German Society for Extracellular Vesicles’ Post

🐴Strangles🐴 📋Causative Agent: Streptococcus equi subsp. equi. Involves the upper respiratory tract with abscessation of regional lymph nodes. More severe in horses 1–5 years of age. 🐎Transmission: Highly Contagious via upper respiratory purulent exudates or from discharging abscesses. Horses can shed the bacteria before developing clinical signs, or up to 6 weeks later. There is also a chronic carrier state when the bacteria are present in the guttural pouch. 🩺Clinical Presentation: • Multiplication in lymph nodes causing abscesses.  • High fever, depression, anorexia • Oculonasal discharge turning purulent • Swollen and painful head/neck lymph nodes • Submandibular nodes are affected and they can rupture discharging purulent, highly infectious material • Guttural pouch empyema is also a common finding 🔬Diagnosis: Culture or PCR of abscess material or washes from guttural pouch or nasopharynx. 🧫👩🔬This clinical case is of a submandibular abscess in a horse, purulent exudate was submitted to the laboratory, and after overnight incubation we could observe pure growth of small mucoid Beta-haemolytic colonies. MALDI-TOF identified the isolate as Streptococcus equi subsp. equi, and in the direct smear pictures of the pus, we can observe the characteristic long chains of Gram-positive cocci, along with many neutrophils. #veterinarymedicine #microbiology #equinehealth #medicinaveterinaria

Discovery of an Australian Chelonia mydas papillomavirus via green turtle primary cell culture and qPCR #marinevirology #herpetology #herpesvirologist #biomedicalscience #marinescience

We are thrilled to introduce Kaori Sakamoto, the wise and fearless Departmental Head of the CVM's Population Health and Pathobiology (PHP). The PHP Department oversees a wide breadth of CVM services including Ruminant Medicine, Clinical Pathology, Anatomic Pathology, Poultry, Swine, Immunology, Virology...and the list goes on! On top of her departmental duties, she will serve as a pathologist on our biopsy service alongside our residents. Keep reading to learn more about Dr. Sakamoto. 1. What is your favorite gross/histologic lesion and why? I have to say the tuberculosis granuloma, which is what I researched during my PhD. I just find the interaction between mycobacteria and macrophages fascinating, albeit frustrating, and I love a huge multinucleated giant cell! 2. What classes are you currently teaching and what are you currently researching? I believe I'll be teaching in immunology and the professional skills curriculum, but that hasn't been set in stone yet. 3. If you were a cell, what type of cell would you be and why? Hands down a macrophage. I LOVE to eat! 4. What is your favorite thing about working with the NCSU pathology team? It's a great, young group of people with lots of new ideas and energy!

An exciting study in Nature Communications (Nature Portfolio) entitled "Bacteriophage DNA induces an interrupted immune response during phage therapy in a chicken model" uncovered a fascinating aspect of phage therapy. While bacteriophages are traditionally considered safe due to their specificity for prokaryotic cells, they can elicit specific cellular responses in eukaryotic hosts. Bacteriophages administered during phage therapy are initially recognized by animal cells as viruses. However, a crucial pathway of the innate antiviral response, the cGAS-STING pathway, is interrupted at the IRF3 transcription factor phosphorylation stage. This inhibition arises from the inability of RNA polymerase III to recognize phage DNA and produce dsRNA molecules, which are essential for stimulating a protein complex necessary for IRF3 phosphorylation. These findings shed light on the mechanisms underlying the impairment of the antiviral response during phage therapy. Understanding the interaction between bacteriophages and the host immune system is pivotal for optimizing the safety and efficacy of phage therapy in both veterinary and human medicine. Read more 👉 https://lnkd.in/d6SkZJa5

[#BioguardProduct] Avian hepatic disease can present with vague symptoms, making it difficult to distinguish from other conditions. Although clinical enzymology is commonly used, it only indicates hepatocyte injury without revealing the chronicity or severity of the disease. Diagnostic imaging can help identify hepatomegaly and ascites, but a definitive diagnosis requires histological examination. Treatment for avian hepatic disease involves providing palliative care, addressing the specific illness, and creating an optimal environment for liver healing. Biochemistry results may show various anomalies, and our miniCHEM Biochemistry Analyzer efficiently analyzes key markers for precise results in just 12 minutes. This analyzer is tailored for various species with minimal sample volume.

📢 Excited to share our latest research published in the International Journal for Parasitology! 🐾 Our team investigated risk factors for the progression of Canine Leishmaniosis (CanL) in a cohort study spanning over 20 years. Highlights: 🔍 Risk factors shaping host-parasite interactions impact the clinical management of canine leishmaniosis. 👁️ Renal and ocular findings are major risk factors for LeishVet stages III and IV. 💡 Diagnosis of comorbidities is key to managing dogs with leishmaniosis, although independent of their clinical outcome. 🧬 A complex polygenic component underlies the development of severe clinical forms of leishmaniosis in certain purebred dogs. 🛡️ Protective immunity against *Leishmania* parasites is related to genetic factors involved in the endoplasmic reticulum (ER) stress and unfolded protein response (UPR) pathways. Congratulations to Carolina R. Sanz for this article, the third of her doctoral thesis. Read the full article here: https://lnkd.in/dSB9AEXC #CanineLeishmaniosis #Parasitology #Research #DogHealth #VeterinaryScience #Genetics #Immunity #DogCare #LeishVet #SciencePublication 📚

June's Case Of The Month just arrived! Thanks to NationWide Laboratories! This month's case follows a 10-year-old domestic shorthair male cat presented with a recurrent nasal mass, which was initially excised surgically last year. The clinical pathology team at NationWide Laboratories performed a cytological examination of the mass using digital pathology images, revealing marked pyogranulomatous inflammation and suspicious yeast elements that were likely to be Cryptococcus. The presence of round to oval (11-12 um diameter) yeast structures with internal basophilic stippling and a thin clear external halo were observed both extracellularly and within macrophages. There were also frequent pseudohyphae observed in some areas. This looked consistent with a diagnosis of Cryptococcus infection. The digital images allowed for high-resolution visualisation of the fungal elements; however, further confirmation would require culture or PCR testing to definitively identify the species and determine the specific genotype involved. Follow the link to read on... #digitalpathology #pathologynews #caseofthemonth

This #Arthritis study led by Tamara Dietrich and Tazio Maleitzke at the chariteberlin underscores the importance of evaluating animal health in preclinical research, commonly using body condition, weight, and grimace scales. However, nest-building behavior, a natural activity in mice, had not been previously studied in rheumatoid arthritis (RA) and osteoarthritis models. The researchers studied nesting behavior in wild-type and genetically modified mice with rheumatoid arthritis (RA) compared to healthy controls. Clinical parameters and nesting behavior were tracked over 10 or 48 days. For this study, the CAIA model induced with MD Bioproducts ArthritoMab Antibody Cocktail was applied, and wild-type (WT) or genetically modified rodents were examined. Results showed a slight positive link between nest score, body weight, and grip strength in affected mice, while no significant association was found between clinical arthritis score and ankle size. The study suggests that RA impacts, such as weight and strength loss, may reduce nesting behavior. Consequently, assessing nesting behavior could be a valuable, noninvasive method of monitoring mouse welfare, and providing preformed nests might aid mice with severe arthritis. Authors: Tamara Dietrich, Annette Aigner, Alexader Hildebrandt, Jérôme Weber, Mara Meyer Günderoth, Katharina Hohlbaum, Johannes Keller, serafeim tsitsilonis and Tazio Maleitzke. Find out more about their important study by click the link below. Learn more about the product used in this study: https://lnkd.in/g3kfNw8i #Inflammation #Immunology #AutoimmuneDiseases #DrugDiscovery

🔬 Exciting Discovery Alert! 🔬 My colleagues Dr. Ashrafi Tamai and Dr. Mansoor Lakooraj and I are thrilled to share our recent findings on a novel strain of the Feline Immunodeficiency Virus (FIV). Through our collaborative research efforts at the Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, we have identified a new strain, named "Feline Immunodeficiency Virus strain Iranian Pet. Hos114," with a unique envelope glycoprotein (env) gene sequence. 🐈 FIV, a lentivirus that affects domestic cats, has been a subject of extensive study due to its impact on feline health. Our discovery sheds light on the genetic diversity of this virus, providing valuable insights into its evolution and potential implications for feline populations. 🔍 Key Details: - **Strain Name:** Feline Immunodeficiency Virus strain Iranian Pet. Hos114 - **Envelope Glycoprotein Gene (env) Sequence:** Partial cds - **Accession Number:** MZ476768.1 - **Source:** Isolated from blood samples of felines in Iran - **Collection Date:** 2021 🌍 Our research underscores the importance of global collaboration in understanding and combating infectious diseases. We're grateful for the opportunity to contribute to the scientific community's knowledge base and look forward to further exploration in this field. #Research #Virology #FelineHealth #Collaboration #Science #Discovery

[#BioguardProduct] Avian hepatic disease can present with vague symptoms, making it difficult to distinguish from other conditions. Although clinical enzymology is commonly used, it only indicates hepatocyte injury without revealing the chronicity or severity of the disease. Diagnostic imaging can help identify hepatomegaly and ascites, but a definitive diagnosis requires histological examination. Treatment for avian hepatic disease involves providing palliative care, addressing the specific illness, and creating an optimal environment for liver healing. Biochemistry results may show various anomalies, and our miniCHEM Biochemistry Analyzer efficiently analyzes key markers for precise results in just 12 minutes. This analyzer is tailored for various species with minimal sample volume.