NUVISAN The Science CRO’s Post

Berg Luecke et al latest publication supplemental data holds a nugget of gold for those of whom who use PNGase F in their studies.   🎯 Summary:   Here Berg Luecke et al found big differences among vendors which could be problematic for analysis of low input samples. See supplemental S6 image.   “These data provide evidence that enzymes, as supplied by several vendors, contain peptides from PNGase F degradation or cleavage and contaminating proteins. As PNGase F is added to the sample postenrichment and immediately prior to the desalting and concentration step, any contaminants present in the enzyme product will be present in the peptide mixture analyzed by MS. “ “Low purity enzymes will result in fewer surface proteins identified and lower calculated enrichment efficiency of surface proteins even when stringent washing conditions are used to remove nonbiotinylated proteins from the enrichment substrate. Thus, contaminants are important to consider when prioritizing workflow optimizations.”   📌Full Publication available: https://lnkd.in/eGumc6Q2  #massspectrometry #massspec #chemistry #impurties #biopharmaceuticals #biotech #science #scienceandtechnology #sciencecommunication #chromatography #glycan #analyticalchemistry #analytical

Discover the hidden wonders of the microscopic world with our precision microscopes. Perfect for students, professionals, and researchers, advanced technology brings even the tiniest details into sharp focus. Take your research and learning to the next level! For more information visit srdpharma.com #Microscopy #ScienceTools #ResearchEquipment #pharmaceuticals #pharma #chemistry #biology #microbiology #microscopy #research #drugs #Clinical #Laboratory #Scientists #lab #tests

👋 Discover AEBSF with Gojira Fine Chemicals! 🌟 Are you in the life sciences, pharma, or biotech industry? Look no further! 🧪 Gojira Fine Chemicals, an ISO 9001:2015 certified supplier, offers AEBSF, a powerful protease inhibitor. Here’s why it matters: AEBSF (4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride): Function: AEBSF inhibits serine proteases, safeguarding your precious protein samples during extraction and purification. Applications: -Research: Use AEBSF to prevent proteolytic degradation during experiments. -Drug Development: AEBSF plays a crucial role in drug discovery and development. -Biotechnology: AEBSF ensures protein stability in diagnostic assays. -Life Sciences: From cell culture to protein analysis, AEBSF is your ally. 🔗 Learn more about Gojira Fine Chemicals’ offerings at www.gojirafc.com. Remember, our products are intended for research and manufacturing purposes, not for therapeutic use in humans or animals. Connect with us on LinkedIn and elevate your scientific journey! 🚀

🎉 Exciting News! 🎉 I’m elated to share that I’ve recently completed the "Introduction to Liquid Chromatography" course offered by SCIEX. This experience has deepened my understanding of liquid chromatography techniques and their critical applications in various fields, including pharmaceuticals, biotechnology, and environmental analysis. Looking forward to applying this knowledge to further enhance my work and contribute to innovative projects. 🚀 #LiquidChromatography #SCIEX #AnalyticalChemistry #ProfessionalDevelopment #ContinuousLearning

Labtop is proud to introduce the first large volume 3-tier orbital shaker in India, designed and manufactured under the "Make in India" initiative. This innovative shaker has the capacity to mix 700 flasks of 250 ml each, making it an ideal solution for large-scale laboratory operations. A laboratory shaker is a device used to mix, blend, or agitate substances in containers such as flasks, test tubes, or beakers. Shakers are commonly used in laboratories for various purposes, including: #MixingSolutions, #CulturingMicroorganisms #DissolvingSolids #ChemicalReactions #SamplePreparation #EnzymeReactions #Lifesciences #biopharma #Biotechnology #researchanddevolopment #Biology #university #pharmaceuticalindustries

✨I have successfully completed a course on "Birth of a drug", focusing on the use of organic chemistry in new drug development. 🌱💊 #organicchemistry #drugdevelopment #professionaldevelopment #ContinousLearning

It was an honor to deliver a talk at the Biopharma Conclave that concluded yesterday. I spoke about two new tools that every biopharma researcher must have while optimizing a bioprocess: Spent media analysis and Genome-Scale Modeling. This new approach allows us to optimize the process using just 4-6 trials compared to dozens of trials that are needed with conventional approaches. Spent media analysis monitors hundreds of metabolites in the spent media using mass spectrometric techniques such as LCMS and GCMS. The metabolic modeling then uses this data to provide deep insights into cellular metabolism. The metabolic simulations allow us to prioritize the experimental trials. #metabolomics #spentmediaanalysis #fermentation #BiopharmaConclave #Biosimilars #Biologics #CPHIConferences #InformaMarkets #CellAndGeneTherapy

Watch this short video to check on how we are revolutionizing the Drug Discovery landscape.

#Sample_Preparation Market Growth Soars Amid Rising Demand for Accurate Laboratory Testing >> https://lnkd.in/eYFmiP-c #Sample preparation refers to the process of #preparing a sample for analysis or testing in a #laboratory. It involves a series of steps designed to extract and prepare a #representative portion of a larger sample for #analysis. The goal of sample #preparation is to produce a sample that is #homogeneous, stable, and #representative of the original material, ensuring accurate and reliable results. Danaher Corporation Agilent Technologies, Inc. Illumina PerkinElmer QIAGEN Bio-Rad Laboratories Norgen Biotek Thermo Fisher Scientific Hamilton Company TECAN GROUP Promega Corporation Sartorius Sigma-Aldrich Biotage Waters Corporation Roche Applied Science #healthcareindustry #pharmaceuticalindustry #healthcareresearch #biotechnology #Samplepreparation #testing #laboratory #analysis

Contaminant Spot Check and Removal Assay (ContamSPOT) for Mass Spectrometry Analysis Here Smeriglio et al describes a novel method to check for and remove small contamination for peptide maping and proteomic analysis. As contaminations from detergents, salts, and polymers that compromise the data quality and can damage the chromatography and mass spectrometry instruments.   🛎 Overview: Smeriglio et al contamSPOT only requires one microliter of sample was used prior to MS injection to quantify contaminants by thin layer chromatography (TLC) plate (colorimetric or fluorometric assay)   Smeriglio et al believes they have developed a sensitive, rapid, and low-cost contaminant spot check and removal assay (ContamSPOT) Which can detect and quantify trace amounts of contaminants, such as detergents, salts, and other chemicals commonly used in MS sample preparation workflow.   🎯 Summary: ContamSPOT was then successfully applied to evaluate commonly used bottom- up proteomic methods, regarding the effectiveness of removing detergents, peptide recovery, 📌 Publication Link: https://lnkd.in/e_RCDaPj #scienceandtechnology #science #biopharmaceuticals #biotech #massspectrometry #massspec #chemistry #chemistrythatmatters #LCMS #mAb #proteomics #sciencecommunication #impurities