Aragen Life Sciences’ Post

【Biology/Environment】 Enhancing Recombinant Protein Expression in Lettuce Recombinant proteins are crucial in pharmaceutical and other industries. A production method uses plants as hosts in transient protein expression systems. Researchers at the University of Tsukuba developed a technique to increase recombinant protein expression in lettuce by inhibiting RNA-dependent RNA polymerase. This technique achieved the highest protein expression levels reported in lettuce to date and is a promising approach for large-scale protein production. Read more details here; https://lnkd.in/eJyXzwv8 Original Paper; https://lnkd.in/e5-jeC7c

Episomal stable pools in WAVE bioreactors for fast and robust protein production   Recombinant proteins are important resources in several stages of drug discovery as well as many other types of research. The amounts of pure protein required span from a few micrograms for immunisations to hundreds of milligrams or even grams for activity evaluations and structure determinations. Here a highly scalable process for protein production in CHO cells that generates robust product quality and high performance throughout scales is presented.   Recurrent production of large amounts of protein is usually done by generating stable cell lines from high-producing clones. In drug discovery and other projects focusing on multiple targets this will become costly and time-consuming. In this study a highly scalable protein production platform that utilizes the same technology for transient expression and stable pools is presented. Transient expression is used for small-scale production and the QMCF technology from Icosagen is applied to large-scale recombinant protein production in WAVE bioreactors to generate episomal stable pools in suspension cell culture.  This shortens timelines for production scaling since proliferation can be performed simultaneously as antibiotic selection. The large-scale episomal pool protocol was compared to the transient expression protocol in terms of production titers for six human secreted proteins. The results show a great improvement in purified protein/ml culture for most of the targets, while the rest showed similar amounts using both protocols.   The platform is shown to deliver a seamless and efficient scale-up with robust product quality, while keeping the flexibility in the development process, and could therefore be considered as a suitable production platform for supplying reagents to lead optimization, assays, and initial animal experiments in early development/drug discovery. https://lnkd.in/dkCQrAzV  

Introduction of Gene-Biocon Heat-stable Trypsin Solution. Gene-Biocon has launched a Heat-stable Trypsin Solution product currently. The product was named TrypRE select (1×). The TrypRE select (1×) is a recombinant thermostable trypsin digestion solution produced by fermentation, purification, and formulation from a genetically engineered strain of Pichia Pastoris. TrypRE select is a non-animal origin recombinant enzyme suitable for dissociating various adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes and embryonic stem cells. The recombinant enzyme used in this product is a trypsin-like protease from Fusarium oxysporum, a serine protease that can selectively hydrolyze the peptide chain composed of the carboxyl end of lysine or arginine in proteins, exhibiting enzymatic properties identical to those of recombinant porcine and bovine trypsin. #trypsin #recombinant #biologcial #biotechnology https://lnkd.in/gcBNWznN

We are excited to launch our series on Protein Sciences+ at Dalriada Drug Discovery!  At Dalriada Drug Discovery we care as much as you do about obtaining highest quality and truly fit-for-purpose protein to enable discovery programs from Hit ID to PDC. Why? While the therapeutic modalities are undergoing rapid innovation (TPD, Covalency, XTAC), protein assays and proteins screens remain at the heart of target-oriented drug discovery.  We ourselves rely on our own protein production department to successfully enable the downstream applications in crystallography, ITC, SPR, covalent MS screens, DSLS, DSF, MST, BLI, and a wide array of biochemical assays. At Dalriada, we don’t just care about delivery of required protein, we care about the protein delivered to be truly fit-for-purpose to enable a program to successful PDC nomination.   Our North American team, with deep roots from Structural Genomics Consortium, has over the years delivered dozens of recombinant protein and protein complexes with successful downstream applications and breaking published and previously obtained records in yield and quality.  We are excited to announce that we have made significant investments recently in expanding our capacity across all protein production platforms including bacterial, insect, mammalian, and protein membranes, which brings us again to the new heights of supporting stand-alone protein production programs as well as integrated discovery programs.  If you have an upcoming need in protein sciences/assays/screening, don’t hesitate to reach out to us here https://lnkd.in/g23nGskJ.  Look forward to sharing more in our next post on Protein Sciences+ Dalriada Drug Discovery #ProteinSciences #DrugDiscovery #ProteinProduction #HITID

What is a “cell-free” protein synthesis system? The cell-free protein synthesis (CFPS) system is a technology that can express target proteins quickly and with high throughput. It uses exogenous DNA or mRNA as a template and is passed through the cell lysate under the action of a variety of enzymes. Supplement substrates and energy substances to achieve protein synthesis in vitro. At present, the CFPS system mainly includes four systems: Escherichia coli lysate, rabbit reticulocyte lysate, wheat germ extract, and yeast extract. Escherichia coli lysate is a relatively complete prokaryotic CFPS system that can achieve high-throughput expression of proteins, but it has certain limitations in post-translational modification of functional proteins. Rabbit reticulocyte lysate is a eukaryotic protein synthesis platform developed based on mammalian systems. Additional micrococcal nuclease is required to achieve functional protein expression. Wheat germ extract is a relatively complete eukaryotic system and is suitable for high-throughput synthesis of complex proteins. However, the high preparation cost limits large-scale synthesis. Yeast extract can achieve post-translational modification of proteins, and the preparation process is simple, but the yield is low. At present, most researchers still use E. coli lysate, especially the PURE (protein synthesis using recombinant elements) system to achieve protein synthesis. #cellculture #biopharma #biotech #biotechnology #bioengineering #cellfree #Ecoli #yeast #fermentation https://lnkd.in/gcBNWznN

🚀 Enhancing Perfusion Cultivation with L-Arginine 🚀 It is time for the Monday's routine. Today's publication gives - like it often happens - insights about how small changes in media composition may have an significant influence on recombinant production in CHO. The study explored the impact of high L-Arginine concentrations on CHO cell perfusion cultures with promising results: 🧪 Optimal L-Arginine Concentration At a concentration of 40 mM, L-Arginine was shown to enhance perfusion filter transmission with CHO cells adapting to supplementation resulting in stable cell viability. Under these conditions, protein production was elevated while reducing degradation rates. 📈 Increased Transmission of mABs L-Arginine improved the transmission of recombinant domain antibodies through the perfusion filter, a key factor in maintaining high productivity and minimizing product degradation. ⚖️ Viability & Timing: Concentrations above 40 mM reduced cell viability, leading to unwanted product degradation. However, maintaining an optimal balance at 40 mM preserved the cell culture health over longer periods, when supplemented at the right time point (day 7) of perfusion culture. 💡 L-Arginine concentration is a suitable point for adjustments in CHO perfusion media optimization making continuous manufacturing processes more efficient. However, further experiments should be performed to evaluate scalability between the tested 5 L system and commercially common production scales. Find the publication here: https://lnkd.in/eDpezZVz #ChineseHamsterOvary #Biomanufacturing #ProteinProduction #PerfusionCulture

Understanding Recombinant Enzymes and Their Significance. Recombinant #enzymes are derived from genetically modified organisms (GMOs) and possess the ability to cut splice join, and replicate DNA molecules with remarkable precision. By harnessing these enzymes, scientists can manipulate genetic material with unprecedented accuracy, amplifying their impact on various industries. From medical research to agriculture, recombinant enzymes have paved the way for groundbreaking advancements. #enzymes #biology #biological #biosciences #geneticengineering #bioengineering #biopharmaceutical #biomedical https://lnkd.in/gcBNWznN

Promotion: LAB-A-PORTER x OriGene. Use Promo Codes To Redeem Your Offer Today! To support your research needs on recombinant proteins, OriGene and LAB-A-PORTER are thrilled to announce a special promotion for selected recombinant proteins! From now until 31 March 2024, for purchase of MVPro recombinant proteins from OriGene, you can receive 20% off. Limited offer. Grab the chance to stock up on some recombinant proteins! Use promo code: MVPRO20 to redeem your offer today! T&C applies. All products listed on this site and sold through LAB-A-PORTER Limited are for research use only (RUO). Not for human use. Not for therapeutic or diagnostic use. Learn more: https://lnkd.in/gbaewUMy

What is the future of microbial expression systems? 🦠 Microbial expression systems are widely used to manufacture diagnostic and therapeutic proteins. However, the commercialization success rate for new drug developments is less than 3%, partly due to high development and manufacturing costs, long turnaround times, and challenging product safety profiles. 💸 CDMOs, including KBI, are jumping in to update microbial expression systems to surpass the modest yields of traditional E. coli models. KBI Biopharma has developed PUREcoli, a microbial expression system incorporating over 1,000 gene edits to enhance protein production. 📈 Learn more about it in our latest article! ⬇️ https://lnkd.in/d-KT-xbg #biotechinnovation #proteinproduction #therapeuticproteins #CDMOs #drugdevelopment #biomanufacturing #biotechresearch #biotechindustry

I am excited to share that earlier this month my first author paper on a part of my PhD research in the field of biologics manufacturing process development conducted at The Johns Hopkins University was published in Metabolic Engineering Journal. In this study, we elucidate the fate of dipeptides in mammalian cell cultures used for biologics manufacturing through an integration of stable isotope (13C) labeling and kinetic modeling. As biologics manufacturing processes have transitioned from traditional fed-batch to intensified fed-batch processes and continuous modes of manufacturing, a higher demand for critical amino acids will be required. Indeed, dipeptides can serve as alternatives to sparingly soluble and unstable amino acids in cell culture basal media and feed in future media designs. Gaining a deeper understanding of dipeptide metabolism could help deliver these nutrients efficiently and improve bioprocess performance. Thanks to Advanced Mammalian Biomanufacturing Innovation Center for funding this research. https://lnkd.in/dynj8XiQ #biotechnology #pharmaceuticals #biomanufacturing #healthcare #upstream #processdevelopment #cellculture