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Long-Sought CaE2P State Captured: Cryo-EM Illuminates SPCA1's Transport Cycle Secretory-pathway Ca2+-ATPases (SPCAs) are nature's tiny gatekeepers, controlling the flow of calcium ions within cells. These critical molecules ensure proper calcium concentrations, vital for diverse functions like cell signaling, muscle contraction, and protein processing. Understanding how SPCAs work, particularly human SPCA1 (hSPCA1), has remained a scientific puzzle. This research unveils a groundbreaking feat—capturing six snapshots of hSPCA1 in action, using an advanced technique called cryo-electron microscopy (cryo-EM). These snapshots, like frames in a movie, reveal the protein's intricate dance during calcium transport. From Fueling to Release Imagine hSPCA1 as a molecular pump, powered by the energy molecule ATP. The journey unfolds in these key stages: • Calcium Entry: Calcium ions bind to a specific pocket on hSPCA1, ready to be transported. • Fueling Up: ATP binds to the pump, injecting energy into the system. • Phosphorylation Boost: A phosphate group attaches to the pump, triggering conformational changes. • The Twist: An intriguing twist occurs—transmembrane helices shift, squeezing the calcium pocket and pushing the ions toward the other side of the membrane. • Release and Reset: Calcium ions are released into the target compartment, and the pump resets for another round. Unprecedented Moves What's remarkable is that hSPCA1's dance differs from other pumps. Its ATP binding and phosphorylation steps involve unique movements, highlighting the protein's specialized function. Additionally, the helix twist creates a powerful squeeze, a previously unseen mechanism for calcium release. The Missing Piece Found Moreover, this research captures the elusive CaE2P state, a crucial but rarely observed stage in the cycle. This missing piece adds clarity to the entire pumping process. Beyond the Lab Understanding hSPCA1's intricate work has exciting implications beyond basic science. Mutations in this protein are linked to Hailey-Hailey disease, a skin disorder. Deciphering its function paves the way for designing potential therapies and improving diagnosis. Furthermore, insights into SPCA1's unique pumping mechanism could inspire the development of novel biomimetic pumps for nanotechnology and drug delivery applications. In conclusion, this groundbreaking research unveils the hidden choreography of hSPCA1's calcium transport, offering a deeper understanding of cellular processes and opening doors for future innovations in healthcare and beyond. 📝 Article, Open Access https://lnkd.in/e6Cge2j9 📷 EM Map Analysis https://lnkd.in/eQTaWbij 📎 Free Use and License https://lnkd.in/gpbw3cEg 📌 About EM Data Bank https://lnkd.in/ePU9n4kv Wu M, Wu C, Song T, Pan K, Wang Y, Liu Z. Cell Res (2023) #disease #research #structuralbiology #merize

🇬🇧 Decoding a Cellular Powerhouse: Cryo-EM Reveals Secrets of Mammalian Respiratory Complex I at Near-Atomic Resolution Diving into the intricate machinery of life, researchers have made groundbreaking strides in understanding a vital cellular enzyme, mitochondrial NADH:ubiquinone oxidoreductase (Complex I), thanks to single-particle electron cryomicroscopy (cryo-EM). This complex molecule, responsible for pumping protons across membranes to generate energy, has long captivated scientists due to its sheer size and complexity. 🛟 #UnitedKingdom Unlocking the Details 🔹️ High-resolution map of mouse Complex I: In 2014, a 5-Å resolution map of bovine Complex I sparked the revolution. Now, scientists have achieved a remarkable 3.3-Å resolution model of mouse Complex I, encompassing 96% of its amino acids. This detailed map unveils nearly the entire structure, offering unprecedented insight. 🔹️ Diversity in action: Cryo-EM also revealed different particle classes, representing distinct functional states of the enzyme. Linking these states to known biochemical activities provides a deeper understanding of Complex I's dynamic nature. 🔹️ Collaborative effort for success: Improvements in various aspects, including protein purification, grid preparation, data collection, and image processing, were crucial for achieving this high resolution. Beyond the Map: Connecting to Ancient Relatives By comparing the mammalian Complex I structure to an ancient relative, membrane-bound hydrogenase, researchers gained valuable evolutionary insights. This comparison sheds light on the evolutionary origins and conserved mechanisms of this fundamental biological process. Impact and Future Directions This groundbreaking work provides a solid foundation for understanding Complex I's catalytic mechanism, paving the way for further research into its role in health and disease. Additionally, the ability to capture different functional states opens doors to studying dynamic aspects of enzyme function in unprecedented detail. Real-World Use Case Understanding Complex I's structure and function is crucial for developing targeted therapies for diseases associated with mitochondrial dysfunction, such as neurodegenerative disorders and certain cancers. By elucidating specific mechanisms and vulnerabilities, researchers can design drugs to modulate Complex I activity and potentially treat these currently incurable conditions. As technology continues to advance, we can expect even more exciting discoveries that will illuminate the intricate workings of life at the molecular level. 📝 Article, Open Access https://lnkd.in/e9MBhfrv 📷 EM Map Analysis https://lnkd.in/ebsjDTaF 📎 Free Use and License https://lnkd.in/gpbw3cEg 📌 About EM Data Bank https://lnkd.in/ePU9n4kv Agip AA, Blaza JN, Fedor JG, Hirst J. Annu Rev Biophys (2019) #disease #research #structuralbiology #merize

Today is the first step in a transformative journey to unlock the most in-demand skills in biochemistry. 🚀 Let me captivate your mind with a story of groundbreaking innovation: Imagine this: A young boy named Alex faced a devastating cancer diagnosis. Traditional treatments failed, and hope was slipping away. Then, a revolutionary breakthrough in Nanobiotechnology and AI in Medicine emerged. Researchers developed nanoparticles that targeted only cancer cells, guided by the precision of AI. Within months, Alex’s cancer was in remission. This is the power of merging nanotechnology with artificial intelligence. 🌟 🔬 Nanobiotechnology involves using nanoscale materials to solve biological challenges. From targeted drug delivery to advanced diagnostics, its potential is limitless. 💻 Artificial Intelligence (AI) amplifies these innovations by analyzing vast data, predicting outcomes, and designing efficient nanomaterials. The fusion of nanobiotechnology and AI is not just changing medicine—it’s creating a future where treatments are precise, effective, and personalized. If you’re driven by the pursuit of cutting-edge research and revolutionary applications, let’s connect and delve into these exciting advancements together! 🌐 Looking forward to learning and collaborating with you. #Biochemistry #Nanobiotechnology #AI #Medicine #Innovation #Networking #antibiotics #healthcare

🌟Shining Light on Quantum Dots🕵️♂️ 🪧The nanometer-sized semiconductor particles exhibit unique optical and electronic properties, making them invaluable in a variety of biomedical applications, from imaging to drug delivery. ▪️Quantum Dots: An Overview 🔄Quantum dots are tiny particles, typically ranging from 2 to 10 nanometers in diameter. 🔄They possess size-dependent optical properties due to quantum confinement, which allows them to emit light at specific wavelengths when excited. 🔄This tunable emission spectrum, along with their high brightness and photostability, makes QDs superior to traditional fluorescent dyes and organic molecules used in biomedical research. 💯Bioimaging with Quantum Dots 🔄One of the most promising applications of quantum dots is in bioimaging. Their bright and stable fluorescence allows for the creation of high-resolution images of biological tissues and cells. 🔄Quantum dots can be engineered to target specific cellular structures, enabling researchers to visualize complex biological processes in real-time. 🔄For instance, QDs conjugated with antibodies can bind to cancer markers, facilitating the early detection and diagnosis of tumors. 📍Recent advancements in QD technology have led to the development of near-infrared (NIR) emitting QDs, which penetrate deeper into tissues, providing clearer images with minimal background noise. 💯Quantum Dots in Targeted Drug Delivery 🔄Beyond imaging, quantum dots hold significant promise in the realm of targeted drug delivery. 🔄Due to their small size and surface modifiability, QDs can be designed to deliver therapeutic agents directly to diseased cells, minimizing side effects and enhancing treatment efficacy. By attaching specific ligands to their surface. RESEARCHER - ATHEENAPANDIAN Manoj_Atheenapandian_Researcher BioScience_Central VICE PRESIDENT - ATHEENAPANDIAN MOHAMMED SAHIL S-TRAINING OFFICER (TO)-ATHEENAPANDIAN Krithina - Trainer@Atheenapandian Dhanushya Biomedical Trainer ATHEENAPANDIAN PRIVATE LIMITED Aruna Biomedical Trainer ATHEENAPANDIAN PRIVATE LIMITED

Can’t agree more! Biological models have actually revolutionized the thinking perspective of scientific community and it has opened up a new horizon for discovery and learning. Very interesting insight!!! #animalmodels #3Dorganoidmodels #cellbasedmodels #invitrodiagnostics #invitromodels #computationalbiology #preclinicalbiology

🔬 𝐈𝐧𝐧𝐨𝐯𝐚𝐭𝐢𝐨𝐧 𝐢𝐧 𝐍𝐞𝐮𝐫𝐨𝐭𝐞𝐜𝐡𝐧𝐨𝐥𝐨𝐠𝐲: 𝐓𝐡𝐞 𝐈𝐦𝐩𝐥𝐚𝐧𝐭𝐚𝐛𝐥𝐞 𝐅𝐢𝐛𝐞𝐫 𝐎𝐩𝐭𝐢𝐜 𝐂𝐚𝐧𝐧𝐮𝐥𝐚𝐞 𝐌𝐚𝐫𝐤𝐞𝐭 🔬 𝐂𝐥𝐢𝐜𝐤 𝐇𝐞𝐫𝐞, 𝐓𝐨 𝐆𝐞𝐭 𝐅𝐫𝐞𝐞 𝐒𝐚𝐦𝐩𝐥𝐞 𝐑𝐞𝐩𝐨𝐫𝐭 https://lnkd.in/dJs999ZH The Implantable Fiber Optic Cannulae Market is at the cutting edge of scientific discovery, offering groundbreaking solutions in neurotechnology and biomedical research. These devices, used in optogenetics and neural imaging, are revolutionizing how researchers and clinicians study and manipulate brain activity with unparalleled precision. 🌟 Key Features: High Precision: Implantable fiber optic cannulae enable targeted light delivery to specific brain regions, allowing researchers to study neuronal circuits and behaviors with remarkable accuracy. Minimal Invasiveness: Designed for chronic implantation, these cannulae minimize tissue damage while maintaining long-term stability, making them ideal for longitudinal studies in both preclinical and clinical research. Advanced Materials: Utilizing biocompatible materials, these cannulae ensure safety and durability, even under extended use in complex neural environments. 📈 Market Dynamics: Rising Demand in Neuroscience: The growing focus on brain research, especially in areas such as optogenetics, deep brain stimulation, and neuroprosthetics, is driving the demand for implantable fiber optic cannulae. Cutting-Edge Applications: These devices are instrumental in understanding complex neurological disorders, including Parkinson’s disease, Alzheimer’s, and epilepsy, as well as in the development of novel therapeutic interventions. Technological Advancements: Ongoing innovations in miniaturization, light source integration, and data transmission are enabling more efficient and precise neural research methodologies. #Company Prizmatix Ltd. Thorlabs Doric Lenses Inc. Ainnotech Scitech Korea Inc. Plexon Inc Hangzhou Inper #Type • Ceramic • Stainless Steel • Others #Application • Hospital and Clinic • Laboratory • Other #Neurotechnology #BiomedicalResearch #Optogenetics #Neuroscience #MedicalDevices #Innovation #HealthcareTechnology #LinkedInPost

The dye in Doritos can make mice transparent This breakthrough, detailed in a study published in Science, could revolutionize medical imaging by allowing scientists to peer into tissues with unprecedented clarity. Tartrazine, also known as Yellow 5, a dye often used to color snacks and sodas, has been found to temporarily render mouse skin nearly transparent. When applied to the skin of hairless mice, the dye alters how light passes through the tissue, making organs, vessels, and muscles visible to the naked eye. This simple and reversible method could lead to advances in both microscopy and medical diagnostics, although much research remains before it can be applied to humans. The science behind this transparency involves fundamental physics principles. Tartrazine changes the refractive index of water in the tissue to match that of fat, reducing light scattering and allowing photons to travel through skin as if it were homogeneous. Researchers modeled this effect using various dyes before testing it on live mice and other samples, finding that Yellow 5 was the most effective. While the dye appears to cause minimal toxicity in mice, more safety studies are required before any potential human applications, such as early cancer detection or enhancing blood draws, can be explored. For now, the discovery offers a powerful new tool for research, particularly in improving the visibility of small animal models like mice in scientific studies.

Two research teams, led by Magdalena Götz and Boyan Bonev, have shown how glial cells are reprogrammed into neurons via epigenetic modifications. Using novel methods in epigenome profiling, they identified that a posttranslational modification of the reprogramming neurogenic transcription factor profoundly impacts epigenetic rewiring and the improvement in neuronal programming. They identified a novel protein as key player in this conversion process, namely the transcriptional regulator YingYang1 that physically interacts with the neurogenic factor to open up the chromatin. These novel insights reveal how the conversion at the molecular level works and pave the way to improve the reprogramming of glial cells into neurons. 🔗 You can find a summary and author insights on our website: https://lnkd.in/dyS8Qsqt LMU Munich – Ludwig-Maximilians-Universität München, Helmholtz Munich, Biomedical Center (BMC) LMU Munich

Biomedical nanotechnology is dedicated to exploring nanoscience and nanotechnology for health wellness, with the ultimate goal of personalized health management. The applications of siRNA-nanoparticle complexes cover numerous diseases such as cancer and viral infection. They have the ability to detect cancer due to the permeable nature of the tumor's blood vessels, which allows the nanoparticles to penetrate and accumulate in the tumor due to their small size. Metal oxide nanoparticles, for example, which produce a high-contrast signal on magnetic resonance imaging (MRI) or computed tomography (CT), can be coated with antibodies specific to membrane receptors found on cancer cells. Once inside the body, this system selectively binds to cancer cells and illuminates them for the scanner. Similarly, gold particles can be used to improve light scattering for endoscopic techniques such as colonoscopy. In this way, nanotechnological strategies can allow the visualization of molecular markers that identify stages and types of cancer, allowing doctors to see molecules and cells not detected by conventional imaging techniques. Recent research has revealed that the use of nanorobots to administer antitumor drugs maintains the concentration of the drug at the site during treatment and minimizes the effects on adjacent healthy cells or tissues. References; EGOROV, E. et al. Robotics, microfluidics GRODZINSKI, P. et al. MAZZEO, A.; SANTOS, E. J. C. MOORE, J. A.; CHOW, J.C.L.

💡 Don't miss the next Seminar at CIC nanoGUNE: 🔬 Advanced Imaging Techniques in in vivo models (Intravital Microscopy, Nuclear and Molecular Imaging).   The seminar will address advances in both intravital microscopy and preclinical nuclear (PET and SPECT) and molecular (MRI) imaging techniques, two fundamental approaches for biomedical research. Intravital microscopy allows real-time observation of cellular processes, such as cell movement and tumor progression, within living organisms. On the other hand, nuclear and molecular imaging techniques offer crucial anatomical and functional precision for studying diseases in areas such as oncology, cardiology, and neurosciences. Both technologies improve the understanding of complex biological processes and contribute significantly to translational research.   📅 Date: Monday 23 September 🕥 Time: 11:00 - 12:30 h 📍 Place: nanoGUNE   Speaker: Andrea Zapater, Ph.D. Product Specialist Bio.   Registration link: https://lnkd.in/dKh8XU9h Paralab Bio SL IVIM Technology Mediso Medical Imaging Systems