Zhuhai Gene-Biocon Biological Technology Co., Ltd.’s Post

Introduction of Gene-Biocon Heat-stable Trypsin Solution. Gene-Biocon has launched a Heat-stable Trypsin Solution product currently. The product was named TrypRE select (1×). The TrypRE select (1×) is a recombinant thermostable trypsin digestion solution produced by fermentation, purification, and formulation from a genetically engineered strain of Pichia Pastoris. TrypRE select is a non-animal origin recombinant enzyme suitable for dissociating various adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes and embryonic stem cells. The recombinant enzyme used in this product is a trypsin-like protease from Fusarium oxysporum, a serine protease that can selectively hydrolyze the peptide chain composed of the carboxyl end of lysine or arginine in proteins, exhibiting enzymatic properties identical to those of recombinant porcine and bovine trypsin. #trypsin #recombinant #biologcial #biotechnology https://lnkd.in/gcBNWznN

Recombinant protein expression system - yeast expression system. According to the type of host cell, gene expression systems can be divided into prokaryotic expression systems, yeast expression systems, mammalian expression systems, and insect expression systems. Yeast is a eukaryotic microorganism whose cells have the ability to express foreign proteins and can reach a very high cell density state. Expressing foreign proteins in yeast can undergo post-translational modifications, such as phosphorylation and glycosylation. Since yeast host cells combine the excellent properties of bacterial and higher-order organism host cell expression systems, they are a good choice when foreign proteins cannot be successfully expressed in E. coli. Yeast strains used for foreign protein expression include Saccharomyces cerevisiae, Schizosaccharomyces pombe, Pichia pastoris, and Kluyveromyces lactis. The recombinant #trypsin, recombinant #lysyl endopeptidase, #Kex2 protease, #enterokinase, etc produced by Gene-Biocon are expressed by yeast. #cell #biology #biological #Biotechnology #bioengineering #geneticengineering #expression https://lnkd.in/gcBNWznN

Compared with other expression systems, the Pichia pastoris expression system has the following advantages: (1) Contains a unique and powerful AOX (alcohol oxidase gene) promoter, which can strictly control the expression of foreign genes with methanol; (2) The expression level is high, and it can be expressed intracellularly or secretedly. In Pichia pastoris, the highest reported expression level of tetanus toxin C is 12g/l, which is generally greater than 1g/l. Most foreign genes are expressed at higher levels than in bacteria, Saccharomyces cerevisiae, and animal cells. Generally, exogenous genes in Pichia pastoris contain signal peptide sequences that guide secretion, so that the expressed exogenous target protein can be secreted into the fermentation broth, which is beneficial to isolation and purification; (3) The fermentation process is mature and easy to scale up. There is already a fermentation process for large-scale industrial high-density production, and the dry cell weight reaches more than 100g/l. When expressing recombinant proteins, it has been successfully scaled up to 10,000 liters; (4) The cultivation cost is low and the products are easy to separate. The fermentation medium used by Pichia pastoris is very cheap. Generally, the carbon sources are glycerol or glucose and methanol, and the rest are inorganic salts. The medium does not contain protein, which is conducive to the isolation and purification of downstream products; while the inducers used by Saccharomyces cerevisiae are generally relatively expensive. of galactose; (5) The foreign protein gene is genetically stable. Generally, foreign protein genes are integrated into the Pichia pastoris chromosome, replicated along with chromosome replication, and are not easily lost; (6) As a eukaryotic expression system, Pichia pastoris has a eukaryotic subcellular structure and has post-translational modification processing functions such as glycosylation, fatty acylation, and protein phosphorylation. #cell #biology #biological #Biotechnology #bioengineering #geneticengineering #expression https://lnkd.in/gcBNWznN

Recombinant Enzymes in Genetic Engineering: An Overview. In #genetic #engineering, recombinant #enzymes serve as the molecular scissors that precisely cut and rearrange DNA sequences. The most widely used enzyme in this process is the DNA restriction endonuclease. This versatile tool allows scientists to selectively cleave DNA at specific recognition sites, facilitating the manipulation of genes. Another essential enzyme in the genetic engineering toolkit is DNA ligase, which efficiently seals the gaps created during DNA splicing. Together, these enzymes enable the creation of genetically modified #organisms and pave the way for countless scientific breakthroughs. #biology #biological #bioscience #bioengineering #geneticengineering #biopharmaceuticals #biopharma #proteins https://lnkd.in/gcBNWznN

In conclusion, recombinant #enzymes have emerged as pivotal catalysts of change in the field of biomedicine. With their immense potential and diverse applications, these enzymes have paved the way for groundbreaking advancements in therapeutics, diagnostics, and beyond. Through ongoing research and refinement the impact of recombinant enzymes in biomedical advancements is only expected to grow, ultimately leading to the development of more effective and accessible treatments for various diseases and medical conditions. #enzyme #bioengineering #biotechnology #biotechnologies #geneticengineering #biology #protein #genetic #biomedicalengineering #biomedical #biopharma #biopharmaceutical https://lnkd.in/gcBNWznN

Happy to share our recent book chapter in book entitled Microbial Products for Health and Nutrition https://lnkd.in/dFDRS3mC #Indrashiluniversity #IU #lifesciencesuniversity #GSBTM #Cadilapharmaceuticals #Departmentofbiosciences #SchoolofScience #Microbiology #Biotechnology

Promotion: LAB-A-PORTER x OriGene. Use Promo Codes To Redeem Your Offer Today! To support your research needs on recombinant proteins, OriGene and LAB-A-PORTER are thrilled to announce a special promotion for selected recombinant proteins! From now until 31 March 2024, for purchase of MVPro recombinant proteins from OriGene, you can receive 20% off. Limited offer. Grab the chance to stock up on some recombinant proteins! Use promo code: MVPRO20 to redeem your offer today! T&C applies. All products listed on this site and sold through LAB-A-PORTER Limited are for research use only (RUO). Not for human use. Not for therapeutic or diagnostic use. Learn more: https://lnkd.in/gbaewUMy

What is the future of microbial expression systems? 🦠 Microbial expression systems are widely used to manufacture diagnostic and therapeutic proteins. However, the commercialization success rate for new drug developments is less than 3%, partly due to high development and manufacturing costs, long turnaround times, and challenging product safety profiles. 💸 CDMOs, including KBI, are jumping in to update microbial expression systems to surpass the modest yields of traditional E. coli models. KBI Biopharma has developed PUREcoli, a microbial expression system incorporating over 1,000 gene edits to enhance protein production. 📈 Learn more about it in our latest article! ⬇️ https://lnkd.in/d-KT-xbg #biotechinnovation #proteinproduction #therapeuticproteins #CDMOs #drugdevelopment #biomanufacturing #biotechresearch #biotechindustry

Read this case study to understand how Aragen’s expert team of scientists helped its client obtain more than 80 mg of a mammalian secretory protein, whose expression in its soluble precursor form is considered challenging in recombinant protein expression systems. Aragen’s team redesigned the workflow to create a cost-effective, scalable process to achieve successful expression of the untagged protein in E. coli expression system and facilitate its subsequent in vivo maturation. The team optimized expression parameters to enhance yield of the recombinant protein’s mature form. We further refined and optimized the initial trial protocol provided by the client to meet standard quality and quantity requirements for the final product. Subsequently, our team redesigned the purification protocol to attain standard specifications in a scalable format with reduced turnaround time. This case study highlights Aragen's expertise, instrumental in reshaping the client's strategy and achieving a critical milestone. To read the whole case study click here https://lnkd.in/gHcgKmMa or write to us at info@arage.com #invivo #invitro #drugdiscovery #drugdevelopment #casestudy #lifesciences #endtoendsolutions #protein

Formulation and Characterization of Diclofenac Sodium Nanogel for Controlled Drug Release Read the Article here: https://bit.ly/3TTwUUM #Carbopol940 #Diclofenacsodium #Entrapmentefficiency #EudrgitS100 #Nanogel #biosciences #Nanoparticles #Science #NanoScience #technology #Biology #Bioengineering #Animalbiotechnology #Agriculturebiotechnology #Biochemistry #Bioethics #Bioinformatics #Biopolymer #Cellbiology #Forensicsciences #Immunology #IndustrialBiotechnology #Microbiology #Genomics #Proteomics #PharmaceuticalSciences