Adhesion and Inflammation Lab - LAI (INSERM U1067 | CNRS UMR 7333)

📢 Check out the recent contribution on, Morphodynamics of T-lymphocytes: Scanning to spreading Abstract: Binding of the T cell receptor complex to its ligand, the subsequent molecular rearrangement, and the concomitant cell-scale shape changes represent the very first steps of adaptive immune recognition. The first minutes of the interaction of T cells and antigen presenting cells have been extensively scrutinized; yet, gaps remain in our understanding of how the biophysical properties of the environment may impact the sequence of events. In particular, many pioneering experiments were done on immobilized ligands and gave major insights into the process of T cell activation, whereas later experiments have indicated that ligand mobility was of paramount importance, especially to enable the formation of T cell receptor clusters. Systematic experiments to compare and reconcile the two schools are still lacking. Furthermore, recent investigations using compliant substrates have elucidated other intriguing aspects of T cell mechanics. Here we review experiments on interaction of T cells with planar artificial antigen presenting cells to explore the impact of mechanics on adhesion and actin morphodynamics during the spreading process. We enumerate a sequence tracing first contact to final spread state that is consistent with current understanding. Finally, we interpret the presented experimental results in light of a mechanical model that captures all the different morphodynamic states. For more details 👇 Reference: Sengupta, K., Dillard, P., & Limozin, L. (2024). Morphodynamics of T-lymphocytes: scanning to spreading. Biophysical Journal. 👉 https://lnkd.in/d4UcWEgN Kheya Sengupta Pierre Dillard Laurent Limozin CINaM, Centre Interdisciplinaire de Nanoscience de Marseille Adhesion and Inflammation Lab - LAI (INSERM U1067 | CNRS UMR 7333) #TCellBiology #Immunology #Morphodynamics #TCellActivation #AdaptiveImmunity #CellMechanics #AntigenPresentation #Biophysics #CellAdhesion #ActinDynamics #LigandMobility #ImmuneRecognition #BiomedicalResearch #CellularInteractions

📢 Check out the recent contribution on, Antigen density and applied force control enrichment of nanobody-expressing yeast cells in microfluidics Abstract: In vitro display technologies such as yeast display have been instrumental in developing the selection of new antibodies, antibody fragments or nanobodies that bind to a specific target, with affinity towards the target being the main factor that influences selection outcome. However, the roles of mechanical forces are being increasingly recognized as a crucial factor in the regulation and activation of effector cell function. It would thus be of interest to isolate binders behaving optimally under the influence of mechanical forces. We developed a microfluidic assay allowing the selection of yeast displaying nanobodies through antigen-specific immobilization on a surface under controlled hydrodynamic flow. This approach enabled enrichment of model yeast mixtures using tunable antigen density and applied force. This new force-based selection method opens the possibility of selecting binders by relying on both their affinity and force resistance, with implications for the design of more efficient immunotherapeutics. For more details 👇 Reference: Sanicas, M., Torro, R., Limozin, L., & Chames, P. (2024). Antigen density and applied force control enrichment of nanobody-expressing yeast cells in microfluidics. Lab on a Chip, 24(11), 2944-2957. 👉 https://lnkd.in/dciZviKU Merlin Sanicas Rémy Torro, Ph.D. Laurent Limozin Adhesion and Inflammation Lab - LAI (INSERM U1067 | CNRS UMR 7333) Patrick Chames CRCM - Centre de Recherche en Cancérologie de Marseille #Microfluidics #Nanobodies #YeastDisplay #AntibodySelection #Biotechnology #Immunotherapy #MechanicalForces #CellBiology #InVitroTechnologies #ResearchInnovation #Bioengineering #ForceResistance #AntigenDensity #BiomedicalResearch #Biophysics #lifescience

🌟 Comic Alert! 🌟 We're excited to share "The AI Academy: Training, Testing, Triumph" by Chandrasekar SUBRAMANI NARAYANA (Adhesion and Inflammation Lab - LAI (INSERM U1067 | CNRS UMR 7333) / Laboratoire d'Informatique et Systèmes - LIS Qarma team), with stunning illustrations by Alexandre Brodovitch (CRN2M), as part of the scientific outreach initiative by CENTURI - Turing Centre for Living Systems This comic takes you on a journey through the fascinating process of developing AI models for research. It explains the critical stages of training, validation, testing, and generalization, shedding light on how these steps help in analyzing complex data and predicting outcomes. Don't miss out on this insightful and engaging story! 📚✨  check out more comics by other researchers:https://lnkd.in/dmPx_itQ #AI #MachineLearning #DataScience #Research #ScienceCommunication #Outreach #PhDlife #Innovation

🌟 Comic Alert! 🌟 We're excited to share "The Adventures of TEC and T" by Jessica Chevallier (Adhesion and Inflammation Lab - LAI (INSERM U1067 | CNRS UMR 7333), CIML, TAGC), with stunning illustrations by Alexandre Brodovitch (CRN2M), as part of the scientific outreach initiative by CENTURI - Turing Centre for Living Systems. Join TEC and T on an epic journey through the thymus, the vital organ where T cells develop and learn to become the immune system’s superheroes. Whether it's attacking cancer cells or fighting infections, none of this would be possible without TEC – the T cell's trusted ally. This comic beautifully illustrates the critical role of TEC in T cell development and delves into what happens to these two best friends as we age. Don't miss out on this insightful and engaging story! 📚✨ #Immunology #TCells #TEC #Thymus #CancerResearch #InfectionFighters #ScienceCommunication #outreach #PhDlife

🌟 Comic Alert! 🌟 We're excited to share "The Adventures of TEC and T" by Jessica Chevallier (Adhesion and Inflammation Lab - LAI (INSERM U1067 | CNRS UMR 7333), CIML, TAGC), with stunning illustrations by Alexandre Brodovitch (CRN2M), as part of the scientific outreach initiative by CENTURI - Turing Centre for Living Systems. Join TEC and T on an epic journey through the thymus, the vital organ where T cells develop and learn to become the immune system’s superheroes. Whether it's attacking cancer cells or fighting infections, none of this would be possible without TEC – the T cell's trusted ally. This comic beautifully illustrates the critical role of TEC in T cell development and delves into what happens to these two best friends as we age. Don't miss out on this insightful and engaging story! 📚✨ #Immunology #TCells #TEC #Thymus #CancerResearch #InfectionFighters #ScienceCommunication #outreach #PhDlife

Nous avons un financement pour un stage de Master2 à Marseille sur un sujet à l'interface immunologie-oncologie-physique: Vidéonanoscopie des interactions moléculaires au cours de la leucémogenèse dans le thymus.

🎉 LAI celebrated its newest Dr! on the 28th of June 2024, Lama Awada successfully defended her work titled "Imprint of mechanical forces on antibody affinity maturation in B cell immune responses" in front of a jury composed of experts in the field: Katelyn Spillane (Imperial College London) Paolo Pierobon (Institut Cochin)                         Rodolphe Jaffiol (L2n, Université de Technologie de Troyes)                                Kheya Sengupta (CINaM, Centre Interdisciplinaire de Nanoscience de Marseille)                                     under the supervision of Philippe Robert  from Adhesion and Inflammation Lab - LAI (INSERM U1067 | CNRS UMR 7333) and Pierre Milpied from CIML Associated with CENTURI - Turing Centre for Living Systems #congratulations Lama Awada and we can't wait to see what you achieve next! #phd #phdlife #phdstudent #sciencenews #research #biology #physics #biophysics #cells #PhDGraduation #AcademicJourney Aix-Marseille University

🌟 Seminar Hosted by LAI 🌟 We are thrilled to share a highlight from our recent seminar held today, June 28th. Katelyn Spillane from the Department of Life Sciences at Imperial College London presented an insightful talk titled "Physical Regulation of B Cell Activation in the Immune Synapse," discussing the mechanics of B cell activation in the immune synapse. Her talk was highly engaging and included an interactive question session, allowing attendees to delve deeper into the topic and discuss the latest findings. We extend our heartfelt thanks to Katelyn for her valuable contribution. #Biophysics #cellbiology #cell #Bcell #Immunology #BCellActivation #LAISeminar #LifeSciences #Research #ScienceTalk #Talk #ImperialCollegeLondon

🌟 LAI Celebrated International Yoga Day🌟 On this International Yoga Day, we're delighted to join millions around the globe in celebrating the profound practice of yoga. LAI came together to perform various yoga postures, the invigorating Surya Namaskar (Salutation au Soleil), and calming breathing exercises. This year marks the 10th International Yoga Day (Journée Internationale du Yoga), with the theme “Yoga for Self and Society.” Participation in the yoga day was focused on relaxing, rejuvenating, and contributing to our overall health and well-being. This experience not only helped us unwind but also reinforced the importance of integrating wellness practices into our daily lives. We encourage everyone to take a moment to connect with themselves through yoga and experience the tranquility it brings. Let's embrace the practice of yoga and its benefits together. #InternationalYogaDay2024 #YogaForSelfAndSociety #Yoga #HealthAndWellness #SuryaNamaskar #BreathingExercises #LAIYogaDay

🌟 Presentation at Laboratoire d’Informatique et Systèmes PhD Students Day! 🌟 We are excited to share that Chandrasekar SUBRAMANI NARAYANA recently participated in the LIS (Laboratoire d’Informatique et Systèmes) PhD Students Day on June 7, 2024. Chandrasekar presented his research titled “Counting Phage: Decoding Antibody & Antigen Kinetics Behavior,” which explores counting tasks in fluorescence microscopy images and count-guided localization using deep learning and computer vision. This event provided an excellent platform for sharing interdisciplinary scientific interests and knowledge, while exploring the diverse array of topics investigated by fellow PhD students and research teams. It also facilitated the development of interdisciplinary projects, fostering collaboration and innovation between laboratories. We are happy to share Chandrasekar's contributions and look forward to the innovative advancements his work will bring to the field! 👉https://lnkd.in/d7WEHUsA #PhDStudents #Research #DeepLearning #ComputerVision #InterdisciplinaryResearch #FluorescenceMicroscopy #Innovation #Collaboration #ScientificResearch #LISPhDDay