Plant BioProTech’s Post

Innovations on the horizon...it had to be S&G α-Amylases can be obtained from plants, animals and microorganisms. However, enzymes from fungal and bacterial sources have dominated applications in industrial sectors. The production of α-amylase is essential for conversion of starches into oligosaccharides. https://lnkd.in/djmK62Dw.

Our last paper is out! In it, we explore the creation of a "synthetic lichen" for CO2-driven bioproduction. For that, we used an engineered synthetic microbial community between a fungus (Y. lipolytica) and a marine cyanobacterium (Synechococcus). The product of the fermentation was the food aroma β-caryophyllene, naturally found in black pepper, rosemary, hops and cannabis.   https://lnkd.in/eJK9JV2g   How we made it: -             The cyanobacterium was engineered to produce and secrete sucrose from CO2 and light. -             The fungus was engineered to be able to use sucrose as feedstock. -             The fungus was engineered to produce a proof of concept product (β-caryophyllene) from sucrose. -             Culture conditions were optimised to allow them to grow together.   Why this is cool: -             We combined 1) the power of cyanobacteria to use CO2 and light – sustainable, abundant and desirable feedstocks with -             2) the metabolic versatility of fungi/yeast, which can be engineered to produce many compounds of interest (food, materials, fuels, pharmaceuticals, etc). -             Bonus: the co-culture was able to grow using sea water! Which is also abundant and desired for bioprocesses.   Next: This is just a proof-of-concept, inspired by previous works (e.g. Ducat’s lab). There is still a lot of room for optimisation to enable faster/better growth and production. Shout out to the people behind this work: Youngkyoung Park, Wenchao Chen, Lucie Studená, PhD, David Bell, Piotr Hapeta, Jing Fu, Peter Nixon.   #BezosCentreForSustainableProtein #MicrobialFoodHub Imperial College London #SyntheticBiology #PrecisionFermentation, #MetabolicEngineering, #SyntheticMicrobialCommunities, #CO2capture, #SyntheticLichen, #SustainableBioproduction

#Enzymes💚 exist in all of nature – in microorganisms, plants, animals and humans – and have been used for thousands of years to make bread, cheese, beer and wine. All enzymes produced today have a biological origin, via #fermentation or #extraction. 💡 This means there is a wide variety of enzymes used for specific functions and providing numerous benefits in many food, feed and technical applications. #EnzymesAreMiracles #Biotech #Innovation #Biotechnology #Bioeconomy

Plants are a rich source of valuable natural products, but it can be challenging to produce these products for commercial application. Organic synthesis is expensive and the biosynthetic pathways are often too complex to transfer to a microorganism. Published by Frontiers, this journal article explores how ‘hairy root’ cultures are now an attractive option as a production platform. Our platform is a great example of sustainable production for plants not suited to agricultural production. It offers genetic and biochemical stability alongside fast growth. And by taking plant-based production out of the field and into the factory, Samabriva offers a sustainable way to make high-value #biomolecules. Read the article here: https://lnkd.in/eq9BrYRn #biomanufacturing #bioprocessing #bioproduction #lifesciences

Water can be purified using mushroom substrate: the mixture of fungal filaments and horse manure that remains after harvesting mushrooms. The substrate effectively decreases concentrations of pesticides and drugs in contaminated water. Utrecht University researchers Brigit Van Brenk, Han Wösten, and colleagues demonstrate this in a paper in the scientific journal Applied Microbiology and Biotechnology. The results show the potential of the substrate as a promising alternative to current water purification methods. "It is a relatively straightforward, cost-effective, and sustainable way to purify water." Brigit Van Brenk #uuscience #research #mushrooms #water #purification

Happy to share this easy summary of my latest article!

How do food processing methods impact the stability of polyphenols? First talk of #PolyphenolsApplications2024 day 2 by Jianbo Xiao, Universidade de Vigo, Spain. Dr. Xiao highlighted: 🔸 Polyphenols, found in both natural and processed foods, are highly susceptible to degradation. 🔸 Common processing techniques such as thermal treatment and fermentation can significantly impact their stability. 🔸 Factors like pH, temperature, light, and oxygen play crucial roles in polyphenol stability. 🔸 Structural changes during processing can alter their bioactivity. 🔸 Emerging technologies offer solutions to enhance polyphenol stability in food products. #PolyphenolsWorldCongress #PolyphenolsApplications #foodscience #innovation #bioactives

📃Scientific paper: Bioflocculation of pollutants in wastewater using flocculant derived from Providencia huaxiensis OR794369.1 Abstract: Background Water pollution has become a major environmental and health concern due to increasing population and industrialisation. Microbial flocculants are promising agents for treatment of contaminated water owing to their effectiveness, eco-friendliness, and high biosafety levels. In this study, culture conditions of Providencia huaxiensis OR794369.1 were optimised and its bioflocculant was extracted, characterised and used to treat wastewater. Results The maximum flocculating activity of 92% and yield of 3.5 g/L were obtained when cultivation conditions were: 3% inoculum size, starch, casein, initial pH of 6, cultivation temperature of 30 ^oC and 72 h of fermentation. The bioflocculant is an amorphous glycoprotein biomolecule with 37.5% carbohydrates, 27.9% protein, and 34.6% uronic acids. It is composed of hydroxyl, amino, alkanes, carboxylic acid and amines groups as its main functional structures. It was found to be safe to use as it demonstrated non-cytotoxic effects on bovine dermis and African green monkey kidney cells, illustrating median inhibitory concentration (IC_50) values of 180 and > 500 µg/mL on both cell lines, respectively. It demonstrated the removal efficiencies of 90% on chemical oxygen demand (COD), 97% on biological oxygen demand (BOD) and 72% on Sulphur on coal mine wastewater. It also revealed the reduction efficacies of 98% (COD) and 92% (BOD) and 70% on Sulphur on domestic wastewater. Conclusion The bioflocculant was effective in reduc... Continued on ES/IODE ➡️ https://etcse.fr/SRcZ ------- If you find this interesting, feel free to follow, comment and share. We need your help to enhance our visibility, so that our platform continues to serve you.

📃Scientific paper: Bioflocculation of pollutants in wastewater using flocculant derived from Providencia huaxiensis OR794369.1 Abstract: Background Water pollution has become a major environmental and health concern due to increasing population and industrialisation. Microbial flocculants are promising agents for treatment of contaminated water owing to their effectiveness, eco-friendliness, and high biosafety levels. In this study, culture conditions of Providencia huaxiensis OR794369.1 were optimised and its bioflocculant was extracted, characterised and used to treat wastewater. Results The maximum flocculating activity of 92% and yield of 3.5 g/L were obtained when cultivation conditions were: 3% inoculum size, starch, casein, initial pH of 6, cultivation temperature of 30 ^oC and 72 h of fermentation. The bioflocculant is an amorphous glycoprotein biomolecule with 37.5% carbohydrates, 27.9% protein, and 34.6% uronic acids. It is composed of hydroxyl, amino, alkanes, carboxylic acid and amines groups as its main functional structures. It was found to be safe to use as it demonstrated non-cytotoxic effects on bovine dermis and African green monkey kidney cells, illustrating median inhibitory concentration (IC_50) values of 180 and > 500 µg/mL on both cell lines, respectively. It demonstrated the removal efficiencies of 90% on chemical oxygen demand (COD), 97% on biological oxygen demand (BOD) and 72% on Sulphur on coal mine wastewater. It also revealed the reduction efficacies of 98% (COD) and 92% (BOD) and 70% on Sulphur on domestic wastewater. Conclusion The bioflocculant was effective in reduc... Continued on ES/IODE ➡️ https://etcse.fr/SRcZ ------- If you find this interesting, feel free to follow, comment and share. We need your help to enhance our visibility, so that our platform continues to serve you.

The #futuretrends in #oleuropeinproduction are expected to be influenced by several key factors: #EmergingTechnologies: Advancements in biotechnology and nanotechnology may revolutionize extraction methods. For instance, research is exploring the use of ultrasound-assisted maceration to enrich olive oil with oleuropein. Additionally, green technologies like ohmic heating are being studied for their potential to extract oleuropein more efficiently and sustainably. #Sustainability and #Environmental Impact: There is a growing focus on sustainable production methods that minimize environmental impact. This includes the use of eco-friendly solvents and energy-efficient processes. The use of olive mill waste to extract oleuropein is an example of upcycling a byproduct into a valuable compound. #EconomicViability: Market demand, production costs, and regulatory requirements will significantly influence the economic viability of oleuropein production. The global oleuropein market is projected to grow, with factors like the increasing demand for natural health products and the compound's potential applications in various industries driving this growth. #Regulatory Compliance: As the market for oleuropein expands, so will the need for stringent regulatory compliance to ensure the safety and quality of products. This includes adherence to global safety and quality standards. #Market Expansion: The market for oleuropein is anticipated to expand, driven by increasing applications in the food and pharmaceutical sectors. This expansion will likely stimulate further investment in research and development to support production scale-up. #Research and #Development: Ongoing research will continue to uncover the potential health benefits of oleuropein, potentially leading to new applications and increased demand. #SupplyChain #Optimization: To ensure a consistent supply of raw materials, such as olive leaves, there will be a focus on optimizing the supply chain. Investment in #Infrastructure: Meeting the growing demand for oleuropein will necessitate investments in infrastructure, including the establishment of more extraction plants and upgrading of existing facilities. #GlobalMarketAnalysis: Companies will rely on global market analysis to identify opportunities for expansion and to tailor production to regional demands. https://lnkd.in/gCS6HgDN