gerver

Dean at STEMCivics Charter School Educational Leader

3mo

Super awesome 👏

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Christopher Kitchens

Chemical and Biological Engineering Professor

2mo

Very Cool. Congrats Justin!

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Ryan Leenay, PhD

Associate Vice President, Genome Editing

3mo

Congrats Justin!!

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