Ophthopedia’s Post

🔬 Recent studies have explored the molecular architecture of heart failure (HF). 🫀 Researchers are now turning to extracellular vesicles (EVs) as a potential "liquid biopsy" for HF. These tiny structures transport proteins, DNA, and RNA between organs, providing valuable insights into disease status and multiorgan function. Exciting findings reveal distinct RNA profiles in EVs between HFpEF and HFrEF, offering new possibilities for personalized management. 💡 #HeartFailureResearch #ExtracellularVesicles #LiquidBiops Saumya Das https://lnkd.in/g4SGX39V

🔬 Exploring the Iris Anterior Limiting Layer's Impact on Uveitis & Iris Tumors: 🎯 Unraveling the crucial role this layer plays in disease pathogenesis and potential breakthroughs. Join the discussion on how understanding this intricate. 🤓👁️ #Uveitis #OCTImaging #EyeHealth #MedicalResearch #ASOCT #ClinicalCases #InflammationInsights #retina #eye #ophthalmology Topcon Healthcare Young Uveitis Specialists The Ocular Immunology and Uveitis Foundation Uveitis National Clinical Study Group

This is such a beautiful work! Beautiful cryo-CLEM work on the Alzheimer's pathology. 🧠 🔬 "Madeleine A. G. Gilbert et. al. used cryo-fluorescence microscopy-targeted cryo-sectioning, cryo-focused ion beam-scanning electron microscopy lift-out and cryo-electron tomography, and determined in-tissue architectures of β-amyloid and tau pathology in a postmortem Alzheimer’s disease donor brain. β-amyloid plaques contained a mixture of fibrils, some of which were branched, and protofilaments, arranged in parallel arrays and lattice-like structures. Extracellular vesicles and cuboidal particles defined the non-amyloid constituents of β-amyloid plaques. By contrast, tau inclusions formed parallel clusters of unbranched filaments. "

Tubular mitochondria network in Multiple Sclerosis microglia To learn more about the role of nodules in MS patients, researchers investigated the pathology of MS in brain donors with and without nodules. These samples offered insights into the severity of the MS pathology of the patients’ level of inflammation. When the team took a closer look, they found that the nodules were absorbing and processing oxidized lipids, which is known to result in activation of these microglia. “In fact, when we looked at the axons surrounded by the nodules at high resolution, we saw that they were associated with partially demyelinated axons. These nodules likely arose to clean up the oxidized myelin. Nodules that are activated by both the lymphocytes and these oxidized lipids may become very inflammatory, causing more damage to the tissue surrounding them, leading to a sort of downward spiral.” The researchers quantified the mitochondria frequency and size in axons encapsulated by microglia nodules in MS and in controls. Of each microglia nodule, the translocase of the outer mitochondrial membrane (TOMM20) network was classified as fragmented, intermediate, or tubular. This tubular network is indicative of a hypermetabolic state of the microglia nodules in MS. #ScienceMission #sciencenewshighlights https://lnkd.in/g44bB96m

Ophthopedia Update:Decreased Macular Choriocapillaris Perfusion Correlates with Contrast Sensitivity Function in Dry Age-related Macular Degeneration.: To investigate the relationships between contrast sensitivity (CS), choriocapillaris perfusion and other structural optical coherence tomography (OCT) biomarkers in dry age-related macular degeneration (AMD). #Ophthalmology #Retina #Ophthotwitter

Ophthopedia Update:Evaluation of OCT biomarker changes in treatment-naive neovascular AMD using a deep semantic segmentation algorithm: Eye, Published online: 27 July 2024; doi:10.1038/s41433-024-03264-1Evaluation of OCT biomarker changes in treatment-naive neovascular AMD using a deep semantic segmentation algorithm #Ophthalmology #Eye #Ophthotwitter

CaseStudyOfTheDay _pathology360_ A 6-year-old boy presents to the clinic with a three-month history of persistent skin rashes, intermittent fever, and bone pain, especially in the skull and femur. His parents also report that he has been more irritable than usual and has experienced unexplained weight loss. On physical examination, multiple papular lesions are observed on his scalp , hand and trunk. Additionally, tenderness is noted over the skull and femur. Laboratory investigations reveal normocytic anemia and mild leukocytosis. An X-ray of the skull shows lytic lesions. A biopsy of one of the bone lesions is performed, and histopathological examination reveals cells with abundant eosinophilic cytoplasm and grooved, coffee-bean-shaped nuclei( shown ) . Further, Immunohistochemistry (IHC) shows positivity for CD1a, S100, and Langerin (CD207 ;corresponds with Birbeck granule formation in electron microscopy). Further genetic testing reveals the presence of the BRAF V600E mutation. State the MOST likely diagnosis; along with list of differential diagnosis. A #pathology360 Original. Associate Professor Dr Kishore G Banerjee, MD.

Selective inhibition of NHE1, a pH-regulatory protein, improved sensorimotor and cognitive deficits in a mouse model of repetitive TBI This study found that repetitive mild TBI in C57BL/6J mice stimulated neuroinflammation, caused axonal damage, upregulated the expression of the Na+/H+ exchanger isoform 1 (NHE1), an important pH-regulating plasma membrane protein, and induced neurological functional deficits. The administration of a potent NHE1 inhibitor, HOE642, following repetitive mild TBI, resulted in a reduction of pathological signatures, such as gliosis, oxidative stress, axonal damage, and white matter damage (as detected by MRI DTI) and improved locomotor and cognitive functional recovery. The authors concluded that targeting the NHE1 protein could be a potential therapeutic strategy for reducing mild TBI pathology and improving neurological functions. #TBI #acidosis #traumaticbraininjury https://lnkd.in/etBh9Tf4

In a recent highlight from our webinar, "Unlocking Genetic Causes of Sudden Cardiac Death with Scalable Assays of Variant Effect," Dr. Victoria Parikh provided an insightful answer to an important question about utilizing assays of variant effects To hear Dr. Parikh's response and explore more about how scalable assays are transforming our approach to cardiac genetics, click the link to view the entire session. Don't miss these expert insights into advancing precision medicine in cardiology! Watch the Full Webinar Here: https://lnkd.in/d5BFGbn3 #CardiacGenetics #PrecisionMedicine #Webinar #MAVEvidence #ConstantiamBiosciences #polygenicconditions