Nanion Technologies’ Post

Ever wondered how cells communicate?? 🤔 Exosomes, a component of the heterogenous milieu of extracellular vesicles, are at the forefront of intercellular communication and disease biomarker research. Researchers Jordan Ngo, Justin Williams, Isabelle Lehman, and Randy Schekman at University of California, Berkeley have designed a protocol for in vitro exosome secretion reconstitution involving Streptolysin O (SLO)-permeabilized cells. The protocol effectively quantifies exosome secretion using a sensitive luminescence-based assay. 🌟 Learn more about the technique here https://lnkd.in/dCcu8wH9 and complement your own exosome-based research work! #ExosomeResearch #CellCommunication #Biomarkers #luminescence Contribute to scientific clarity and reproducibility by sharing your protocols with Bio-protocol, classic or novel. Explore publishing opportunities at https://lnkd.in/dJrqztsf #bio #protocol #bioprotocol #reproducibility #openaccess

🚀 Exciting news in Liver-Brain Axis research! Thrilled to share our latest Application Note on the development of an in vitro model of the Liver-Brain Axis using NANOSTACKS™. 👉 Dive into our latest Application Note, and into our previous achievements: https://lnkd.in/ey6yvjRY Key Highlights from the Application Note: - Comprehensive protocol for establishing the in vitro model - Functional characterisation of the liver cells HepaRG® included in the model - Significant advancements in recapitulating physiological responses Why NANOSTACKS™? - Cutting-edge technology for precise and reproducible results - Mimic the in vivo microenvironment for authentic cellular responses - Accelerate your research with confidence 📣 Join the conversation! Share your thoughts and questions in the comments. Let's propel liver-brain axis research together! #LiverBrainAxis #NANOSTACKS #ComplexInVitroModel #organonachip #DrugTesting #Revivocell

In this study, read about on a novel and comprehensive in vitro assay for studying T cell dynamics in real-time. MIMETAS' assay offers unparalleled insights into the complex processes of T cell trafficking, arrest, extravasation, and migration. Key highlights from the study include:  ✨A 3D perfused Endothelium-on-a-Chip, 3D ECM migration and a co-culture with tumor cells. ✨ Real-time visualization of primary human T cell behavior under flow conditions. ✨ Induction of transendothelial migration (TEM) through TNFα-mediated inflammation and CXCL12 gradients.  ✨ Unique insights into how T cell activation states influence migratory behavior. ✨ High throughput, quality imaging, and user-friendly design for seamless experimentation. Read the full publication here: https://lnkd.in/gKUANCZe #ImmunoOncology #CancerResearch #Organonachip 

Voxcan, part of the ERBC GROUP, specializes in non-invasive high-resolution imaging for non-clinical drug development. We offer advanced preclinical biodistribution studies using in vivo fluorescence imaging. ➡️ Our techniques enable real-time observation of labelled compounds' biodistribution with optimized NIR fluorochromes in both naïve and disease models. Combining bioluminescence with fluorescence imaging, we provide insights into compound colocalization with pathological processes over time. #3Rs #biodistribution #drugdevelopment #NAM #bioluminescence #fluorescenceImaging

🔍 Wondering why preclinical imaging should be on your radar? 💡 This video breaks it down for you! From unraveling intricate biological processes to accelerating drug development, preclinical imaging is your gateway to invaluable insights! With MOLECUBES, you can benefit from compact and intuitive imaging solutions with the highest performance supported by fast and simple workflows. State-of-the-art modular benchtop solutions from MOLECUBES make molecular imaging more accessible to a broader community of researchers in the field of preclinical drug discovery and development and fundamental and translational research. 👉 Ready to elevate your research game? Visit our website to learn more! #MolecularImaging #PreclinicalImaging #PreclinicalResearch

Prof. Chris Flask and his team at the Case Center for Imaging Research (CCIR) at Case Western Reserve University in Cleveland, Ohio are bringing preclinical and clinical research together. How? Download the customer insight "Imaging Serves as a Scientific Hub to Unite Preclinical and Clinical Research" to find out: https://lnkd.in/gFyHdRVu #Imaging #MRI #Preclinical #PreclinicalImaging

📢 ERBC offers advanced preclinical biodistribution studies using non-invasive in vivo fluorescence imaging. Our experienced team has experience in following a wide range of test items: ✅Nanoparticles ✅Nanomaterials ✅Proteins ✅Antibodies ✅Liposomes This technique allows to follow labelled-compounds biodistribution in live animals over time, using optimized NIR fluorochromes in both naïve and disease models. By combining bioluminescence with fluorescence imaging, we can assess compound colocalization with pathological processes longitudinally. Enhance your drug development with our state-of-the-art imaging technologies! #3Rs #biodistribution #drugdevelopment #NAM #bioluminescence #fluorescenceimaging

A new technology called "molecular pixelation" has been developed by researchers from Karolinska Institutet and Pixelgen Technologies, enabling the mapping of proteins within individual cells more comprehensively than before. Unlike traditional methods like flow cytometry that analyze a limited number of proteins, this technique allows for simultaneous analysis of hundreds of proteins, providing detailed insights into their distribution and interactions within the cells. This advancement is poised to significantly enhance our understanding of cellular processes and disease mechanisms, and it will aid in drug development and other medical research areas. For more details, you can read the work from Filip et al Nature Method 2024

Really excited to share our latest preprint via a #KeedyLab / Advanced Science Research Center, GC/CUNY collaboration with the #FarooqiLab / University of Cambridge. We investigated whether structural characterization of human #PTP1B variant proteins might reveal precise mechanisms to target for weight loss therapy. 12 rare PTP1B variants were selected for functional characterization from exomes from 997 people with persistent thinness and 200,000 people from UK Biobank. Seven of 12 variants impaired PTP1B function by increasing leptin-stimulated #STAT3 phosphorylation in cells. Using room-temperature X-ray #crystallography, hydrogen-deuterium exchange mass spectrometry (#HDX), and computational modeling, we determined that human variants modulate the 3-dimensional structure of PTP1B through distinct #allosteric conduits that energetically link distal, highly ligandable structural regions to the active site. Our study highlights the value of coupling human genetics and structural biophysics for targeted drug discovery, and hope to use these studies as a foothold to inform the design of allosteric PTP1B #inhibitors for the treatment of obesity. Check it out right here!

Check out my interview with SelectScience® in case you missed the opportunity to stop by Nanolive's booth or Tech Talk at the ELRIG UK Drug Discovery Conference. I explain how Nanolive SA's unique imaging & analysis technology works, and how it can be used for novel drug discovery insights, in under 5 mins. #livecellimaging #drugdiscovery #metabolism #cellbiology