Dr. Amit Kumar’s Post

Introducing the 10x Genomics #XeniumPrime5K Assays: With 5,000-gene #Xenium assays, experience unrivaled performance and unparalleled turnaround time. Take the next step in your research journey and discover the power of #single-cellspatial imaging. Explore your biology like never before! 🧬🔬

Celebrating Innovation: The 2024 Nobel Prize in Physiology or Medicine 🧬 This year, the 2024 Nobel Prize in Physiology or Medicine honors Victor Ambros and Gary Ruvkun for their groundbreaking work on microRNAs. These small but mighty RNA molecules play a crucial role in controlling gene expression across all eukaryotic cells, impacting both animal and plant biology. At ACTV, this groundbreaking research resonates deeply with our commitment to innovation. Leveraging the regulatory power of microRNAs, we've developed a new generation of high-tech cosmetic bioactives with our patent pending ProliCell® technology. Our cutting-edge technology harnesses these small RNA genes to offer revolutionary skincare solutions that are not just effective but are also at the forefront of scientific advancement. Join us in recognizing this significant scientific milestone and explore how it influences our advanced cosmetic offerings. Discover the science that makes beauty sustainable and effective. #NobelPrize2024 #InnovationInSkincare #MicroRNAs #ACTV #ProliCell

October issue from #CellReports. See below to learn about the cover and featured content👇 and access the full issue here: https://lnkd.in/dyDaP62X On the cover: Navigating crevasses at night is aided by spatial learning and memory, which requires forms of synaptic plasticity such as long-term potentiation (LTP). In this issue of Cell Reports, Rumian et al. describe how the different phases of LTP are mediated by distinct CaMKII functions. The picture was taken on the slopes of Cotopaxi, an active stratovolcano in Ecuador, by Ulli Bayer. Featured content: 🔵 "Metabolite regulation of #epigenetics in #cancer" Shanghai Medical College of Fudan University Authors: Pu Wang, Lei-Lei Chen, Yue Xiong, Dan Ye 🔵 "Integration of #genetic and #chromatin modification data pinpoints #autoimmune-specific remodeling of enhancer landscape in CD4+ #Tcells" EMBL European Molecular Biology Laboratory, University of Tartu, Stanford University, GSK, Universidad de Málaga Authors: Neha Daga, PhD, Nila Servaas, Kai Kisand, Dewi Moonen, Christian Arnold, PhD, Armando R., Epp Kaleviste, Kingo Kulli, Reet Kuuse, Katrin Ulst, Lars Steinmetz, Pärt Peterson, Nikolina Nakic, Judith Zaugg 🔵 "Mitotic ER-mitochondria contact enhances #mitochondrial Ca2+ influx to promote #celldivision" Peking University, Kunming University of Science and Technology Authors: Gan Zhao, Mingkang Jia, Shicong Zhu, He Ren, Guopeng Wang, Guangwei Xin, Mengjie Sun, Xiangyang Wang, Qiaoyu Lin, Qing Jiang, Chuanmao Zhang

#CellReports is now on LinkedIn! Read the latest issue below.

🔬It's #FluorescenceFriday and today we celebrate another chapter in the history of #microscopy: 30 years ago, on the 2nd of June 1994, the prestigious journal #Nature published a paper titled "Implications for bcd mRNA localization from spatial distribution of exu protein in Drosophila oogenesis." 👩🔬Only few months after the first introduction of Green Fluorescent Protein (GFP) as a reporter for gene expression, Shengxian Wang and Tulle Hazelrigg from Columbia University published a ground-breaking paper, utilizing GFP to indicate the expression patterns of a protein called Exu, which is essential for the early development of fly embryos. The fusion product of both proteins, Exu and GFP, was functional in the fly cells and lead to a normal development of embryos, making it suitable to report on the expression of the protein in real time using a fluorescence #microscope. 🔬Since then, fluorescent proteins became a routine tool in #research labs around the world. In 2006, Nobel Prize laureate Eric Betizg from Howard Hughes Medical Institute (HHMI) Janelia Research Campus and his colleagues, first described Photoactivated Localization Microscopy (PALM), a #SuperResolution technique which uses fluorescent proteins and is compatible with live-cell imaging. Using PALM, scientists can perform #SingleMolecule experiments in live cells, including where other imaging methods are more challenging. 🎉🔬ONI is happy to celebrate such achievements in the #HistoryOfScience and is offering congratulations to the brilliant scientists who paved such remarkable paths in #LifeScience research.💐 Read the original historic paper at https://hubs.ly/Q02z0dFD0 #Nanoimager #dSTORM #PALM #innovation #biology #biotech #biotechnology #innovation #scienceinnovation #creativity #creativescience #GFP #reportergene #SMLM

📢 Conscience has released the results of CACHE Challenge #1, which aimed to predict "hits" for Parkinson's disease drugs through computational models. Conducted in partnership with The Structural Genomics Consortium (SGC) and funded by The Michael J. Fox Foundation for Parkinson's Research, the challenge has yielded seven promising discoveries for familial Parkinson's disease. The CACHE Challenge has two key outcomes: firstly, new discoveries that can help advance novel treatments for Parkinson’s disease, and secondly, prospective benchmarking of multiple computational methods to reveal the state-of-the-art and guide future progress. Conscience has now made the entire experimental dataset of the CACHE Challenge available to the public, including the chemical structures of all the molecules tested and associated computational methods. #Conscience #CACHEChallenge #ParkinsonsDisease #DrugDiscovery #ComputationalModels #OpenScience

Spatial biology is a rapidly growing field, but analysis is still problematic due to the complexity and time-consuming nature of analyzing multiplex images.   Turning multiplex images into biological results shouldn’t be a difficult task. That’s why we’ve developed a 7- step workflow to meet all your needs: Phenoplex.   💻 Watch the 18-min webinar with James R. Mansfield and learn about each step of the workflow, designed to efficiently evaluate spatial biology assays with 8-40 or more biomarkers.   Link to webinar: https://hubs.ly/Q02fNMX30   #Phenoplex #MultiplexImageAnalysis #SpatialBiology

Our defining advantage is demonstrated know-how analyzing large multimodal datasets (and queries into a data lake): our single-cell imaging solutions is a nice example👍. How can this accelerate your therapeutic program?

📢 Unlocking Spatial Biology: Overcoming Challenges with MACSima™ 🔍 The article discusses the challenges in spatial biology and how they can be overcome using the MACSima™ Platform. 🧬 Spatial biology combines different "omics" techniques to gain a deeper understanding of human health and disease. 🌟 The MACSima™ Platform enables the staining and imaging of a large number of targets, providing valuable insights into the tissue microenvironment. 🔬 Solid tumor cancers are particularly challenging, but the MACSima™ Platform can help characterize the tumor microenvironment. 🔍 Miltenyi Biotec offers a wide range of immunologically relevant antibodies for use with the MACSima™ Platform. 🧪 The platform also includes advanced informatics tools for data analysis and interpretation. 💡 By increasing marker availability, reducing panel optimization time, and enabling simple data analysis, the challenges in spatial biology can be addressed effectively. #BioprocessUpdates #SpatialBiology #MACSimaPlatform #OmicsTechniques #TissueMicroenvironment #SolidTumorCancers #ImmunologicallyRelevantAntibodies #DataAnalysis #InformaticsTools #MarkerAvailability #PanelOptimization #DataInterpretation #SpatialBiologyChallenges #MACSimaInsights #MiltenyiBiotec #DataAnalysisTools ▷ Read the full article here: 📎 https://lnkd.in/dkwib9CP

Explore groundbreaking insights from recent scientific research leveraging the ISS Alba V5 confocal laser microscopy system. Researchers have unveiled how the reprogramming factor KLF4 plays a pivotal role in chromatin organization through biomolecular condensation with DNA—a process crucial for inducing pluripotency in cells. This study, utilizing the precision of our ISS Alba V5 system, revealed that KLF4 forms liquid-like condensates with DNA, even in the absence of its intrinsically disordered region. Remarkably, this condensation is significantly enhanced by CpG methylation at specific DNA sites, such as the NANOG promoter, a key gene in maintaining pluripotency. Through advanced single-molecule Förster resonance energy transfer (smFRET) analysis, conducted with the Alba V5, the research team was able to monitor the interactions between KLF4’s DNA binding domain and the NANOG promoter. The smFRET data provided crucial insights into how KLF4-DNA interactions are modulated by CpG methylation, highlighting the intricate molecular dynamics that govern chromatin reorganization. This research underscores the vital role our ISS instrumentation plays in advancing the understanding of fundamental biological processes, offering scientists the tools they need to uncover the mechanisms behind cellular identity and reprogramming. Full Publication: https://lnkd.in/dMqmRPXx More about Alba V5: https://lnkd.in/ghZj3C_t #ChromatinOrganization #KLF4 #BiomolecularCondensation #Pluripotency #smFRET #ConfocalMicroscopy #CpGMethylation #Epigenetics #GenomicResearch #StemCellResearch #MolecularBiology #ISSInstruments #AlbaV5 #ScientificResearch #CellReprogramming