Join us next week for our next free webinar, New Advances in Extracellular Vesicle miRNA Research Using High-Throughput Affinity Capture, being broadcast on Wednesday, 28 February 2024, at 7.00 p.m. (GMT) if you would like to take part with the live questions and answers session. If it is too late, you will be able to hear this webinar on-demand the following week. Extracellular vesicles (EVs) are a diverse group of membrane-bound particles ranging from approximately 30 nm up to a few micrometres in diameter. These vesicles are released by cells into the extracellular space and biofluids, carrying molecular signals capable of influencing both proximal and distal cell functions. EV-associated signalling molecules include: nucleic acids; proteins; and lipids that are exposed on the vesicle surface, intercalated in the bi-lipid layer membrane or encapsulated within their lumen. The development of accurate and reliable EV-based diagnostics necessitates the identification of disease-associated biomarkers within specific EV subpopulations and the implementation of rapid, reproducible and scalable sample processing. Conventional isolation methods face challenges due to the co-isolation of particles with similar physicochemical properties. Promising alternatives involve methods targeting specific vesicle-surface epitopes, compatible with automated platforms. Join our featured speaker, Dr Greg Rice from INOVIQ Ltd (ASX:IIQ) as he identifies challenges associated with translating our understanding of extracellular vesicle (EV) biology into clinically useful applications and to highlight potential solutions. In this webinar, you will learn about: • The advantages of using vesicle-surface epitope affinity capture for enriching EVs • How to combine EV enrichment and RNA isolation into a seamless rapid process • How to fully automate EV enrichment and downstream analysis to achieve high-through-put sample analysis Register at https://bit.ly/3wlYWzw to reserve your place #Genomics #miRNA #HTS
Promega UK’s Post
More Relevant Posts
-
Register for the second webinar on AVITI24: Accelerating Discovery Through Integrated Sequencing and Cytoprofiling. AVITI24 pushes benchtop sequencing into the next frontier by re-imagining core sequencing components, such as avidity base chemistry (ABC) and high-resolution imaging, to enable comprehensive multiomic analysis by Teton™ CytoProfiling, all on one platform. During this webinar, you will: Enhanced AVITI24 Core Sequencing - with two billion reads across dual flow cells and up to Q50 data quality with UltraQ™ AVITI24 with Teton CytoProfiling - analyze DNA, RNA, proteins, phosphorylated proteins, and cell morphology simultaneously, from just one sample in <24 hours Application across various cell types Register now for insights into the future of sequencing! https://lnkd.in/g-VWDq_S Want to revisit the first webinar on Empowering Multiomic Cytoprofiling and Cell Morphology Analysis on AVITI24™ using Teton™ Chemistry here - https://lnkd.in/gcXudpeA #aviti #ngs #dna #genomics #biomarkers #apoptosis #cellcycle #singlecellanalysis #dnasequencing #proteomics #cellbiology #cytoprofiling #cellsignalling #medicalresearch #lifesciences
-
-
Could ML be beneficial in AAV capsid modification? Definitely! 💻 The approaches of viral capsid engineering are greatly diverse and embrace even directed evolution. In terms of AAV, it implies mutating Cap genes using error-prone PCR, DNA recombination, or conventional ligation that is followed by selection with high-affinity antibodies against capsid or tissue structures of interest. Directed evolution of AAV is not limited only to the capsid itself but also includes the insertion of random peptides in the latter. Such a strategy significantly expands the boundaries of vector variability. However, there is always the probability of a stop codon occurring. To avoid such a problem, the NNK method is to be used. It implies putting any of four nucleotides in the first two positions of a codon and only G or T (K) in the third position. Danqing Zhu et al. used ML to create an AAV5 library with a variable seven–amino acid insertions within 575–577 positions in the viral protein. As a result, they achieved a fivefold increase in package capacity in comparison to the NNK approach. Great achievement! 👏 The ML-based library design for the directed evolution of AAV capsid is provided by the authors in the paper. ML in creating libraries DOI: 10.1126/sciadv.adj3786 Review on directed evolution in AAV capsid engineering DOI: 10.1038/nbt1182 Random peptide libraries DOI: 10.1038/nbt856 The presented figure was taken from this paper #aav #genetherapy #capsid_modification #biotechnology
-
-
Looking for ways to improve your scRNA-seq results? The answers to your challenges can be found at the Mid-Atlantic Single Cell Symposium at the University of Maryland on March 13th. Industry leading experts will share their insights to help you improve your scRNA-Seq and snRNA-Seq workflows for better downstream results. Seating is limited, so don't wait to register! #sequencing #singlecell #scRNAseq #genomics https://hubs.la/Q02lNkxh0
-
Excited to share new research on enhancing CNV detection in Whole Exome Sequencing with Twist Bioscience! 🧬🔬 Our team at SeqOne evaluated the Twist Exome 2.0 Plus combined with Twist CNV Backbone Spike-in Panels for improved copy number variant (CNV) detection. The results are impressive: - 100% sensitivity in detecting duplications and deletions across all tested resolutions - 96% mean overlap between expected and detected CNV regions - Effective detection of CNVs ranging from 100kb to 150Mb Why does this matter? Accurate CNV identification can significantly increase diagnostic yield in genetic testing. This approach allows labs to transition from microarray-based CNV detection to a more comprehensive NGS workflow. The SeqOne platform offers key benefits for labs looking to implement this advanced CNV detection in their workflows: - CE-IVD certified for confident clinical use - Intuitive interface for efficient data interpretation - Seamless integration & automation with various sequencing platforms - Powered by explainable AI for enhanced variant prioritization Check out the full poster for more details on our methods and results and feel free to reach out if you want to learn more. We're proud to contribute to advancing genomic diagnostics and improving patient care. Chung-Ting (Tina) Han Céline Gottin Denis Bertrand Tiffany Delhomme Jérôme Audoux Michael Blum Alessandro Davassi Nicolas Philippe #Genomics #Bioinformatics #GeneticTesting #NGS #Diagnostics
-
On the Aggregation of AAVs Curt Jarand and a team from Tulane University along with Karen Baker, Matthew Petroff et al from Spark Therapeutics, Inc. just published a very nice piece of biophysical characterization work on the use of real time #DLS to study #AAV aggregation! 👏 Their newest paper explores how DNA influences the aggregation of AAV capsids. The team revealed that AAVs containing #DNA show significantly slower aggregation compared to empty AAVs. The timecourse on aggregation is particularly impressive! As the industry knows, there’s a host of orthogonal ways to continue studying these aggregates and their degradation pathways. #HPLC #SEC #MALS and #CDMS to name a few. 📄Tulane/Spark Paper: https://lnkd.in/e_rrQZPh #GeneTherapy #Biotechnology #CGT #GeneTx 🧪
DNA Released by Adeno-Associated Virus Strongly Alters Capsid Aggregation Kinetics in a Physiological Solution
pubs.acs.org
-
Time to replace Array-based Comparative Genomic Hybridization (Array-CGH) or Chromosomal Microarray Analysis (CMA)? Check out enrichment with Twist Bioscience Exome 2.0 Plus + CNV Backbone Spike-in Panel and data analysis with SeqOne for detecting small, medium, and large CNV events all in one assay!
Excited to share new research on enhancing CNV detection in Whole Exome Sequencing with Twist Bioscience! 🧬🔬 Our team at SeqOne evaluated the Twist Exome 2.0 Plus combined with Twist CNV Backbone Spike-in Panels for improved copy number variant (CNV) detection. The results are impressive: - 100% sensitivity in detecting duplications and deletions across all tested resolutions - 96% mean overlap between expected and detected CNV regions - Effective detection of CNVs ranging from 100kb to 150Mb Why does this matter? Accurate CNV identification can significantly increase diagnostic yield in genetic testing. This approach allows labs to transition from microarray-based CNV detection to a more comprehensive NGS workflow. The SeqOne platform offers key benefits for labs looking to implement this advanced CNV detection in their workflows: - CE-IVD certified for confident clinical use - Intuitive interface for efficient data interpretation - Seamless integration & automation with various sequencing platforms - Powered by explainable AI for enhanced variant prioritization Check out the full poster for more details on our methods and results and feel free to reach out if you want to learn more. We're proud to contribute to advancing genomic diagnostics and improving patient care. Chung-Ting (Tina) Han Céline Gottin Denis Bertrand Tiffany Delhomme Jérôme Audoux Michael Blum Alessandro Davassi Nicolas Philippe #Genomics #Bioinformatics #GeneticTesting #NGS #Diagnostics
-
𝗣𝗿𝗼𝗳𝗲𝘀𝘀𝗼𝗿 𝗼𝗳 𝗠𝗼𝗹𝗲𝗰𝘂𝗹𝗮𝗿 𝗣𝗵𝘆𝘀𝗶𝗼𝗹𝗼𝗴𝘆 @TUM School of Life Sciences in Weihenstephan 🧬🧬🧬 Liquid Biopsy; Dx Biomarker Development; qPCR, NGS & EV Specialist; Gene Quantification Events
Our latest review in ‘Molecular Aspects of Medicine', Vol 97, June 2024, 101269 Special issue 'Modern Methods of Molecular Diagnostic' 𝗔 𝗽𝗶𝗽𝗲𝗹𝗶𝗻𝗲 𝗳𝗼𝗿 𝘁𝗵𝗲 𝗱𝗲𝘃𝗲𝗹𝗼𝗽𝗺𝗲𝗻𝘁 𝗮𝗻𝗱 𝗮𝗻𝗮𝗹𝘆𝘀𝗶𝘀 𝗼𝗳 𝗲𝘅𝘁𝗿𝗮𝗰𝗲𝗹𝗹𝘂𝗹𝗮𝗿 𝘃𝗲𝘀𝗶𝗰𝗹𝗲-𝗯𝗮𝘀𝗲𝗱 𝘁𝗿𝗮𝗻𝘀𝗰𝗿𝗶𝗽𝘁𝗼𝗺𝗶𝗰 𝗯𝗶𝗼𝗺𝗮𝗿𝗸𝗲𝗿𝘀 𝗶𝗻 𝗺𝗼𝗹𝗲𝗰𝘂𝗹𝗮𝗿 𝗱𝗶𝗮𝗴𝗻𝗼𝘀𝘁𝗶𝗰𝘀 https://lnkd.in/dWP-GfNU #extracellularvesicles #moleculardiagnostics #qPCR #RNAsequencing #bioinformatics #liquidbiopsy #biomarkers #exosomes
Mapping out the EV-transcriptomic workflow: in this review article, Christian G., Michael W. Pfaffl at Technical University of Munich and collaborators established a methodology for creating and analyzing transcriptomic biomarkers associated with extracellular vesicles https://lnkd.in/eYkQJPJE Their process involves the recruitment of patients and proceeds with the collection and processing of blood samples, followed by purification and characterisation of EVs, along with the isolation and quality assessment of EV-associated RNA. The authors outlined the essential stages of this methodology and underscore the importance of quality control checkpoints, which are aligned with various available guidelines that should be adhered to throughout the process. An article also authored by Martina Schuster, Dr. Florian Brandes, Agnes S. Meidert, Benedikt Kirchner, PD Dr. Marlene Reithmair and Gustav Schelling #extracellularvesicles #exosomes #RNA #liquidbiopsy #bioinformatics #Vesiculab
-
-
Follow our MPXMonday series to learn about Molecular Pixelation!
We have been talking about Molecular Pixelation (MPX) workflows up until now on #MPXMonday. We have a series of videos where our CEO, Simon Fredriksson, explains our technology and its features in detail. Today we learn about the chemistry behind Molecular Pixelation. Watch to understand the technology and the method in detail. If you can't wait and want to learn more: https://lnkd.in/dmT7PXJ8 #MPXMonday #molecularpixelation #MPX #immunology #PBMCs #Tcells #Bcells #spatialbiology #spatialanalysis #bioinformatics #bioinformatician #biochemistry #dnasequencing #NGSsequencing #Illumina #newtechnology #sequencing #pixels #cellsurface #cellsurfaceproteins #surfaceproteins #immunologypanel #proteins #immuneresponse #immunesystem
-
You could run this exact experiment on Uncle and directly measure size by DLS, confirm aggregation by SLS, DNA leak by fluorescence with SYBR Gold. And with higher throughput than the other instruments listed. Details here: https://lnkd.in/eb54K2SB
On the Aggregation of AAVs Curt Jarand and a team from Tulane University along with Karen Baker, Matthew Petroff et al from Spark Therapeutics, Inc. just published a very nice piece of biophysical characterization work on the use of real time #DLS to study #AAV aggregation! 👏 Their newest paper explores how DNA influences the aggregation of AAV capsids. The team revealed that AAVs containing #DNA show significantly slower aggregation compared to empty AAVs. The timecourse on aggregation is particularly impressive! As the industry knows, there’s a host of orthogonal ways to continue studying these aggregates and their degradation pathways. #HPLC #SEC #MALS and #CDMS to name a few. 📄Tulane/Spark Paper: https://lnkd.in/e_rrQZPh #GeneTherapy #Biotechnology #CGT #GeneTx 🧪
DNA Released by Adeno-Associated Virus Strongly Alters Capsid Aggregation Kinetics in a Physiological Solution
pubs.acs.org
-
From monogenic to multigenic analysis: a paradigm-shifting revolution 💡 Discover how recent advancements in next-generation sequencing technologies and data analysis resources enabled a shift towards multigenic studies in an attempt to tailor therapies for patients 🦠 Read about this study on autoinflammation and immunological dysregulation, performed by employing a multigenic enriched analysis through Clinical Exome Sequencing, and the identification of genes responsible for the modulation of inflammation via post-translational modifications. 🧬🔬 We at 4bases harness the power of NGS in our new ClinEx pro sequencing kit, allowing for the analysis of over 10,000 genes described as related to known pathologies, and embodying our commitment for precision medicine! 👉 Click here to discover the full article: https://lnkd.in/d496Bf8q #NGS #PrecisionMedicine #HealthcareInnovation
-
