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New publication! Rapid topographic margin assessment for breast tumors How can surgeons decide if a breast tumor has been completely removed? Alex Czaja, Matt Zacchary Blanco, Alice Jiang, Olga Eremina and Cristina Zavaleta at University of Southern California demonstrate a novel workflow: ⚗ Immunostaining using 26 SERS nanoparticles types 🔬 Topographic surface imaging using Raman microscopy This method could be used on freshly excised tissue to ensure complete resection during surgery. Read the full paper here: https://lnkd.in/esi2yNZB A Renishaw RA816 Biological Analyser was adapted for this work. #RenishawRaman #RamanSpectroscopy #Raman #ramanintissue

  • If a breast cancer lumpectomy is resected with unfavourable positive margins, traces of malignant tissue are likely on the specimen’s surface. Detecting and locating its presence while the patient is still in the OR could rapidly inform the surgical team where additional tissue should be removed to achieve a more complete excision. SERS nanoparticles consisting of different reporter molecules and chemically conjugated with biomarker protein-targeting moieties can serve as multiplexed spectral imaging contrast agents. A freshly excised specimen could be incubated with a cocktail of such nanoparticles, rinsed with fresh buffer to wash away unbound material, and topographically imaged. The resulting spectral and spatial information could be rendered in such a way to inform a surgical team whether any positive margins exist and where to remove additional tissue from the surgical cavity.

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