Marine Ledru’s Post

Join us and special guest speaker Prof. Dr. Nicole Endlich, Dept. of Anatomy and Cell Biology, Universitätsmedizin Greifswald and CEO of NIPOKA GmbH, Germany, for this LIVE webinar. To register ➡️ https://lnkd.in/dHWPX2-Z Prof. Nicole Endlich will give an overview of her research on kidney disease and the development of new diagnostic tools. She will speak on: ○ Developing high-content screening methods based on a zebrafish larvae model for the identification of potential therapeutic agents ○ Combing super resolution-based imaging techniques with multiplex staining and in situ hybridization for the advanced visualization of structure and morphology #diagnostics #drugdevelopment #microscopy

🔬 Exciting Insights from TIGEM Research! 🔬 This week, we delve into the groundbreaking research led by carmine Settembre at TIGEM. Carmine is an outstanding researcher and Full Professor in Histology and Embryology at the University of Naples “Federico II”. His remarkable achievements include the prestigious Ian Boyle award from the European Calcified Tissue Society (ECTS) and being elected as an EMBO Young Investigator. Carmine has secured grants like ERC StG, ERC PoC, ERC Consolidator from the European community for his cutting-edge work on autophagy roles in health and diseases. Carmine's focus lies in unraveling signaling pathways controlling selective autophagy and lysosome biogenesis in response to physiological stimulations. His ultimate goal is to design tailored therapeutic approaches for diseases marked by intracellular accumulation of toxic materials. Diseases like #mucopolysaccharidosis, #alpha1antitrypsin, and #OsteogenesisImperfecta fall within the purview of Settembre Carmine's studies. 📚 If you're keen to explore the role of lysosomes in orchestrating key cellular functions, don't miss the insightful review recently published in Nature Review Molecular Cell Biology. Carmine co-authored this piece with @Rushika perer Rushika M. Perera, an expert in autophagy-lysosome activation mechanisms at University of California, San Francisco. 🔗 Read the full review here: https://lnkd.in/dN6Xq23Q Stay tuned for more updates on cutting-edge research and scientific advancements! 🌐 #Science #Research #Autophagy #Lysosomes #HealthcareInnovation #Tigem #telethon

Phospholipids in murine retina --- Researchers developed a non-radiolabeling methodology to profile and quantify phosphoinositides and phosphatidylinositol in the mouse retina. Additionally, they identified subcellular localization patterns of these phosphoinositides within photoreceptors in the mouse model. The authors analyzed PI(4)P and PI(4,5)P2 in the retinas of dark- and light-adapted mice and did not reveal any major differences. Lipidomics analysis showed that over 82% of the total phosphatidylinositide pool in the mouse retina comprises arachidonic acid-containing species, PI 38:4, and PI 36:4. The main distinction in distribution is that PI(4)P was present in the light-sensitive outer segment, while PI(4,5)P2 was mostly not present there yet present in photoreceptor synapses. Read the article here: https://lnkd.in/e6UvxZv7 #research #lipid #lipidomics #LipidMetabolism #lipotype #lipidome #retina #EyeLipidomics

Join us and special guest speaker Prof. Dr. Nicole Endlich, Dept. of Anatomy and Cell Biology, Universitätsmedizin Greifswald and CEO of NIPOKA GmbH, Germany, for this LIVE webinar. To register ➡️ https://lnkd.in/dr9R5ww9 Prof. Nicole Endlich will give an overview of her research on kidney disease and the development of new diagnostic tools. She will speak on: ○ Developing high-content screening methods based on a zebrafish larvae model for the identification of potential therapeutic agents ○ Combing super resolution-based imaging techniques with multiplex staining and in situ hybridization for the advanced visualization of structure and morphology #diagnostics #drugdevelopment #microscopy

💡 Discover more when spatial biology meets single cell seq. 💡 🔊 Researchers at Karolinska Institutet in Sweden, Egle Kvedaraite and Mattias Svensson, published an article titled "Intestinal stroma guides monocyte differentiation to macrophages through GM-CSF" in the journal "Nature Communication". The team used single-cell seq, flow cytometry analysis, and #MACSima spatial biology technology, focusing on the stromal cell profiling in pediatric inflammatory bowel disease (IBD) samples and their distribution in various layers of the intestine, as well as their interaction with immune cells. The Spatial biology results on the #MACSima platform show that there is a co-localization relationship between fibroblasts and monocytes/macrophages in the intestinal microenvironment of IBD patients. In vitro experiments further confirmed that stromal cells promote the transformation of monocytes into macrophages by releasing GM-CSF cytokines. This finding may provide useful help for the development of new treatments for IBD in the future ✒  This article is again a great example to show multi-omics approach (single cell combined with spatial biology) can help you discover more   ✒ How spatial biology can provide valuable insights into the complex interaction between cells.   ✒ How spatial biology can provide guidance for potential treatment targets discovery. Link to the publication: https://lnkd.in/ewJQ33_v #MACSima #Spatialbiology #Spatialmultiomics

🎉 Congratulations to Lion Raaz, his supervisor Prof. Dr. Petra Knaus, and their team from Freie Universität Berlin and the Max Planck Institute for Molecular Genetics (MPI-MG) on their recent publication in the Cell Press STAR Protocols journal! 🧬 👨🔬 This innovative study presents a detailed ATAC-seq protocol for fluid shear stress (FSS)-stimulated endothelial cells, providing new insights into atheroprone and atheroprotective mechanisms❤️. The research demonstrates how to culture and harvest endothelial cells from microfluidic channels for low-input sequencing assays, with additional exploration of growth factor stimulation and varying FSS rates. ⭐ We’re thrilled to see the ibidi Pump System play a role in replicating FSS mechano-regimes in vitro, supporting this innovative research in cardiovascular biology. 📰 Read the full article here: https://lnkd.in/edjBEPeW Paul-Lennard Mendez Jerome Jatzlau Mundlos Stefan #fluidshearstress #endothelialcells #microfluidics #atherosclerosis #innovation #protocol #research #ibidi #cellpress

Honored to Attend and Speak at MICROPHYSIO2024 in Cargèse Corsica !! It is a true honor to be invited to speak at this incredible conference, where I will be discussing the latest developments in organoid technology and our STEADY STEM technology. As research in the field of organoids and micro-physiological systems progresses, it’s becoming increasingly clear that traditional in vivo models, such as genetically modified mouse models and orthotopic patient-derived xenografts, cannot fully capture the complexity of human physiology. Moreover, in vivo models fall short of complying with the principles of ‘3R’ – Replacement, Reduction, Refinement – reinforcing the need for advanced in vitro alternatives that mimic functional organs and their pathologies. Maybe organoids and organ-on-chip will help in that way… I look forward to exchanging ideas with researchers, bioengineers, and clinicians across multiple fields as we explore how these breakthroughs in micro-physiological systems are shaping the future of healthcare. Once again, many thanks to the Organizing Committee for making this gathering possible. Morgan Delarue, Stéphanie Descroix, Edouard Hannezo, Laurent Malaquin, Danijela Matic Vignjevic,Tomba Caterina. #Organoids #OrgansOnChip #MicrophysiologicalSystems #STEADYSTEM #InVitroModels #3RPrinciples #Bioengineering #HealthcareInnovation #Organogenesis #FutureOfMedicine #Conference #CNRS #INSERM #UCBL #INL #LBTI #CRCL #CLB #HCL #Proanima #FC3R #Lyonbiopole

More exciting news... Our latest application note reveals that Bio-Techne's Pala cell sorter outperforms the traditional method of sucrose gradient preparation in speed, ease of use, and data quality for 10x Genomics single-cell sequencing from brain samples. Special thanks to our own Alberim Kurtishi, Ph.D., our Applications Science Team, and the ExosomeDx Team at Bio-Techne for their pioneering efforts! The below image from our study illustrates how Pala sorting leads to more diverse and evenly distributed cell profiling, retrieving more useful and interesting data across more valid cell clusters. By integrating the Pala, your lab can achieve faster processing times and more precise data, enabling deeper insights into complex brain tissues. Access the full app note here: https://lnkd.in/gMRNjeVa #SingleCellSequencing #Neuroscience #Biotechnology #Innovation #10X #Genomics #BioTechne

Our next speaker, Dragomir Milovanovic is fascinated by how thousands of macromolecules and organelles self-organize at the synaptic buttons, the critical communication sites between neurons. Since January 2020, he has led the Laboratory of Molecular Neuroscience within the German Center for Neurodegenerative Diseases (DZNE). Drago pioneered the concept of liquid-liquid phase separation at the synapse, demonstrating that synaptic vesicles can organize into a distinct liquid phase, akin to oil droplets in water (Milovanovic et al., Science 2018, Hoffmann et al., Nat Comm 2023). The work from his lab showed that the condensates of synaptic vesicles act as a buffer recruiting disordered synaptic proteins such as alpha-synuclein, a protein implicated in the pathology of Parkinson’s Disease. Recently, the lab discovered that condensates can harbor electric potential at their interfaces, suggesting a new function of condensates as mesoscale capacitors that can store charge. Condensate biology is now emerging as a key mechanism for understanding synapse organization (Sansevrino et al., Trends Neursci, 2023) #Horizons2024 #synaptology #phaseseparation

Our latest #bioRxiv paper!!! Totally surprised and amazed that our Patho-DBiT-seq technology can spatially map all #RNA species — mRNA, pre-mRNA, alternative splicing isoform, RNA editing, microRNA, Single Nucleotide Variant (SNV), etc all from a clinical archival #FFPE tissue slide with high coverage at whole genome scale and near single cell resolution. These have been deemed too challenging to study in #FFPE and so glad our Patho-DBIT is making these impossibles possible! So much look forward to its impact on human tissue biology research and potentially clinical histopathology & personalized genome medicine 🤩🤩🤩. Huge team work with Mina Xu , Yi Xing , Jun Lu , Mark Gerstein , Mingyao Li , and many others from Yale, Penn, and CHOP. Yale University , Yale School of Engineering & Applied Science , Yale University School of Medicine , University of Pennsylvania , Penn Medicine, University of Pennsylvania Health System , Children's Hospital of Philadelphia #spatialomics #spatialbiology #pathology #singlecell #rnaseq #microRNA #isoform #splicing #SNV, #mutation, #TMB, #cellbiology #tissueimaging #DBiT-seq, #genomics, #genome, #transcriptomics , #personalizedmedicine #precisionmedicine #cancer #lymphoma #neuroscience #oncology