BioIVT’s Post

Replacing Western blotting, a case study at Cardiff University / Prifysgol Caerdydd In this video case study, Dr Tracey Martin, a Senior Lecturer at Cardiff University, discusses her recent N-WASP drug discovery project, where slow and expensive Western blots were replaced with Lumit™ Immunoassay Cellular Systems. The team were able to generate results in under two hours whilst also improving both the reliability and consistency of data generated. Read the full case study at: https://bit.ly/3PxiyXj #LabWork #WesternBlotting #Lumit

The work of my PhD has been finally published on the Journal of Medicinal Chemistry! The project was based on the investigation of the mGlu7 receptor NAM XAP044 to discover its binding mode in the Venus Flytrap Domain of mGlu7R and to synthetize more potent analogs. The combination of SAR, pharmacology and molecular modelling studies allowed to disclose a novel inhibitory site in the mGlu7R VFT domain. Furthermore, more potent analogs of XAP044 have been discovered. All these results give us a deeper knowledge of the mGlu7 receptor mechanism and their role in future drug discovery! Thanks to all the people who worked on this project: Alexandre Cabayé, Isabelle Brabet, Ralf Glatthar, Amelie Tora, Cyril Goudet, Hugues-Olivier Bertrand, Anne Goupil-Lamy, Peter J. Flor, Jean-Philippe Pin, Isabelle McCort-Tranchepain and Francine C. Acher. https://lnkd.in/eVSw5gra

#METABOLITE #GPCR sensing - how #ketones/beta-hydroxybutyrate; mono-methylfumarate (the #MS drug), and #niacin work. Amazing how the puzzle of 30 (thirty) new #HCAR2 structures when pieced together by Asli (Aslihan Shenol Iliyaz), Ricardo Tenente, Michael Lückmann, and Thomas Frimurer shows the whole beautiful picture of the electrostatically guided, complex agonist binding path into a totally occluded orthosteric site in the middle of the receptor; how an #agoPAM surprisingly works by controlling the dynamics of the entry/exit gate; and how a two-way allosteric mechanism connects G protein with agonist binding. G protein binding ALONE stabilizes ALL major conformational changes - including extracellular loop 2b - except only the induced-fit anchoring of agonist in the orthosteric pocket. Just released from Nature Comm - https://lnkd.in/d-wHFq8V But - if you want not just to look at the glossy ‘frozen’ pictures outside the movie theater, but would like to watch the real movie, i.e. the heavy #moleculardynamics simulations and #metadynamics analysis of metabolite binding (in that case #succinate), you should check out Asli’s paper (also many nice pictures) from the April issue of Mol. Cell https://lnkd.in/dNSAd-5g It is a true privilege - still - to be allowed to work with talented and fun students like Asli - submitting her thesis this weekend - and Ricardo at Novo Nordisk Foundation Center for Basic Metabolic Research - thank you!

🔬 𝗗𝘆𝗻𝗮𝗺𝗶𝗰 𝗧 𝗰𝗲𝗹𝗹 𝗯𝗶𝗻𝗱𝗶𝗻𝗴 𝗰𝗮𝗽𝘁𝘂𝗿𝗲𝗱 𝗟𝗜𝗩𝗘 🔬 This close-up view of Nanolive's LIVE T Cell Assay shows us exactly where and when T cells interact with a target cell (blue). Detected interaction sites are yellow, and unbound T cell surfaces are red. This 10 minute LIVE T Cell Assay captured by the lab of Dr. Eliana Stanganello at TRON-Mainz, was used to quantify T cell and target cell interactions over time. Learn more about the Live T Cell Assay here https://bit.ly/3Eo7tT2 #nanolive #biotechnology #celldeath #sciencecommunication #medtech #innovation #medicine #immunooncology #technology

This review explores the promising concept of "molecular glues" in drug development. These small molecules can selectively bind two different proteins, enhancing their interaction. By acting as intermediaries, molecular glues have the potential to modulate key protein-protein interactions involved in disease pathways. This ability to manipulate crucial cellular processes opens up new therapeutic avenues. As highlighted in "Molecular Glues Bring Partners Together," this emerging approach presents exciting opportunities for targeted drug discovery and precision medicine. The authors also listed several molecular glue-related deals. Article link: https://lnkd.in/gDuEbev4 #MolecularGlues #DrugDiscovery #PrecisionMedicine

Happy to share our novel work have been published in Q1 JOURNAL, 5.5 IMPACT FACTOR. Curcumin nanogels: promising therapeutic agent

🔬 Exciting News! Our latest test results are in, and we are thrilled to share that our product has been proven completely safe for use in the human body! 🎉 We conducted the MTT Assay, a widely recognized test for cytotoxicity measurement. The MTT assay is used to measure cellular metabolic activity, serving as an indicator of cell viability, proliferation, and cytotoxicity. The results of the MTT assay showed an impressive 93% viability of fibroblast cells after 24 hours for our #Biotechlie medical gauze. This demonstration of excellent biocompatibility surpassed the commercially purchased medical gauze and control samples. According to ISO 10993-5 guidelines, cell viability percentages above 80% are considered non-cytotoxic. Our product's viability percentage surpassed this threshold, confirming no cell toxicity of PDMS and CNF. However, further analysis is required to provide conclusive evidence. At #biotechline, we are committed to rigorous testing and delivering products you can trust! 💼 #biotechline #ProductSafety #MTTAssay #Biotechnology #Healthcare #Biocompatibility

ESTIV news: James McKim will be presenting new methodologies for drug discovery and safety using his MPS platform.   Check out his poster, "A Novel Meso-Scale MPS Integrated Organ Platform to Evaluate Usnic Acid Kinetics, Organ Distribution, and Cytotoxicity: A Case Study."   Don't miss session 3b on Tuesday, June 4th at 11am, where Jim and Kaushal Joshi will present their research on "A Pilot Study to Evaluate the Kinetics, Metabolism, and Toxicity of Coumarin in an In Vitro Three-Organ Integrated MPS Model."   See you there!   #ESTIV2024 #LifeSciences #NAMs #MPSmodel

#MDPIPharmaceutics Special Issue "Nanosystems for Drug Delivery" is open for submissions. Guest Editor: Prof. Dr. Xiaojia Chen, Dr. Wangxiao He, Prof. Dr. Qi Xiang Read more: https://shorturl.at/s1NDr The aim of this Special Issue is to gather the latest studies on peptide self-assembly, peptide–drug conjugates (PDC), nano-carriers for peptide delivery, in vitro and in vivo evaluations of their stability and biological activity. Original research papers, communications, or review articles are welcome for this Special Issue.

Recently, I read two papers on lymphatic filariasis (LF) discussing Cofactor-independent phosphoglycerate mutase (iPGM) as a potential drug target for LF, published in Oxford Academy-2024 (PMID:37481257) and Bentham Publications-2023 (PMID:31244425). I'm glad to have discovered this back in 2015. Here are some insights from my original article if you're interested: https://lnkd.in/eTdnyXs3. We delved into the structural stability of Wb-iPGM through molecular dynamics simulations, shedding light on its interaction with cofactors 3-phosphoglycerate (3PGA) and Mn2+ ions. Notably, we unveiled domain motions and the catalytic significance of Mn2+ ions, offering insights crucial for drug development. #LymphaticFilariasis #DrugDiscovery #ResearchInsights #Structuralbiology #simulations #bioinformatics #computationalbiology