SEED Biosciences’ Post

More and more scientists from Academia and Pharma industries are using new approach methods (NAMs) to fast-forward the development of the next generation of therapeutics. This is why I am looking forward to next Wednesday, I will be attending with Meilin Berkhoff a great event, to connect experts with better tools for their translational research. I am particularly curious to hear more about how Organoid-based models are already impacting the 3Rs (Replace, Reduce, and Refine) initiative. Exciting to see that Adult Stem Cell-derived Organoids will also be a key element of the day. https://lnkd.in/e2S-m5V2 #Mimetas #Organoids #OrganonaChip #OoaC

Did you know that you can culture iPSC differentiated retinal organoids in our ClinoStar system? In this publication the #UniversityofIowa demonstrate how partial dissociation can be used to exploit the structure of iPSC-derived retinal organoids to produce highly pure photoreceptor cells without using specialized equipment or reagents such as antibody tags. #RetinalOrganoids #PhotoreceptorCells #StemCellResearch #Biotechnology #CellCulture #ResearchInnovation https://lnkd.in/eK3RkS3J

🌟 News in Circular RNA Research! 🌟 Embarking on the frontier of circular RNA-based translational studies? Our recent review, "Encapsulating In Vitro Transcribed circRNA into Lipid Nanoparticles Via Microfluidic Mixing," is your ultimate guide to groundbreaking discoveries! Its a guidance for researchers navigating the complex world of circular RNA. Imagine a roadmap leading you to successful encapsulation techniques using lipid nanoparticles (LNPs) — that's precisely what this review offers! Dive into the depths of scientific methods and techniques essential for the formulation process. From particle characterization to downstream processing of circ-LNPs, every crucial detail is meticulously covered, ensuring nothing stands between you and scientific advancement. Learn the art of producing in vitro transcribed circular RNA-containing LNPs, utilizing both commercially available lipid mixes and single components paving the way for groundbreaking discoveries in RNA therapeutics. With a step-by-step guide for initial in vitro verification, you will get a lot of precise details for moving forward with circRNA discoveries. Don't miss out on this transformative journey. Embrace the future of RNA research today! 🚀🔬 https://lnkd.in/eStWnNBx

𝐇𝐚𝐫𝐧𝐞𝐬𝐬𝐢𝐧𝐠 𝐭𝐡𝐞 𝐏𝐨𝐰𝐞𝐫 𝐨𝐟 𝐈𝐦𝐦𝐮𝐧𝐞 𝐂𝐞𝐥𝐥𝐬: 𝐄𝐧𝐠𝐢𝐧𝐞𝐞𝐫𝐢𝐧𝐠 𝐟𝐨𝐫 𝐓𝐡𝐞𝐫𝐚𝐩𝐞𝐮𝐭𝐢𝐜 𝐁𝐞𝐧𝐞𝐟𝐢𝐭 The immune cell engineering market refers to the dynamic and rapidly evolving field within biotechnology that focuses on the manipulation and enhancement of immune cells to treat various diseases, particularly cancer. This innovative approach involves modifying the genetic makeup or functional properties of immune cells, such as T cells, to bolster their ability to recognize and eliminate targeted cells, including cancerous ones. With the advent of cutting-edge technologies like CRISPR-Cas9 and advancements in cell therapy, the immune cell engineering market holds great promise for developing personalized and highly effective treatments, ushering in a new era in the realm of immunotherapy. 𝗥𝗲𝗾𝘂𝗲𝘀𝘁 𝗦𝗮𝗺𝗽𝗹𝗲:https://lnkd.in/dFnVXRh5 𝐏𝐫𝐨𝐦𝐢𝐧𝐞𝐧𝐭 𝐏𝐥𝐚𝐲𝐞𝐫𝐬 𝐢𝐧 𝐭𝐡𝐞 𝐈𝐦𝐦𝐮𝐧𝐞 𝐂𝐞𝐥𝐥 𝐄𝐧𝐠𝐢𝐧𝐞𝐞𝐫𝐢𝐧𝐠 𝐌𝐚𝐫𝐤𝐞𝐭 Thermo Fisher Scientific Merck KGaA, Darmstadt, Germany Danaher Corporation Lonza Miltenyi Biotec Takara Bio USA, Inc. FUJIFILM Irvine Scientific Agilent Technologies Bio-Techne Bio-Rad Laboratories Becton Dickenson Charles River Laboratories Creative Bioarray Sartorius Novogene America AGC Biologics #ImmuneEngineering #CARTCells #TCRTherapy #ImmuneTherapy #CancerImmunotherapy #GeneEditing #Immunomodulation #CellTherapy #ImmunotherapyResearch #CellularEngineering

In vivo production of CAR-T cells using virus-mimetic fusogenic nanovesicles

🚀 Logic-gated cells are revolutionizing medicine by precisely targeting specific tissues, pushing the boundaries of what's possible in therapy! 🔬 In proof-of-concept experiments, Sara Martire et al., successfully identified receptors that function as NOT gates when paired with activating receptors. 💡 This approach not only speeds up the discovery process but also generates large datasets to train machine learning models, optimizing the function of logic-gated cell therapeutics. ⚓ Remember: in flow cytometry, the only thing flowing faster than the cells is our excitement! #FlowCytometry #Innovation #CellTherapy #FlowCytometry #MachineLearning

Today in "how to improve RNA delivery into cells beyond what we could have expected": The wonders of liquid crystalline inverted lipid phases In the realm of RNA therapeutics, overcoming the challenge of efficient intracellular delivery has been "the big obstacle" that most papers talk about. Once lipid nanoparticles (LNPs) have entered the cells, disruption of the LNP structure and endosomal membrane is crucial for sufficient cytosolic RNA delivery. However, in this process the majority (≥98%) of RNA molecules delivered remain trapped inside endosomal and lysosomal compartments, leading to degradation or efflux out of the cell. Suck a (tragic) problem is mostly looked from the point of view of either endosomes/RNA or LNPs/RNA by themselves. However, looking at this problem from a structure-activity relationship might have just rendered some of the most relevant findings of the year so far. This paper presents the instructions for a bottom-up rational design of LNPs with defined lipid superstructures encapsulating siRNA. Then, by combining pretty fancy techniques (such as cryoTEM and SAXS), they have identified and characterized defined crystalline lipid-RNA structures in the core of LNPs, and made quite some fascinating findings: 1) For once, the study demonstrates that LNPs with liquid crystalline inverse hexagonal phases exhibit significantly higher intracellular silencing efficiency compared to conventional lamellar LNPs. 2) Unlike lamellar LNPs, which undergo an in situ phase transition upon interaction with anionic membranes, LNPs with pre-programmed hexagonal phases offer a more streamlined one-step delivery mechanism, enhancing their therapeutic potential. To me, the biggest highlight is that through inducing pre-programmed lipid phases, transfection efficiency can be enhanced. Since the formation of non-lamellar lipid structures is driven by total lipid composition and not exclusively on specific ionizable lipids, the here presented approach for inducing inverse hexagonal structures can be retrofitted to LNPs with other components to increase their LNP potency. If you want to know more, the article is open access: https://lnkd.in/eTqkZ__3 #lipidnanoparticles #nanotechnology #nanomaterials #mechanism #delivery #genedelivery #drugdelivery #rna #mRNA #lnps #nanoparticles

𝐔𝐧𝐥𝐨𝐜𝐤 𝐍𝐞𝐱𝐭-𝐆𝐞𝐧 𝐀𝐧𝐭𝐢𝐛𝐨𝐝𝐲 𝐓𝐡𝐞𝐫𝐚𝐩𝐢𝐞𝐬! Join us for an exclusive webinar on "Enhancing Next-Gen Antibody Therapies: Advanced Strategies for Fcγ Receptor Binding Assays" on 𝟐𝟑𝐫𝐝 𝐉𝐮𝐥𝐲, 𝐓𝐮𝐞𝐬𝐝𝐚𝐲 𝐚𝐭 𝟓 𝐏𝐌 𝐂𝐄𝐓.. Discover the cutting-edge techniques for Fcγ receptor binding studies, crucial for optimizing IgG1, IgG2, and IgG4 monoclonal antibodies. 𝐊𝐞𝐲 𝐓𝐚𝐤𝐞𝐚𝐰𝐚𝐲𝐬: 1. Deep dive into IgG-Fcγ receptor interactions. 2. Best practices for assay design and development. 3. Real-time, label-free analysis using the Octet® BLI Platform. Don’t miss insights from Dr. Stuart Knowling, a leading biophysics expert! 👉 𝐑𝐞𝐠𝐢𝐬𝐭𝐞𝐫 𝐧𝐨𝐰: https://lnkd.in/gP8cE7hY #AntibodyResearch #FcγReceptors #DrugDevelopment #Biophysics #MonoclonalAntibodies #OctetBLI #Webinar #AssayDevelopment #PharmaScience #Biotech #ProteinAnalysis #Research #Immunotherapy #Biosimilars #Biopharma

𝐔𝐧𝐥𝐨𝐜𝐤 𝐍𝐞𝐱𝐭-𝐆𝐞𝐧 𝐀𝐧𝐭𝐢𝐛𝐨𝐝𝐲 𝐓𝐡𝐞𝐫𝐚𝐩𝐢𝐞𝐬! Join us for an exclusive webinar on "Enhancing Next-Gen Antibody Therapies: Advanced Strategies for Fcγ Receptor Binding Assays" on  𝟐𝟑𝐫𝐝 𝐉𝐮𝐥𝐲, 𝐓𝐮𝐞𝐬𝐝𝐚𝐲 𝐚𝐭 𝟓 𝐏𝐌 𝐂𝐄𝐓.. Discover the cutting-edge techniques for Fcγ receptor binding studies, crucial for optimizing IgG1, IgG2, and IgG4 monoclonal antibodies. 𝐊𝐞𝐲 𝐓𝐚𝐤𝐞𝐚𝐰𝐚𝐲𝐬: 1. Deep dive into IgG-Fcγ receptor interactions. 2. Best practices for assay design and development. 3. Real-time, label-free analysis using the Octet® BLI Platform. Don’t miss insights from Dr. Stuart Knowling, a leading biophysics expert! 👉 𝐑𝐞𝐠𝐢𝐬𝐭𝐞𝐫 𝐧𝐨𝐰: https://lnkd.in/gP8cE7hY #AntibodyResearch #FcγReceptors #DrugDevelopment #Biophysics #MonoclonalAntibodies #OctetBLI #Webinar #AssayDevelopment #PharmaScience #Biotech #ProteinAnalysis #Research #Immunotherapy #Biosimilars #Biopharma

Have a look at the article about tumorspheres, emphasize the importance of employing #3Dcellculture models and understand how #VolumeEM with serial block face brings value in their research, drug discovery, and therapeutic studies. Read the full paper to see more of their beautiful ultrastructural work and in-depth analyses detailing the important differences between 3D cultured cells and traditional monolayers.