Twist Bioscience’s Post

Immuno phenotyping and viability never felt this good! Simple, fast and robust with added benefits like apoptosis as well as transfection efficiency. At Revvity we have many tools to help your research workflows and the Cellaca PLX is just one of them! #cellandgenetherapy #celltherapy #celltherapymanufacturing #flowcytometry #biotechnology

We are excited to share with you some images from our lab of cell spheroids. Spheroids and organoids are culturing models which better imitate tumor and tissue in-vivo state, as compared to the traditional 2D monolayer cell culture. Spheroids exhibit the following features: 3D multicellular structure comprised by a network of cell-to-cell and cell-to-matrix interactions, similar to in-vivo tissue structure. Gene expressions which more closely resemble expression profiles of the respective tumor tissue in-vivo. Mimicking of tumor spatial organization - outer layer of highly proliferating cells, middle region comprising senescent cells and a core containing necrotic dead cells. Replicating the tumor microenvironment involves mirroring gradients of metabolites, oxygen, pH, and nutrients from the outer layer to the core. Enhanced complexity may be attained through co-culturing of tumor cells alongside other cell types from tumor microenvironment. It is now generally accepted that from physiological point of view, in many cases in comparison to mono layered culture, spheroids better represents solid tumor and it is advisable to use them for anti-cancer drug discovery and early stage development! #cell #spheroids #laboratory #3d

📢#FBL-Vol. 28 No. 3 Proteomic Study on Multi-Organ Metastases of Human Ovarian Clear Cell Carcinoma Cell Line in a Xenograft Mouse Model Based on a Novel Sequence-Specific Analysis Strategy https://lnkd.in/geSZWQe5 🔓#OpenAccess #CellBiology #Biochemistry #MolecularBiology #CellularBiology

If you or a group you know is interested in detecting RNA Fusions, please share this study demonstrating the utility of Twist's Exome Capture-based RNA-sequencing Assay to detect recurring and novel fusions.

#Citation 🔊Scientists found a new way tumors present signals for advanced cancer treatment using an innovative sequencing method and focusing on specific immune system markers. #Genscript, through their library service, provided crucial synthetic peptides for the study, supporting the identification of novel, immunogenic, non-canonical peptide sequences in primary tumor tissue from human cervical tumor samples. Cancer-specific peptides were identified without relying on genomic or RNA data. The integrated approach, using MHC binding rank, confidence scores, and retention time prediction, resulted in almost a 2-fold improvement in spectral assignments. The machine learning model MARS achieved an estimated accuracy of over 85.7%. Read more here: https://lnkd.in/eYY9_xej

Interested in detecting RNA fusions, but not sure where to start? This publication "An Exome Capture-Based RNA-Sequencing Assay for Genome-Wide Identification and Prioritization of Clinically Important Fusions in Pediatric Tumors" demonstrates the utility of Twist Bioscience Exome for RNA-seq and fusion detection! Authors developed, tested, and validated (1) Twist exome with RNA from fresh, frozen, or formalin-fixed samples from patients with pediatric hematologic malignancies and solid tumors and (2) a gene fusion detection assay that employs four fusion callers (Arriba, FusionCatcher, STAR-Fusion, and Dragen). The new bioinformatics platform detects fusions, prioritizes them, and custom curates downstream processes for consensus fusion calling with high accuracy and efficiency. News release: https://lnkd.in/gynBQvnm #RNAexome #RNAfusion #transcriptomics

💥 New Publication Alert---   💡 Title: Conceptual breakthroughs of the long noncoding RNA functional system and its endogenous regulatory role in the cancerous regime   🧐 Authors: Anyou Wang   📂 This review focuses on big data studies that break through the critical concepts of the lncRNA functional system and its endogenous regulatory roles in all cancers.   Curious to know more? Dive into the article: ➡ https://lnkd.in/gCAANafU   #bigdata, #LongNoncodingRNA, #noncodingRNA, #cancer, #function, #endogenous, #regulatory role

If you or a group you know is interested in detecting RNA Fusions, please share with them this study which utilizes a Twist exome capture-based RNA-sequencing assay to detect recurring and novel fusions.

A #biobank is a specialized repository that facilitates systematic collection, storage, and management of a wide variety of biological samples, such as tissues, blood, DNA, and other biomaterials, along with associated clinical and demographic information from individuals. These samples are preserved under controlled conditions, often at extremely low temperatures, to maintain their integrity for future research and medical purposes. Biobanks play a crucial role in #biomedical research by providing a valuable resource of well-annotated biospecimens for studying diseases, understanding their underlying mechanisms, and developing personalized treatments. To learn more: https://lnkd.in/dyqHp_yf #Biobanking #Biorepository #Cryopreservation #BiomedicalResearch #PrecisionOncology #Biopreservation #Storage #Cancer #CancerResearch #Multiomics #Databank #Biotechnology #Oncomedicine #CancerBiomarker The Scientist

❓ Question: What are voxels? 💡 Answer: Voxels are an area of tissue that contain cells, genes from those cells and microorganisms, and the stromal context of proteins (collagen), glycans, metabolites, and peptides, all identified in the 3D space within a tumor tissue. You can read more about voxels and the tumor microenvironment from our most popular publication: https://lnkd.in/gK3rRkDK Sammy Ferri Borgogno, Jared K. Burks, Erin Seeley, Trevor McKee, Danielle Stolley, Akshay Basi, Javier Gomez, Shamini Ayyadhury, Melinda Yates, Mok Samuel C, Lunaphore, STOmics, Visiopharm, Bruker #SpatialBiology, #Multiomics, #Multiplexing