Congratulations to The Structural Genomics Consortium (SGC) scientists on this work demonstrating how the NanoBRET® Target Engagement System can be miniaturized and automated. By streamlining the workflow for kinase selectivity screening in live cells, this approach can make cellular kinase profiling more accessible. This could be a game changer for researchers tackling complex kinase biology questions. https://lnkd.in/g_navfNw
A new protocol demonstrates how a single commercial kit enables cost-efficient, live-cell kinase selectivity screening. Unlike traditional in vitro assays, this method accounts for cellular factors such as membrane penetration, ATP competition, and protein interactions. By profiling over 100 inhibitors, this approach reduces reagent costs and eliminates the need for purified proteins, making large-scale screening more accessible. Read more: https://lnkd.in/gDms6j9w #KinaseScreening #DrugDiscovery #NanoBRET #OpenScience