Promega UK’s Post

Our blog post summarizes how polarized cell monolayers are the gold standard for studying P-gp and BCRP interactions as indicated in regulatory guidances and as such should be the first choice of test system for identifying P-gp or BCRP substrates and inhibitors. However, if results indicate the compound has inherently low passive permeability then P-gp or BCRP expressing membrane vesicles should be considered as an alternative follow up in vitro test system to mitigate against the clinical implications of false negatives. Read the blog on the Evotec Science Pool here - https://hubs.ly/Q02bYRt20 #researchneverstops

Our blog post summarizes how polarized cell monolayers are the gold standard for studying P-gp and BCRP interactions as indicated in regulatory guidances and as such should be the first choice of test system for identifying P-gp or BCRP substrates and inhibitors. However, if results indicate the compound has inherently low passive permeability then P-gp or BCRP expressing membrane vesicles should be considered as an alternative follow up in vitro test system to mitigate against the clinical implications of false negatives. Read the blog on the Evotec Science Pool here - https://hubs.ly/Q02bYRML0 #researchneverstops

Curious about the future use of blood microsampling in decentralized clinical trials to support the development of antisense oligonucleotides therapies? Find out how our scientists achieved precision, accuracy, and linearity within acceptance criteria using our sensitive and selective bioanalytical method for determining ASOs. https://lnkd.in/dyK3vUzz #Bioanalysis #MassSpectrometry #Microsampling

📣 Final reminder! Our Vice President of Sales Enablement and Customer Experience, Alyssa Master, PhD, will be taking the main stage at BioProcess International to share Cell Culture Solutions to Unlock Your Cell Performance. The presentation includes a case study on taking a custom media formulation to GMP while enhancing the vital characteristics needed by a cell therapy developer. Learn how media can be your advantages! #bpi #cellculture #custommedia #bpi23

Discover how our cutting-edge NanoBRET® Target Engagement (TE) Assay is revolutionising drug discovery in a new article. Gain valuable insights from one Promega scientist and learn how #NanoBRET TE is successfully bridging the biochemical and functional cellular assay divide. Read here: https://bit.ly/3Urb0Yl

MEDY enables the #cryopreservation of cortical organoids without compromising their structure or function. Is this a success? This new technique (methylcellulose, ethylene glycol, DMSO, and Y27632) preserves crucial features, maintaining synaptic function and preventing harmful cellular processes. Its adoption is poised to transform research, medical diagnostics, and drug development by offering a cost-effective way to store these vital resources for future studies. Our CEO Dr. Emil Kendziorra "It is noteworthy to observe the growing number of research groups engaging in neuropreservation and the expanding interest in this field; this milestone serves as a testament to the increasing recognition of its importance. We can confidently view this as further validation of the expertise in the field." 🔗 Find the paper here: https://ow.ly/OI5g50RTNwm 📷 Survival of progenitor cells and neurons in day 90 cortical organoids after MEDY cryopreservation through Sox2 and MAP2 immunofluorescence staining. Scale bars, 100 μm.

Speed up your demyelinating disease drug discovery! Access to a scalable source of oligodendrocytes is incredibly challenging, creating a bottleneck in discovering new therapeutic targets and drug candidates for demyelinating diseases like #MultipleSclerosis. Our precision reprogrammed human iPSC-derived ioOligodendrocyte-like cells™ are ideal for screening compounds that modulate myelination with an 8 day protocol, due to the rapid expression of O4 and MBP. This protocol offers an alternative to difficult cell generation protocols that can take up to 3 months, allowing scientists to quickly and consistently generate oligodendrocyte-like cells to accelerate drug discovery programs. Try a vial now! https://hubs.ly/Q029D9-b0 #research #DrugDiscovery #cell #glia #iPSC #optiox #ioCells #leukodystrophy #neurodegeneration #screening

Our Whole Blood Processing Kits are validated for 136 antibodies and counting! 🔬🧪 This extensive validation ensures high compatibility and reliability for your research needs. This guarantees that critical cellular and molecular markers are preserved with minimal hands-on time and virtually no specialized training. Contact us to get the complete list of validated antibodies and compare your panel. Enhance your research with Cytodelics! #Research #Biotech #FlowCytometry #MassCytometry #SamplePreservation #Cytodelics

More than 90% of therapeutic candidates fail to make it past phase III clinical trials, mainly due to the lack of adequate, advanced cell models to provide in-depth predictive insights that can translate into positive clinical outcomes. This free half-day virtual event on January 23, 2024, will feature three presentations on challenges of #organoid cultures and #assays, tips for automating workflows, and ensuring reproducible and scalable 3D cell cultures. Register now: https://ow.ly/GPbn50Qo0H1 #3dOrganoids #3dSpheroids #ipsc #LiveCellAnalysis

#METABOLITE #GPCR sensing - how #ketones/beta-hydroxybutyrate; mono-methylfumarate (the #MS drug), and #niacin work. Amazing how the puzzle of 30 (thirty) new #HCAR2 structures when pieced together by Asli (Aslihan Shenol Iliyaz), Ricardo Tenente, Michael Lückmann, and Thomas Frimurer shows the whole beautiful picture of the electrostatically guided, complex agonist binding path into a totally occluded orthosteric site in the middle of the receptor; how an #agoPAM surprisingly works by controlling the dynamics of the entry/exit gate; and how a two-way allosteric mechanism connects G protein with agonist binding. G protein binding ALONE stabilizes ALL major conformational changes - including extracellular loop 2b - except only the induced-fit anchoring of agonist in the orthosteric pocket. Just released from Nature Comm - https://lnkd.in/d-wHFq8V But - if you want not just to look at the glossy ‘frozen’ pictures outside the movie theater, but would like to watch the real movie, i.e. the heavy #moleculardynamics simulations and #metadynamics analysis of metabolite binding (in that case #succinate), you should check out Asli’s paper (also many nice pictures) from the April issue of Mol. Cell https://lnkd.in/dNSAd-5g It is a true privilege - still - to be allowed to work with talented and fun students like Asli - submitting her thesis this weekend - and Ricardo at Novo Nordisk Foundation Center for Basic Metabolic Research - thank you!