Matthew Lauber’s Post

You could run this exact experiment on Uncle and directly measure size by DLS, confirm aggregation by SLS, DNA leak by fluorescence with SYBR Gold. And with higher throughput than the other instruments listed. Details here: https://lnkd.in/eb54K2SB

Are you working with mammalian cells? Do you want to know a bit more about the cell density effect? Don't hesitate to have a look at our new publication! Pol Pérez Rubio has studied how the PEI:DNA polyplexes interact with membrane proteins at low and high cell density! Go check it out! #CellDensityEffect #HEK293 #Transfection #ViralParticles #AnimalTechnology #Biotechnology https://lnkd.in/dEZ__s7y

Read and share this article for free, through July 15th. CellShip: An Ambient Temperature Transport and Short-Term Storage Medium for Mammalian Cell Cultures Emma Buick, Andrew Mead, Abeer Alhubaysh, Patricia Bou Assi, Parijat Das, James Dayus, Mark Turner, Lukasz Kowalski, Jenny Murray, Derek Renshaw, and Sebastien Farnaud Cell culture is a critical platform for numerous research and industrial processes. However, methods for transporting cells are largely limited to cryopreservation, which is logistically challenging, requires the use of potentially cytotoxic cryopreservatives, and can result in poor cell recovery. Development of a transport media that can be used at ambient temperatures would alleviate these issues. In this study, we describe a novel transportation medium for mammalian cells. Five commonly used cell lines, (HEK293, CHO, HepG2, K562, and Jurkat) were successfully shipped and stored for a minimum of 72 hours and up to 96 hours at ambient temperature, after which, cells were recovered into standard culture conditions. Viability (%) and cell numbers, were examined, before, following the transport/storage period and following the recovery period. In all experiments, cell numbers returned to pretransport/storage concentration within 24–48 hours recovery. Imaging data indicated that HepG2 cells were fully adherent and had established typical growth morphology following 48 hours recovery, which was not seen in cells recovered from cryopreservation. Following recovery, Jurkat cells that had been subjected to a 96 hours transport/storage period, demonstrated a 1.93-fold increase compared with the starting cell number with >95% cell viability. We conclude that CellShip® may represent a viable method for the transportation of mammalian cells for multiple downstream applications in the Life Sciences research sector. https://lnkd.in/ebY3JpFy #Biobanking #Biospecimen #Biorepository #Biobank #Biopreservation #SampleManagement #ISBER #BiopreservationAndBiobanking

#Article now available! #AAV & #lentiviral vector titer determination: past, present, & future In this article, Mark White (Associate Director, Biopharma and Translational Product Marketing, Digital Biology Group, Bio-Rad Laboratories), John Bechill (Principal Scientist, BioProcess Group, Modalis Therapeutics), and Peng Wang (Senior Scientist, #ViralVector Analytical Team Lead, Lonza) discuss the need for accurate viral titer determination, traditional approaches to measuring viral titer, and the field’s trajectory into the future. Read this article to: • Review the traditional methods used for measuring viral titer for both AAV and LV #vectors • Explore new analytical technology to address the limitations of these traditional methods • Gain insight into how regulators’ opinions and expectations have evolved with regard to methods for accurate viral titer determination • Discover the key emerging analytical methods for AAV and lentiviral vectors Read at your convenience below:

Genomenon and Pharming Collaborate to Advance APDS Diagnosis #AI #APDSdiagnosis #artificialintelligence #Biotechnology #Genomenon #llm #machinelearning #Pharming #PIK3CD #PIK3R1genes #PrecisionMedicine #variantdata

✏ BioProcess International article covering our recent collaboration with FOXG1 Research Foundation 👨👩👧👦 “Working with Rare & Ultra-Rare Disease Foundations, where you are so close to the patient, adds an extra sense of urgency and commitment” #genetherapy #AAV #CDMO #CRL #biotechnology #research #scienceandtechnology

[CALL FOR ABSTRACT IS OPEN📣 ] We are pleased to announce the opening of the Call for Abstract for the 4th Conference on Next Generation Sequencing for Adventitious Virus Detection in Biologics for Humans and Animals. This meeting will be held on December 3 - 5, 2024 in Frankfurt, Germany. This face-to-face meeting will continue to highlight current scientific data and knowledge with focus on NGS implementation for adventitious virus detection in biological products. Discussions will include capabilities of NGS for virus detection in different matrices and validation of the technical and bioinformatics steps involved in NGS for its applications in characterization and safety evaluation of biologics such as vaccines for human and veterinary use, gene and cell therapy products, and biotherapeutics. 📅 Save the date December 3-5, 2024 📅 Deadline for the submission Abstracts are submitted via email at secretariat@iabs.org before 15 May 2024. ℹ Learn more about submission instructions: https://lnkd.in/dMffzQkJ 👉 To see more about the workshop : https://lnkd.in/dvaKFWBa #IABSevent #IABS #meeting #biologicals #virus #callforabstract

After months of ideating and planning with the team at Hitachi High-Tech America, Inc., today marks our official launch of RAMP UP- the Rapid Application to Market Penetration User Partnership program. With these 50 instruments, we will spend the upcoming months driving application development on the #OhmX platform across #raredisease, #cancerresearch, #cellandgenetherapy, #pharmacogenomics and more. 🥂 here’s to one more step in the democratization of structural variant analysis! 🧬 #pressrelease #genomics

Bringing OhmX to the masses! Message me if you would like to learn how this program can help your lab advance your structural variant analysis with high-resolution electronic genome mapping

🧬 The DNA Synthesis Market is set for a remarkable journey! 🌐 Projections suggest it will grow to a whopping USD 20.5 billion by 2032. 🚀 Witness the transformative power of biotech and genomics shaping our future! #DNASynthesis #Biotech #Genomics #FutureTech