Evizia Inc.

#Evizia has been posting on PRECYSE’s primary application of NGS QC. One of our early access collaborators asked if we could see the polymerase bound to the DNA before sequencing. Indeed, this is something PRECYSE can do very well. Per a 2017 Nature Communications paper https://lnkd.in/eP2FYiFF Evizia’s co-founder Dr. Jason Reed et al. published a DNA mapping technique using CRISPR-Cas9, whereby the protein is clearly visible due to its significantly larger z-axis height compared to DNA. This technique was also used as an early confirmation of linear DNA sizing with the instrument. We imaged several #Oxford_Nanopore_Technologies (#ONT) and #PacBio fully constructed libraries from collaborators with and without either the helicase (ONT) or polymerase (PacBio) addition before sequencing. All images generated by PRECYSE can be viewed in 2D or 3D, whereby the z-axis height of molecules from the consumable background surface is color coded. One of many really cool capabilities of PRECYSE! …and maybe this will unlock our ability to better optimize NGS enzyme concentration and binding for sequencing.

Evizia awarded Virginia Catalyst grant to support commercialization of PRECYSE

#Evizia continues to work with early access collaborators running Long Read Sequencing (LRS) library samples for #PacBio and #OxfordNanopore (#ONT) on the PRECYSE instrument. We’ve been gathering feedback on how best to display sizing information. With this innovative technology we are directly imaging and measuring 40-800+DNA molecules from a given sample, thus the data is not indirect (DNA ladder) RFU mass-based sizing everyone is used to seeing from electrophoresis systems. In the LRS world small molecule populations can be a problem and are severely underrepresented in electropherograms due to small mass (limited RFUs). Unanimous feedback, and we agree, is a sizing histogram that allows direct comparison to read length N50 and mean insert size data generated by LRS instruments. Here is a sample provided by the #BROAD_Institute where the Femto generated a mean of 43.7kbp. PRECYSE showed a 12.2 kbp mean correlating nicely with the ONT N50 of 14.6 kbp. We consistently see high percentages of 1-5kbp DNA in LRS libraries, even after many commonly used size ‘clean-up’ techniques.

Evizia attended and presented a poster at The Jackson Laboratory Long Read Sequencing Workshop #LRSW2004. We'd like to thank the organizers Dr Mark Adams, Dr Christine Beck, Dr Olga Anczukow and Dr Chew Yee Ngan for the opportunity to present long read focused QC data from several early access customers utilizing the PRECYSE platform. There were a lot of great discussions and excitement among the #NGS attendees regarding the technology to be commercialized later this year. https://lnkd.in/dWUHKcKP

Evizia has been working with several leading US Academic #genomics research cores to further test the PRECYSE instrument with gDNA and #NGS library samples. The image below is a #PacBio library provided by a collaborator in Boston. A really clean beautiful library! Sizing comparison to Agilent #Femto: PRECYSE:  431 molecules analyzed in a 4min run (150+ molecules typically needed for statistically accurate sizing) Mean size = 9.0kb Femto: max RFU peak = 13.9kb. Avg insert size from PacBio sequencing data = 10.2kb. We consistently see that sizing by electrophoresis utilizing fluorescence overestimates the actual size of DNA, increasingly so for larger molecules.

Have you ever actually seen a #NGS #Illumina library ready to be loaded onto a flow cell? Of course not, because it’s too small to see…until now with the PRECYSE instrument! 

Really appreciate all the great discussions at #AGBT on the #Evizia PRECYSE instrument for NGS QC, DNA mapping, and so many other applications and utilization ideas I received feedback on!

Excited to share our approach for sequencing QC at #AGBT with Jason Reed, bud mishra, Brian Patterson,VIPC | Virginia Innovation Partnership Corporation , Magdalena Morgan, PhD , Virginia Commonwealth University, Alex Euler

Excited to share our approach for sequencing QC at #AGBT with Jason Reed, bud mishra, Brian Patterson,VIPC | Virginia Innovation Partnership Corporation , Magdalena Morgan, PhD , Virginia Commonwealth University, Alex Euler

Evizia Inc. and co-founder Jason Reed will be presenting a novel technology for #NGS sample and library #QC at AGBT - Advances in Genome Biology and Technology 2024 in Orlando, FL. The instrument and analysis software utilizes High Speed Atomic Force Microscopy (HSAFM). Please join us at Poster 600 on Tues. Feb 6, from1:30-3:30. For more information or to set up a time to meet at AGBT please contact Brian Patterson; patterson@eviziadx.com